CLINICAL SIGNIFICANCE OF RAS AND P16 PROTEIN EXPRESSION IN HEPATOCELLULAR CARCINOMA
0
Citation
0
Reference
20
Related Paper
Abstract:
Objective:To approach ras and p16 protein expression in hepatocellular carcinoma .To investigate their effect on recurrence and prognosis of hepatocellular carcinoma after hepatectomy.Methoeds:Ras and p16 protein expression in 53 cases of human hepatocellular carcinoma and in 12 cases of normal liver were examined by using immunohistochemical technique,respectively.Results:(1)Positive staining rate of ras in hepatocellular carcinoma and in normal liver was 66.0% and 34.0%(P0.05),respectively.The positive expression of ras correlated with tumor size,intrahepatic metastsis,recurrence and survical rate after hepatectomy(P0.05).(2)Positive staining rate of p16 was more higher in normal liver than in hepatocellular carcinoma(P0.01).The negative expression of p16 correlated with phthological grading ,intrahepatic metastasis and prognosis(P0.05).(3)There was a significant correlation between ras and p16 protein expression (P0.01).The 3-year recurrence and survival rate was 35.7% and 71.4% respectively in the opposites.Conclusions: Protein expressions of ras and p16 are effective in dicators for recurrence and prognosis of hepatocellular carcinoma.The analysis of multi-gene is more valuable than that of single-gene. [Keywords:
Grading (engineering)
Cite
Objective To investigate the expression and clinical significance of RUNX3,p21CIP1/WAF1,cyclinD1 protein in human hepatocellular carcinoma(HCC).Methods Western Blot was used to detect RUNX3,p21CIP1/WAF1,cyclinD1 protein expression level in samples of hepatocellular carcinoma and corresponding para-cancer tissue,which were obtained from surgically resection specimens of 43 patients.Results Western Blot showed that the expression of RUNX3 and p21CIP1/WAF1 in hepatocellular carcinoma was significantly lower than those in corresponding paracancer tissue(P0.05) and the expression of cyclinD1 in hepatocellular carcinoma was significantly higher than that in corresponding para-cancer tissue(P0.05).The depression of RUNX3 was correlated with TNM stage and pathologic differentiation degree(P0.05).CyclinD1 and p21CIP1/WAF1expression was significantly associated with TNM stage and pathologic differentiation degree(P0.05).The correlationship between RUNX3 and p21CIP1/WAF1 expression was significant(r=0.696,P0.5) and there was a significant inverse correlationship between RUNX3 and cyclinD1 expression(r=-0.435,P0.05).Conclusion The abnormal expression of RUNX3,p21CIP1/WAF1,cyclinD1 gene in human hepatocellular carcinoma suggest that RUNX3,p21CIP1/WAF1,cyclinD1 gene may play an important roles in the development and progression of hepatocellular carcinoma.The combination detection of the three indices may provide reference to the diagnosis and evaluating the clinicopathological parameters of the hepatocellular carcinoma.
Clinical Significance
Liver Cancer
Cite
Citations (0)
Overexpression of p53 in hepatocellular carcinomas: A clinicopathological and prognostic correlation
Abstract Overexpression of the p53 tumour suppressor gene is one of the most common abnormalities in primary human cancers and appears to be a result of point mutation within a highly conserved region of the gene with subsequent encoding for a mutant, more stable protein. In this study, 71 surgically resected hepatocellular carcinomas (HCC) were examined to study the expression of the p53 gene, its relation with clinicopathological parameters and its prognostic significance. Using immunohistochemical detection for mutant p53 protein with monoclonal antibody PAb1801, p53 overexpression was found in 22 tumours (31%) but in none of the non‐tumorous liver specimens. Overexpression of p53 was more frequent in tumours with poor cellular differentiation ( P = 0.01), in tumours > 5 cm in diameter ( P = 0.05), and in those with giant cells present ( P = 0.03) and, less significantly, of massive type of Eggel's classification ( P = 0.06). It did not have any significant correlation with hepatitis B or C status, background liver disease or serum α‐fetoprotein levels, nor was it related to tumour invasiveness (venous permeation, direct liver invasion and tumour microsatellite formation). In addition, the presence of p53 mutant protein did not influence tumour recurrence or patients’ survival rates. The data suggested that p53 mutation in HCC was associated with a later stage of oncogenesis. However, it was not apparently related to tumour invasiveness/aggressiveness and prognosis.
Cite
Citations (45)
Objective To investigate the expression of connexin43 and E-cadherin in hepatocellular carcinoma and their relationship with clinicopathologic parameter. Methods The expression of connexin43 and E-cadherin were studied in 47 cases of hepatocellular carcinoma by using streptavidin peroxidase immunohistochemistry. Results The positive rate of Cx43 and E-cadherin in the HCC was 42.55 % and 46.81 %, respectively. With the progression of hepatocellular carcinoma course, the positive rate of Cx43 was decreased (P= 0.006 ). Cx43 positive rate was lower in the group with cirrhosis background than that in the group without cirrhosis background (χ2= 4.7135 , P= 0.03 ). In comparision with the group of intrahepatic vascular embolism, E-cadherin positive rate was apparently decreased in the group without intrahepatic vascular embolism (P= 0.028 ). No significant association were found between the expression of Cx43, E-cadherin and tumor size, the extent of differentiation. Spearman correlation analysis revealed that the expression of Cx43 and E-cadherin had no significant relationship. Conclusion The aberrant expression of Cx43 and E-cadherin may play a role in occurrence, progression of hepatocellular carcinoma. Detecting the expression of Cx43 and E-cadherin may be contribute to synthetically evaluating the biological behavior of hepatocellular carcinoma.
Cite
Citations (0)
Objective: To investigate the expression and clinical significance of gene of phosphate and tension homology deleted on chromsome ten( PTEN) and p53 in hepatocellular carcinoma( HCC) sample. Methods: The protein levels of PTEN and p53 were examined by EliVision immunohistochemistry in 70 samples of HCC tissue and 40 samples of adjacent hepatic tissue,while the correlation between the expression and clinicopathological features was analyzed. Results: The positive expression rate of PTEN in HCC tissue was 57. 1%,significantly lower than that in adjacent hepatic tissue,which the positive expression rate was 95. 0%( P 0. 01). Patients with advanced stage of tumor or lymph node metastasis had a lower tendency to express PTEN in HCC tissue( P 0. 01). The positive expression rate of p53 was 60. 0% in HCC tissue,significantly higher than that in adjacent hepatic tissue,which the positive expression rate was 15. 0%( P 0. 01). There were no statistical significance for the p53 expression in HCC tissue in clinicopathological parameters such as tumor size,pathologic grade,TNM stage and lymphnode metastasis( P 0. 05). The expression of PTEN was negatively related with that of p53 in HCC tissue( P 0. 05). Conclusions: The expression of PTEN may be associated with the progression of HCC. It may serve as an index for prognosis of HCC.
Clinical Significance
Cite
Citations (0)
Liver metastases are the most critical prognostic factors for patients with colorectal carcinomas (CRC). It has been reported that the dysregulation of hepatocyte nuclear factor‐4α (HNF4α) expression is linked to the development of CRC, gastric cancer and hepatocellular carcinoma. The purpose of the present paper was to examine the P1 and P2 promoter‐driven HNF4α (P1 and P2) expression in surgically resected CRC. Immunohistochemically, P1, P2, MUC1 and CD10 expression were evaluated in 63 cases of primary CRC. Positive staining with P1, P2, MUC1 and CD10 antibodies were observed in 37 (59%), 63 (100%), 42 (67%) and 27 (43%) cases, respectively. Loss or decreased P1 expression was observed with respect to the depth of the tumor invasion. The frequency of P1‐positive expression in Dukes' C and D tumors was significantly lower than that in Dukes' A and B tumors. There was a relationship between the loss of P1 expression and metachronous liver metastases, and the survival rate of the P1‐negative patients without liver metastasis at the time of the primary CRC resection tended to be worse than that of the P1‐positive patients. These findings suggest that downregulation of P1 expression is involved in tumor metastasis and a worse prognosis.
Cite
Citations (39)
BACKGROUND: This research was aimed to study the expression of Serine/arginine rich splicing factor 2 (SRSF2) in tissues of hepatocellular carcinoma, and explore the relationship between the expression and the clinic pathological and prognosis of human hepatocellular carcinoma (HCC). METHODS: One h undred and fifty-three pairs HCC tissues and adjacent normal tissue were collected from January 2010 to March 2013. The expression of SRSF2 gene was detected by immunohistochemistry, western blotting and real-time quantitative polymerase chain reaction (PCR), and the relationship between the expression and the clinic pathological and prognosis of HCC being analyzed. RESULTS: In 153 cases of hepatocellular carcinoma, SRSF2 was highly expressed in 93 cases, low expression of 60 cases, immunohistochemistry score (6.50 ± 2.82), which was significantly higher than that in adjacent normal tissues (2.94 ± 1.23) (P< 0.05). The expression of SRSF2 in HCC was not associated with gender (χ2= 0.014, P= 0.906), age (χ2= 0.007, P= 0.931), tumor size (χ2= 3.566, P= 0.059) and T stage (χ2= 2.708, P= 0.100), and was significantly correlated with tumor differentiation (χ2= 9.687, P= 0.007), lymph node metastasis (χ2= 4.827, P= 0.028), distal metastasis (χ2= 9.235, P= 0.002), tumor, node, metastasis (TNM) stage (χ2= 3.978, P= 0.046), portal vein invasion and serum alpha-fetoprotein (χ2= 14.919, P= 0.000). The expression of SRSF2 protein in hepatocellular carcinoma was positively correlated (r = 0.704, P< 0.05) with serum alpha-fetoprotein through Pearson analysis. The survival rates of SRSF2 overexpressing hepatocellular carcinoma were 74.19%, 44.09%, 26.88%, 24.73% and 21.51% at 1 year, 2 years, 3 years, 4 years and 5 years respectively, which were lower than those of SRSF2 low expression group (93.33%, 71.67%, 56.67%, 51.67% and 50.00%). CONCLUSION: SRSF2 is highly expressed in hepatocellular carcinoma and its expression increases with the degree of tumor differentiation and TNM staging. It is related to lymph node metastasis and metastasis of tumor cells, and is positively related to serum alpha fetoprotein content, and affects the postoperative survival time of HCC patients.
Splicing factor
T-stage
Cite
Citations (7)
Objective:To study the status and significances of bcl 2,p53 and p21 gene expression in hepatocellular carcinoma(HCC).Methods:The samples resected from 55 cases of hepatocellular carcinoma and nodular liver cirrhosis were detected with the antibodies against bcl 2,p53 and p21 in the S P immunohistochemistry study.The relationships between the expression rate and pathological grades and prognosis were analyzed.Results:The positive expression rate of bcl 2 gene protein in HCC and nodular liver cirrhosis were 72.7%(40/55) and 36.4%(20/55) respectively,both present disparity( P 0.05).The positive expression rate of p53 protein in HCC was 54.5%(30/55),the negative expression of all nodular liver cirrhosis tissues was found,both present striking disparity ( P 0.01).The positive expression rate of p21 protein in the HCC and nodular liver cirrhosis were 63.6%(35/55) and 38.2% (21/55) respectively,both not present disparity ( P 0.05).The expression rate and expression intensity of bcl 2、p53 and p21 was not associated with pathological grades in the HCC ( P 0.05). In addition,together expression rate of bcl 2、p53 and p21 in the follow up patients (16 cases) was 50%(8/16).Conclusion:The results suggest that the occurrence of HCC has a close relation with the over expression and mutation of bcl 2、p53 and p21 gene proteins.There may be a joint action among these genes.Because the expression of bcl 2、p53 and p21 can reflect the differential and proliferative degrees of tumour cells,it can be used as the indicators in the diagnosis and prognosis of HCC.
Cite
Citations (0)
Objective:To study the clinical significance of Caveolin-1(Cav-1)in hepatocellular carcinoma.Methods:One hundred and sixty cases of hepatocellular carcinoma were tested for expression of Cav-1 by SP immunohistochemistry.Results: Out of 160 HCC samples,65(41%)were negative for Caveolin-1,95(59%) were positive.The Cav-1-positive cells in HCC tissues showed unequivocal cytoplasmic staining pattern.The positive rate of Cav-1 expression was significantly correlated with histological differentiation,portal venous invasion,intrahepatic metastasis and recurrence(P0.05).K-M survival curve showed the survival rate was significantly lower in the positive expression of the Cav-1 patients compared to the negative ones(P0.001).The Multivariate COX proportional hazards model analysis also showed that the expression of Cav-1 was an independent predictor of overall survival(P=0.047).Conclusion: Cav-1 may play an important role in the development and metastasis of hepatocellular cancer.
Clinical Significance
Caveolin 1
Cite
Citations (1)
Hepatocellular carcinoma (HCC) is one of the most frequent cancers worldwide with a high mortality. Immunohistochemical overexpression of the p53 protein was correlated with a poor prognosis in various human malignancies, including HCC. In our study, 45 resected HCCs were examined to evaluate the expression of p53 and its correlation with clinicopathological parameters. Immunohistochemical detection of p53 with monoclonal human antibody, revealed its overexpression in 20 tumors (44%), including diffuse positive in 7 cases (35%), heterogeneous in 5 (25%), and focal in 8 (40%). We considered a positive reaction only in the presence of immunostained nuclei in brown shades in more than 5% of the tumor nuclei. To elucidate the significance of p53 in HCC, we correlated its protein expression with major clinicopathological features. We did not observe significant correlation with sex, age, presence of cirrhosis, chronic hepatitis status, tumoral necrosis and tumor size. The density and intensity of p53 revealed significant correlation with histological grade (P=0.008 and P=0.014) and tumor stage (P=0.005 and P=0.007). In conclusion, our results suggest that overexpression of p53 is associated with HCC progression and contributes to disease progression. Moreover, p53 expression may be a valuable marker of HCC prognosis.
HCCS
Clinical Significance
Tumor progression
Cite
Citations (1)
AIM To study the expression of PTEN protein in hepatocellular carcinoma (HCC) and its significance in clinical pathology. METHODS Fifty six cases of hepatocellular carcinoma, 17 cases of liver cirrhosis and 3 cases of normal liver tissues were stained with PTEN protein rabbit antibody by immunohistochemical method. The results were analysed with clinical pathology. RESULTS Of the 56 cases of hepatocellular carcinoma, 71% (40/56) were PTEN protein positive. And the lowest differential HCC showed the lowest positive rate 57% (12/21). A significant difference ( P 0.05) was observed between G 1, G 2 and G 3 grades of HCC in the expression of PTEN protein. Of the 17 cases of liver cirrhosis, 94% (16/17) were PTEN protein positive. Significant difference ( P 0.05) was also observed in HCC and liver cirrhosis. High degree loss of PTEN protein was observed in HCC, and the lowest differential HCC showed the lowest positive rate. CONCLUSION Loss of PTEN protein expression might play a certain role in the progression of HCC and patient outcome.
Clinical Significance
Cite
Citations (0)