Cloning and characterization of spike and floral meristem identity genes in miracle wheat (Triticum turgidum var. mirabile)

The APETALA1(AP1) gene of Arabidopsis thaliana specifies flower meristem identity and is required for normal development of sepals and petals.Driven by the CaMV 35S promoter,plant expression vector PCAP constructed with AP1 was introduced into the genome of Brassica napus by Agrobacterium tumefaciens mediated transformation.The AP1 gene was proved by PCR analysis to have been integrated into genome of transgenic Brassica napus and expressed effectively.The T0 generation of the transgenic plants could flower earlier than the non-transformed plants of Brassica napus.These results showed the crucial function of AP1 in promoting flowering was not species-specific.The genetic improvement of Brassica napus for early maturing could be achieved by genetic engineering effectively.

Sepal

Petal

Source

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Isolation and characterization of FLOWERING LOCUS T subforms and APETALA1 of the subtropical fruit tree Dimocarpus longan

Plant Physiology and Biochemistry (2013)

Patrick Winterhagen Pimsiri Tiyayon Alon Samach M. Hegele J. Wünsche

MADS-box

Ectopic expression

Flowering Locus C

10.1016/j.plaphy.2013.07.013

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Citations (44)

MADS-box genes from perennial ryegrass differentially expressed during transition from vegetative to reproductive growth

Journal of Plant Physiology (2004)

Klaus Petersen Thomas Didion Claus H. Andersen Klaus K. Nielsen

MADS-box

Vegetative reproduction

10.1078/0176-1617-01212

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Citations (65)

Toward functional characterization of Triticum aestivum WFCA-coding sequences

Travis Hoffman

Source

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Citations (0)

Functional Analysis of Three Lily (Lilium longiflorum) APETALA1-like MADS Box Genes in Regulating Floral Transition and Formation

Plant and Cell Physiology (2008)

M.-K. Chen I-C. Lin Chang‐Hsien Yang

Three cDNAs showing a high degree of homology to the SQUA subfamily of MADS box genes were isolated and characterized from the lily (Lilium longiflorum). Lily MADS Box Gene 5 (LMADS5) showed high sequence identity to oil palm (Elaeis guineensis) SQUAMOSA3 (EgSQUA3). LMADS6 is closely related to LMADS5 whereas LMADS7 is more related to DOMADS2, an orchid (Dendrobium) gene in the SQUA subfamily. The expression pattern for these three genes was similar and their RNAs were detected in vegetative stem and inflorescence meristem. LMADS5 and 6 were highly expressed in vegetative leaves and carpel, whereas LMADS7 expression was absent. Ectopic expression of LMADS5, 6 or 7 in transgenic Arabidopsis plants showed novel phenotypes by flowering early and producing terminal flowers. Homeotic conversions of sepals to carpelloid structures and of petal to stamen-like structures were also observed in 35S::LMADS5, 6 or 7 flowers. Ectopic expression of LMADS6 or LMADS7 was able to complement the ap1 flower defect in transgenic Arabidopsis ap1 mutant plants. These results strongly indicated that the function of these three lily genes was involved in flower formation as well as in floral induction. Furthermore, the ability of lily LMADS6 and 7 to complement the Arabidopsis ap1 mutant provided further evidence to show that the conserved motifs (paleoAP1 or euAP1) in the C-terminus of the SQUA/AP1 subfamily of MADS box genes is not strictly necessary for their function.

MADS-box

Sepal

Petal

Antirrhinum majus

Ectopic expression

Lilium

10.1093/pcp/pcn046

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Citations (63)

Overexpression of MdMADS5, an APETALA1-like gene of apple, causes early flowering in transgenic Arabidopsis

Plant Science (2002)

Nobuhiro Kotoda Masato Wada Shinnosuke Kusaba Yuriko Kano‐Murakami Tetsuo Masuda

Antirrhinum

Malus

Antirrhinum majus

Rosette (schizont appearance)

10.1016/s0168-9452(02)00024-9

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Citations (91)

Evolution of Floral Meristem Identity Genes. Analysis ofLolium temulentum Genes Related to APETALA1 andLEAFY of Arabidopsis

PLANT PHYSIOLOGY (2001)

Greg F. W. Gocal Rod W. King Cheryl Blundell Owen Schwartz Claus H. Andersen

Abstract Flowering (inflorescence formation) of the grass Lolium temulentum is strictly regulated, occurring rapidly on exposure to a single long day (LD). During floral induction, L. temulentum differs significantly from dicot species such as Arabidopsis in the expression, at the shoot apex, of twoAPETALA1 (AP1)-like genes, LtMADS1 andLtMADS2, and of L. temulentum LEAFY(LtLFY). As shown by in situ hybridization,LtMADS1 and LtMADS2 are expressed in the vegetative shoot apical meristem, but expression increases strongly within 30 h of LD floral induction. Later in floral development,LtMADS1 and LtMADS2 are expressed within spikelet and floret meristems and in the glume and lemma primordia. It is interesting that LtLFY is detected quite late (about 12 d after LD induction) within the spikelet meristems, glumes, and lemma primordia. These patterns contrast with Arabidopsis, whereLFY and AP1 are consecutively activated early during flower formation. LtMADS2, when expressed in transgenic Arabidopsis plants under the control of theAP1 promoter, could partially complement the organ number defect of the severe ap1-15 mutant allele, confirming a close relationship between LtMADS2 andAP1.

Leafy

Primordium

AP-1 transcription factor

Lemma (botany)

10.1104/pp.125.4.1788

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Citations (132)

Pistillody is caused by alterations to the class-B MADS-box gene expression pattern in alloplasmic wheats

Planta (2004)

Shigeo Takumi Koji Murai Eriko Yoshida Hama Yasunari Ogihara

MADS-box

10.1007/s00425-003-1157-6