Effects of Hexosamine Derivatives and Uronic Acid Derivatives on Glycosaminoglycane Metabolism of Fibroblast Cultures
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The influence of glucosamine, galactosamine, glucuronic acid and of some derivatives of these compounds on mucopolysaccharide metabolism and on protein metabolism of primary fibroblast monolayer cultures was studied. Glucosamine, glucosamine · HC1, glucosamine · HJ and glucosamine sulfate (in concentrations of 100 µg/ml each) caused a considerable, statistically highly significant increase of the secretion of mucopolysaccharides by the fibroblasts. N-Acetylglucosamine and N-acetylgalactosamine were effective too, though to a lesser degree. Glucuronic acid was without any influence on mucopolysaccharide production. The protein content of the cultures was not affected by the 7 compounds mentioned so far in concentrations of up to 100 µg/ml. Galactosamine and betaineglucuronate induced a certain depression of a mucopolysaccharide production and simultaneously a decrease of the cellular protein content.Keywords:
Galactosamine
Uronic acid
Hexosamines
N-Acetylglucosamine
Hexosamines
Galactosamine
Uronic acid
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Hexosamines
Galactosamine
Amino sugar
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Uronic acid
Iduronic acid
Tetrasaccharide
Epimer
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Uronic acid
Dermatan sulfate
Carbazole
Chondroitin
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Iduronic acid
Galactosamine
Uronic acid
Hexosamines
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Uronic acid
Galactosamine
Chondroitin
Hexosamines
Fraction (chemistry)
Hyaluronidase
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The influence of glucosamine, galactosamine, glucuronic acid and of some derivatives of these compounds on mucopolysaccharide metabolism and on protein metabolism of primary fibroblast monolayer cultures was studied. Glucosamine, glucosamine · HC1, glucosamine · HJ and glucosamine sulfate (in concentrations of 100 µg/ml each) caused a considerable, statistically highly significant increase of the secretion of mucopolysaccharides by the fibroblasts. N-Acetylglucosamine and N-acetylgalactosamine were effective too, though to a lesser degree. Glucuronic acid was without any influence on mucopolysaccharide production. The protein content of the cultures was not affected by the 7 compounds mentioned so far in concentrations of up to 100 µg/ml. Galactosamine and betaineglucuronate induced a certain depression of a mucopolysaccharide production and simultaneously a decrease of the cellular protein content.
Galactosamine
Uronic acid
Hexosamines
N-Acetylglucosamine
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Bilateral medial meniscectomy of the knee joint was undertaken in 4 mature greyhounds. Four greyhounds of similar age served as nonsurgical controls. Six months after meniscectomy, all animals were sacrificed and femoral head articular cartilage (FHC) examined for collagen, uronic acid, galactosamine, glucosamine and hyaluronic acid (HA) content. The sequential extractability of proteoglycans (PG) with non-dissociative (0.5 M GuHCl) and dissociative (0.4 M GuHCl) solvents was also investigated. The levels of collagen, uronic acid, galactosamine and glucosamine of the FHC of the meniscectomized group remained normal relative to FHC taken from the control greyhounds but the level of HA was significantly depressed (p less than 0.05). The amount of PG extracted by 0.5 M GuHCl from the FHC of the meniscectomized group was 2.5 X that extracted from control cartilage. In addition, subsequent extraction of the residues with 4.0 M GuHCl only released half the amounts of PG extracted from control FHC under the same conditions.
Uronic acid
Galactosamine
Hexosamines
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The effect of exogenous glucosamine and other aminosugars on the incorporation of 3H-leucine 3H-uridine and 3H-thymidine into mitogen induced lymphocytes was studied. D-glucosamine D-galactosamine and D-mannosamine inhibit the incorporation of these precursors into protein. RNA and DNA of blast cells. Glucose and pyruvate did not reverse this inhibition. Glucosamine - 6P, acetylglucosamine and acetylglucosamine and have shown a considerably lower inhibition in the uptake of these labeled precursors.
Galactosamine
Hexosamines
Thymidine
N-Acetylglucosamine
Amino sugar
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Uronic acid
Periodate
Arabinose
Pentose
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