DIFFERENCES IN HEMATOXYLIN EOSIN (HE) STAINING RESULTS ON KIDNEY, SKIN AND COLON HISTOLOGY OF MICE (Mus musculus) BASED ON CUTTING THICKNESS OF 3 μm, 6 μm and 9 μm)
0
Citation
6
Reference
10
Related Paper
Abstract:
Influencing factors absorption coloring Hematoxylin Eosin (HE) is one of them size thickness cutting network, cuts are not appropriate and on time coloring that is not appropriate causes the absorption process color No perfect so that moment observation microscopic view of the cell nucleus and cytoplasm seen more pale and faint . Research objectives This is For know difference results coloring Hematoxylin Eosin (HE) on histology kidneys , skin , and colon mice (Mus musculus) based on thickness piece 3 µm, 6 µm, and 9 µm microtomes . Research methods This use method Experimental with nine groups treatment that is thickness cutting 3 μm , 6 μm and 9 μm microtome, then preparation done HE staining and observed quality microscopic includes the cell nucleus, cytoplasm and uniformity color. Data collection uses primary data, reading field done with 400x magnification (40x objective). Data processing uses statistical tests Mann Whitney . The results of the Mann Whitney test show the preparation kidney (p<0.05) that there is difference in a way significant , preparation skin cuts of 3 µm and 6 µm ( p > 0.05) that No There is difference significant cuts of 6 µm and 9 µm, 3 µm and 9 µm ( p< 0.05) that there is difference in a way significant, colon preparation ( p<0.05) that there is difference in a way significant. Conclusion of study This is in the kidneys cutting 3 µm microtome results preparation with quality the best coloring compared to with cutting 6 µm and 9 µm microtome , on skin and colon organs cutting 6 µm microtome results preparation with quality the best coloring compared to with cutting 3 µm and 9 µm microtome.Keywords:
Microtome
Eosin
Histology
Sectioning is a step that must be passed before staining Hematoxylin Eosin (HE). The aim of this study was to determine differences in the results of Hematoxylin Eosin (HE) staining in mice skin histology (Mus musculus) based on the thickness of the microtome sections of 3 μm, 6 μm, and 9 μm. Experimental research, true experimental research design post test only control group design. The research sample was mice skin preparations (Mus musculus). Primary data collection, cutting of skin preparations using a microtome and staining Hematoxylin Eosin (HE). Field readings were carried out at 400x magnification (40x objective). Data was processed using Kruskal Wallis and Man Whitney. The average value of 6 μm nuclear cutting was 1.44, cytoplasm was 1.15 and color uniformity was 3. The results showed an abnormal distribution, the Kruskal Wallis test (p=0.008) there was a difference in the quality of staining in the 3 μm, 6 μm and 9 μm. The Man Whitney test for the 3 μm and 6 μm microtome cutting groups (p=0.412) showed no difference in the group, the 6 μm and 9 μm microtome cutting groups (p=0.004) there were differences in the group, the 3 μm and 9 μm cutting groups ( p=0.004) there was a difference in the cutting group. The conclusion of this study was that 6 μm microtome cutting produced preparations with better staining quality compared to 3 μm and 9 μm microtome cutting.
Microtome
Eosin
Histology
Cite
Citations (0)
Instead of two-stage staining with hematoxylin-eosin and hallocyanin-picrofuchsin a technique for simultaneous staining in mixtures of hallocyanin and eosin or picrofuchsin is suggested. The stains-cocktails are well preserved for 4-5 weeks and may be used repeatedly.
Eosin
Eosin Y
Cite
Citations (0)
A standardized thionin-eosinic acid stain was developed as a quick and highly reproducible staining method for bronchial cytology. Bronchial smears and paraffin-embedded sputum samples were stained with thionin-eosin and with the conventional hematoxylin-eosin Y. Spectral absorption characteristics and staining intensity of thionin-eosin-stained cells were investigated by means of cytophotometry. The staining pattern of thionin-eosin is very close to that of the hematoxylin-eosin stain; the contrast between nucleus and cytoplasm is significantly higher for thionin-eosin. Thionin-eosin can be used for "dye-fixation" of cytologic smears and tissue imprints. Blueing and differentiation (as for hematoxylin-eosin) is not required for thionin-eosin; thus, fixation and staining can be performed within two minutes. The spectral absorption characteristics of thionin-eosin allow reliable automated cytophotometric discrimination of cell nuclei and cytoplasm. The standardized thionin-eosin stain is recommended as a substitute for the hematoxylin and eosin stain in bronchial cytology.
Eosin
Stain
Eosin Y
Cite
Citations (8)
Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the staining preparations results as good as those using hematoxylin.
Methylene blue
Eosin
Cite
Citations (2)
Microtome
Brain tissue
Cite
Citations (32)
Background:
Optimising hematoxylin and eosin (H&E) protocol does not only ensure reproducible and excellent staining quality, it also helps to optimise the use of lab materials and resources. This study investigates the effect of hematoxylin pH on H&E staining quality.
Method:
In an experimental study, 3 μm tissue sections of formalin-fixed paraffin embedded intestinal tissues of three healthy female Sprague-Dawley rats were subjected to the standard H&E protocol. Modified Gill’s hematoxylins of four pH (2.5,
2.75, 3.0 and 3.5) and eosin (pH 5.0) as counterstain were used.
Results:
At hematoxypin pH 2.5, the tissues appeared acidophilic with indistinctive epithelial lining. At pH 2.75, the hematoxylin dye was most prominent with good balance of staining, and crisp epithelial lining was observed. At pH 3.0 or higher, although the lining was crisp, blue-staining mucin were observed.
Conclusion:
pH 2.74 is the most optimal pH to achieve balance of coloration and definitive epithelial lining in H&E staining.
Eosin
Eosin Y
Cite
Citations (0)
Introduction: Most synthetic dyes are carcinogenic, and chronic exposure to these dyes has an impact on the health of laboratory technicians and pathologists. Eosin, a widely used synthetic dye in routine histopathological staining, poses potential risks. Zingiber officinale contains phenols and several colouring compounds that have the ability to stain tissues. Aim: To explore and compare the staining efficacy of ginger extract as a natural dye with synthetic eosin dye. Materials and Methods: This cross-sectional study was conducted at the Oral Pathology Department of Ahmedabad Dental College and Hospital from April 2023 to June 2023. Fresh rhizomes of Zingiber officinale were collected and airdried. A staining solution of Zingiber officinale was obtained by dissolving 25g of powder in 90% alcohol. This solution was used to stain 30 sections of oral biopsy specimens. The stained slides were evaluated by two independent observers using various parameters such as nuclear and cytoplasmic detail, overall histologic appearance, intensity, and contrast. Statistical significance was determined using the Chi-square test. Results: Z.officinale (Ginger) stain the cytoplasm of the cell and connective tissue elements with pale eosin colour, yellowish golden to the RBCs and deep brownish to the bony tissue. Statistical analysis comparable staining intensity, contrast, nuclear staining, cytoplasmic staining and overall histologic appearance between the two groups with p-values of 0.531, 0.917, 1.000, 0.924 and 0.7003, respectively. Conclusion: Z.officinale (Ginger) can be utilised as a natural alternative to eosin in routinely used Hematoxylin & Eosin staining.
Haematoxylin
Eosin
Zingiber officinale
Stain
Cite
Citations (2)
A hematoxylin and eosin procedure is described for staining formalin-fixed tissues embedded in glycolmethacrylate with emphasis on 1.5 micron sections. The staining method utilizes Gill's hematoxylin followed by a buffered eosin Y-phloxine B counterstain. Acidophilic and basophilic components of most stained tissue sections had an intense, sharp and clean appearance when observed by light microscopy.
Eosin
ALIZARIN RED
Stain
Basophilic
Eosin Y
Cite
Citations (5)
[Objective] To improve the staining technic of semi-sections with resin embedded and to be applied in pathology diagnosis and fine structure study.[Methods]The semi-sections with resin embedded were stained using multicolor and single staining methods.[Results] In multicolor staning sections there were contrast,similar to haematoxylin and eosin staining,then It can be used in many kinds of tissues for staining.Single staining sections were absence of levels and no contract,but it was better for nerve and muscle tissues.[Conclusions ]It was clear,fresh and like haematoxylin and eosin staining in multicolor semi-sections with resin embedded,it had integration characters with high resolving and many kinds optics staining,the best paraffin sections were cannot compare with it in cellular fine structra observed.
Haematoxylin
Eosin
Cite
Citations (0)
Objective To compare the staining quality between rapid hematoxylin and eosin (H&E) staining and routine H&E staining of frozen breast tissue sections. Methods In this cross-sectional observational study, 120 frozen breast tissue sections were randomly assigned to rapid or routine H&E staining ( n = 60 per group). Rapid H&E staining used a 7:1 mixture of modified Gill’s hematoxylin and alcohol-soluble 1% eosin Y. The staining quality of each section was evaluated and scored. A score of >7 was considered excellent, a score of 6 to 7 good, and a score of ≤5 poor. Results The staining time for rapid staining was approximately 3 minutes, whereas that of routine staining was approximately 12 minutes. There were no significant differences in the staining quality scores or proportions of sections in each grade between the two staining methods. The proportions of sections that were classified as excellent or good were 96.7% and 98.3% for rapid and routine staining, respectively. Conclusions In frozen breast tissue sections, rapid H&E staining may provide staining quality that is comparable to that of routine staining, while markedly reducing the staining time.
Eosin
Cite
Citations (1)