Evaluation of the effects of Cannabis on cell growth of stem cells derived from endometrial tissue of uterine rats

Background: Cannabis is a psychedelic substance that is being abused by millions of people the world. The aim of this study was to investigate the effect of cannabis on the growth of mesenchymal stem cells(msc) derived from endometrium in adult rats. Materials and Methods: In this experimental study, msc were extracted from the endometrium of rats and after culturing and confirming the mesenchymal nature of the cells by flowcytometry and by expressingCD34 and CD90 and not expressingCD105 markers,in the third passage of cell culture, the effects of cannabis in concentration of100 and1000ng/ml were calculated on the growth of these cells within 1to8days by the PDT=T×ln2/ln⁡〖Xe/Xb〗 formula and the results were analyzed using ANOVA and Tuki tests.Results: Cells isolated from the endometrium adhered to the floor of the cell culture flask 24hours after transfer. The mesenchymal nature of these cells was confirmed by the expression of CD90, CD105 and non-expression of CD34markers.The results of cell counts also showed the growth of cells treated with cannabis until the third day of treatment similar to the control group. From the fourth day in the treatment group with a dose of100ng/ml cannabis increased significantly top