Carbohydrate composition of lymphocyte plasma membrane from pig mesenteric lymph node
15
Citation
17
Reference
10
Related Paper
Citation Trend
Abstract:
Pig lymphocyte plasma membrane isolated from mesenteric lymph node contained 69 mug of carbohydrate/mg dry wt., which was made up of neutral sugar, amino sugar and sialic acid in the molar proportions 5:1.7:1. The neutral sugar comprised fucose, ribose, mannose, glucose, galactose and inositol (molar proportions 2:9:11:15:26:1), and the amino sugar glucosamine and galactosamine (molar ratio 2:1). The ribose was most probably derived from RNA. All of the fucose and mannose and almost all of the glucosamine were associated with the membrane protein whereas the membrane lipid contained all of the inositol. The remaining sugars were distributed in various ratios between the protein and lipid fractions.Keywords:
Galactosamine
Ribose
Hexosamines
Neuraminic acid
Galactosamine
Cite
Citations (12)
Hexosamines
Galactosamine
Amino sugar
Cite
Citations (69)
An enzymatic method is described for distinguishing D-glucosamine from galactosamine. The method is based on the fact that glucosamine is phosphorylated by ATP in the presence of hexokinase, whereas galactosamine is not. Application of the procedure for the assay of biological preparations is illustrated by the demonstration of D-glucosamine in hydrolysates of a polysaccharide isolated from Shigella flexneri.
Galactosamine
Hexokinase
Cite
Citations (20)
Galactosamine
Hexosamines
Cite
Citations (10)
Galactosamine
Cite
Citations (15)
Incorporations of radioactive mannose, galactose and fucose into MOPC 104E mouse plasma-cell tumour suspensions suggest a stepwise addition of carbohydrate residues to immunoglobulin M (IgM) during the process of secretion. Mannose and glucosamine residues are added at an early stage, whereas galactose and fucose are added just before, or at the time that, IgM leaves the cell. Free light chains secreted in excess by the same tumour cells incubated with mannose, galactose or fucose contained barely detectable amounts of radioactivity.
Cite
Citations (67)
Galactosamine
Glycogen branching enzyme
Disaccharide
Hexosamines
Cite
Citations (37)
Galactosamine
Hexosamines
Amino sugar
Glycoconjugate
Cite
Citations (244)
The influence of glucosamine, galactosamine, glucuronic acid and of some derivatives of these compounds on mucopolysaccharide metabolism and on protein metabolism of primary fibroblast monolayer cultures was studied. Glucosamine, glucosamine · HC1, glucosamine · HJ and glucosamine sulfate (in concentrations of 100 µg/ml each) caused a considerable, statistically highly significant increase of the secretion of mucopolysaccharides by the fibroblasts. N-Acetylglucosamine and N-acetylgalactosamine were effective too, though to a lesser degree. Glucuronic acid was without any influence on mucopolysaccharide production. The protein content of the cultures was not affected by the 7 compounds mentioned so far in concentrations of up to 100 µg/ml. Galactosamine and betaineglucuronate induced a certain depression of a mucopolysaccharide production and simultaneously a decrease of the cellular protein content.
Galactosamine
Uronic acid
Hexosamines
N-Acetylglucosamine
Cite
Citations (50)
There have been several reports of the incorporation of glucosamine into liver glycogen by an intraperitoneal injection of galactosamine, but it has not previously been considered that glucosamine is a normal component of liver glycogen. We now report that glucosamine occurs endogenously in rabbit- and pig-liver glycogens in the amount of about 1 nmol per 10 mg glycogen. Like the glucosamine incorporated by exogenous administration of galactosamine, the endogenous glucosamine takes the place of 1,4-linked alpha-glucose residues. It is found in both the outer and inner chains of the glycogen molecule.
Galactosamine
Glycogen branching enzyme
Cite
Citations (28)