Correlative study between CT features and expression of N-ras gene、C-myc gene in hepatocelluar carcinoma
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Objective:To study the relationship between SCT findings and the expression of N ras gene?C myc gene in hepatocellular carcinoma(HCC).Methods:SCT scan was performed in 36 patients with pathologically proved hepatocellular carcinoma,and the expression of N ras?C myc gene in tumor specimens was estimated with immunohistochemical SABC method.Results:C myc protein positive rate was higher in the group whose diameter is smaller than 5cm and equal to 5cm,and larger than 3cm than that whose diameter is smaller than 3cm and equal to 3cm,and lower in the group cirrhosis of liver(P0.05).Conclusion:This study shows the SCT findings of HCC is related with the expression of the gene,and the SCT imaging of HCC can indicate the expression of C myc gene to some extent.Cite
Objective:To investigate the relation between syndecan-1 protein and malignant phenotypes of hepatocellular carcinoma (HCC). Methods:Thirty-one formalin-fixed sections were obtained. The syndecan-1 was measured with immunohistochemistry assay (ABC method). Results:Among 31 HCC tissues, 18(58.1%) show negative staining; the syndecan-1 negative rate was higher in HCC with poor differentiation than in those well differentiated (80% vs 37.5%, P0.05); in large HCC than in small HCC (85.9% vs 35.3, P0.01); and in HCC with the presence of tumor-cells in blood than in those without. No correlation was found between syndecan-1 expression and serum AFP level, as well as chronic B-type hepatitis and liver cirrhosis. Conclusion:Our study indicated that syndecan-1 expression was correlated with the growth, differentiation, invasiveness, metastasis of HCC. It was possible that syndecan-1 was a negative regulator of these malignant phenotypes of HCC and might be regarded as suppressive gene for tumor.
Syndecan 1
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Objective To investigate the relationship between p53 and c-myc gene expression and the clinicopathological factors of hepatocellular carcinoma and the effect of schistosomiasis on p53,c-myc gene expression.Methods Fifty-eight patients of hepatocellular carcinoma were selected and divided into two groups: HCS group(hepatocellular carcinoma with schistosomiasis,n=23) and HC group(hepatocellular carcinoma without schistosomiasis,n=35).Immunohistochemistry method was used to detect the p53 and c-Myc proteins expression of all the 58 patients.Results The positive rates of p53 protein expression in HCS group and HC group were 73.9%(17/23) and 31.4%(11/35) respectively,and there were significant differences between two groups(P0.01).The positive rates of p53 and c-Myc in the HCC tissue were significantly correlated with the presence of extrahepatic metastasis,the recurrence of tumor and the differentiation of tumor(P0.05 or 0.01).Conclusion There was higher gene mutation rate in the patients of hepatocellular carcinoma complicated with schistosomiasis,which suggests that schistosomiasis can promote p53 mutation proteins over-expression in the histiocytes of HCC.The above evidences indicate that detecting the expressions of p53 and c-Myc could be considered as reference indexs on evaluating biological behavior of HCC.
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Hematology
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Objective:To Study the expressions of C-myc and p16 in colorectal carcinoma and their correlation.Methods:The expressions of C-myc and p16 in 78 colorectal carcinoma, 20 juxtacancerous tissues and 20 adenoma of colon were de tected by immunohistochemistry. Then the correlation of them was analyzed. Results:The positive-expression rate of C-myc was the highest, and the negative-ex pression rate of p16 was the lowest in colorectal carcinoma(P0 .05). The positive-expression of C-myc and the negative-expression of p16 w ere closely related to clinical staging, degree of differentiation, lymph-node metastasis and distant metastasis of colorectal carcinoma(P0.0 5). There was a relationship between C-myc and p16 (P0.05). Conclusion:C-myc was in coordination with p16. The up-regulated C-myc and inact ivated p16 should play a crucial role in the development of colorectal carcinoma.
Colon carcinoma
Negative correlation
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The gene expression profile p16, c-erbB-3 and bcl2 in hepatocellular carcinoma (HCC) patients with and without associated HCV infection, was assessed. Forty-eight subjects were included in the study and divided equally into two groups: HCC with and without HCV associated infection. Adjacent paracancerous tissues were assessed as control samples. Correlations with various clinico-pathological parameters of the tumour were assessed: stage, grade, and tumour size. The c-erbB-3 oncogene was expressed in 83.33% (40/48) of the total HCC sample and in 31.25% (15/48) of the noncancerous lesions. C-erbB-3 was expressed in 87.5% (21/24) of the HCC cases with associated HCV infection and in 79.16% (19/24) of the HCC cases without associated HCV infection. Gene expression of c-erbB-3 was significantly correlated with the clinico-pathological parameters of the tumour. P16 gene expression was found in 12.5% (6/48) of the total HCC sample and in 25% (12/48) of the para-cancerous lesions. P16 was expressed in 12.5% (3/24) of HCC cases with and without associated HCV infection. Gene expression of p16 exhibited significant negative correlation with clinico-pathological parameters of the tumour. Bcl2 gene expression was found in 20.8% (10/48) of the total HCC sample and in the para-cancerous lesions. Bcl2 was expressed in 20.8% (5/24) of the HCC cases with and without HCV associated infection. Gene expression of bcl2 did not show significant correlations with the clinico-pathological parameters of the tumour. In conclusion, gene expression profiles of p16 and c-erbB-3 could be used as prognostic molecular markers in HCC.
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Objective To study the relationship between the expression of nm23 H1 gene,C erbB 2 gene and SCT findings in hepatocellular carcinoma (HCC).Method SCT scan was performed in 36 patients with pathologically proved hepatocellular carcinoma,and the expression of nm23 H1,C erbB 2 gene in tumor specimens was estimated with immunohistochemical SABC method.Result nm23 H1 protein positive rate of HCC specimens was 58.3%,nm23H1 protein positive rate was lower in high potential invasion and metastasis group (intrahepatic daughter foci,tumor emboli in portal veins,lymphaden metastasis).C erbB 2 protein positive rate of HCC specimens was 71.1%,C erbB 2 protein positive rate was higher in the group whose diameter is smaller than 5cm and equal to 5cm,and larger than 3cm than that whose diameter is smaller than 3cm and equal to 3cm,and was higher than that whose diameter is larger than 5cm.Conclusion This study shows the SCT findings of HCC is related with the expression of nm23 H1 and C erbB 2 gene.
Metastasis Suppressor Gene
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Aim: To study the expression of P16 in tissues of primary hepatocellular carcinoma (HCC). Methods: The expression P16 in 43 HCC and 26 paracancerous tissues were detected by immunohistochemistry. Results: Deletion rate of P16 in HCC was significantly higher than that in paracancerous tissues (65.1% vs.11.5%,P0.01). The deletion rate oi P16 in grade Ⅲ was also significantly higher than that in grades Ⅰ and Ⅱ (both P 0.05). Conclusion: Deletion of P16 expression is closely related to the carcinogenesis of HCC. The low differentiated HCC is correlated to higher deletion rate of P16.
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Liver Cancer
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Objective:To approach ras and p16 protein expression in hepatocellular carcinoma .To investigate their effect on recurrence and prognosis of hepatocellular carcinoma after hepatectomy.Methoeds:Ras and p16 protein expression in 53 cases of human hepatocellular carcinoma and in 12 cases of normal liver were examined by using immunohistochemical technique,respectively.Results:(1)Positive staining rate of ras in hepatocellular carcinoma and in normal liver was 66.0% and 34.0%(P0.05),respectively.The positive expression of ras correlated with tumor size,intrahepatic metastsis,recurrence and survical rate after hepatectomy(P0.05).(2)Positive staining rate of p16 was more higher in normal liver than in hepatocellular carcinoma(P0.01).The negative expression of p16 correlated with phthological grading ,intrahepatic metastasis and prognosis(P0.05).(3)There was a significant correlation between ras and p16 protein expression (P0.01).The 3-year recurrence and survival rate was 35.7% and 71.4% respectively in the opposites.Conclusions: Protein expressions of ras and p16 are effective in dicators for recurrence and prognosis of hepatocellular carcinoma.The analysis of multi-gene is more valuable than that of single-gene. [
Grading (engineering)
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