A Hematoxylin-Eosin Phloxine Stain for Tissues Embedded in Glycol Methacrylate
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A hematoxylin and eosin procedure is described for staining formalin-fixed tissues embedded in glycolmethacrylate with emphasis on 1.5 micron sections. The staining method utilizes Gill's hematoxylin followed by a buffered eosin Y-phloxine B counterstain. Acidophilic and basophilic components of most stained tissue sections had an intense, sharp and clean appearance when observed by light microscopy.Keywords:
Eosin
ALIZARIN RED
Stain
Basophilic
Eosin Y
ABSTRACT A method for protein determination using eosin Y is described. Eosin Y reacts with proteins under acidic conditions and forms an intense pink proteineosin Y complex with an absorbance maximum of 535-545nm. The color produced from this reaction is stable and its absorbance increases proportionally over a wide range of protein concentrations (standard assay, 50-1000μg/ml of BSA; microassay, 1.25-50μg/ml of BSA). The eosin Y method can be performed at room temperature without further incubation after vortex mixing. The assay has advantages such as (i) simple procedures, (ii) stable dye-protein color with no precipitation of dye-protein complex for at least 72h, (iii) good reproducibility and (iv) rapid reaction. The characterization of the eosin Y method extends its best application to a routine protein determination in solution.
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Absorbance
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Abstract The γ-radiolysis of eosin has been studied in deaerated neutral and alkaline methanolic solutions. Eosin is reduced principally with CH2OH radicals in dilute solutions. In neutral solutions, G(–eosin) is about 2 at the eosin concentration of 10−5m and increases as the concentration of eosin is increased. The final products are leuco-eosin and an unidentified substance which has an absorption peak at 455 nm. In an alkaline solution (10−5m), G(–eosin) is about 4 and the product is semireduced eosin, which is stable in the dark and which undergoes photo-debromination. The ESR spectrum of semireduced eosin has also been recorded. Comparative studies have also been made on uranin solutions.
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Eosin Y
Eosin
Xanthene
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UV/Fenton Catalyst system was used to treat Eosin B in simulated dye wastewater developed here.Studies showed that when the initial concentration was 50mg/L,at 25℃,the best condition was that pH=4.5 =0.54mol/L /≈1/29(Mol ratio) t=100min.Under this optimized condition,the decolorization rate was 99.7% above.The initial concentration of Eosin B and different effect of cation and anion on its decolorization were studied.Finally,comparing before and after UV spectrum,the results showed that the degradation of Eosin B was completed.
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Eosin
Degradation
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Globally, dyes are widely used in the pharmaceutical, food, textile, cosmetics, plastics, leather, paint, ink and paper industries. Eosin is an acidic orange-pink dye and has very strong staining properties. Haematoxylin and eosin Y (H&E) combination is the most common staining and primary diagnostic technique in histo-pathological laboratories. This review mainly discussed the utility of eosin dye in quality control laboratories as an ion pairing agent for drug analysis. Eosin Y is one the most common ion pairing agent and its mono and di anionic forms of eosin Y are capable of interacting with many drug molecules to form colored/fluorescent binary or ternary complexes that can be analyzed with or without extraction by spectrofluorimetry and/or spectrophotometry. Quenching fluorescence and advantages of spectrofluorimetry over spectrophotometry were also discussed. Fluorescence detection greatly enhances the sensitivity and providing a sensitive and relatively inexpensive instrumental method of analysis using eosin Y for various important drugs in pure, commercial dosage forms and biological fluids
Eosin
Eosin Y
Haematoxylin
Rose bengal
Spectrophotometry
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Suitable methods for the determination of eosin and ethyl eosin in samples of the latter dye are described. They depend upon the possibility of precipitating the color acid and measuring the same gravimetrically. What proportion of the dye is actually ethyl eosin and what proportion is eosin Y can be determined by making use of the great difference in solubility of the two dyes in water.
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Eosin Y
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Eosin
Eosin Y
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A quantitative study of azure B-eosin-stained blood cells is reported. The effects of variation in stain formulation and staining technique on the binding of azure B and eosin by acidophilic, basophilic and neutrophilic substrates were measured by scanning microdensitometry. The variables considered were stain concentration (the azure B-eosin concentration ratio was constant), azure B concentration (at constant eosin concentration), eosin concentration (at constant azure B concentration), staining time, buffer pH, metal salt contamination, dye contamination, buffer concentration and fixation time. The last two were the only variables which failed to produce changes in dye uptake. Tentative explanations of the observed effects are advanced.
Eosin
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Eosin Y
Blood Stains
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Eosin
Eosin Y
Bovine serum albumin
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A hematoxylin and eosin procedure is described for staining formalin-fixed tissues embedded in glycolmethacrylate with emphasis on 1.5 micron sections. The staining method utilizes Gill's hematoxylin followed by a buffered eosin Y-phloxine B counterstain. Acidophilic and basophilic components of most stained tissue sections had an intense, sharp and clean appearance when observed by light microscopy.
Eosin
ALIZARIN RED
Stain
Basophilic
Eosin Y
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Citations (5)