X-ray Holographic Microscopy by Self-Interference Incoherent Digital Holography
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New approach to full-field soft x-ray holographic microscopy is being developed based on the principle of self-interferencce incoherent digital holography, with simple optical configurations and higher efficiencies. Theorectial and preliminary experimental results will be presented.Keywords:
Digital Holographic Microscopy
Digital holography
Interference microscopy
We present recent progress in the development of self-interference incoherent digital holography for holographic fluorescence microscopy, including aberration compensation of fluorescent holographic image and 3D differential holography.
Digital Holographic Microscopy
Digital holography
Holographic interferometry
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Digital and analog holography, along with its many variations, viz., holographic interferometry, holographic microscopy, holographic tomography, multiwavelength digital holography, phase-shifting holography, compressive holography, coherence holography, etc., have become the methods of choice for various metrological applications in three-dimensional (3D) imaging. In this review, we discuss the basic principles of analog and digital holography and the various topics mentioned above, with selected applications to real-world problems. We also discuss other related topics such as dynamic holography, non-Bragg orders, and compressive holographic tomography, nonlinear holography, holographic TV, as well as a nonholographic technique for 3D visualization, viz., transport of intensity imaging. Finally, we expose interested readers to contemporary topics in the area, viz., nonlinear holography and real-time holographic TV.
Digital holography
Digital Holographic Microscopy
Holographic interferometry
Holographic display
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A telecentric in-line-and-off-axis hybrid digital holography reconstruction method is proposed to enable high-resolution microscopic quantitative imaging. The experiment of red blood cells was conducted to demonstrate the validity of the proposed approach.
Digital Holographic Microscopy
Digital holography
Line (geometry)
Phase imaging
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Theory and method of reconstruction of digital holographic microscopy are discussed.A magnified image of USAF 1950 is recorded with off-axis digital holographic setup.The holograms are numerically reconstructed in amplitude and phase distribution.The digital reconstruction algorithm based on Fresnel diffraction is analyzed and applied.The modeling of digital reference wave for digital reconstruction,the removing of the zero-order and the twin images by frequency filter and reconstruction parameters are solved.A focused image of the specimen is reconstructed to show that this technology can be used in phase-contrast measurements of micro-object.
Digital Holographic Microscopy
Digital holography
3D Reconstruction
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Digital holography enables a multifocus quantitative phase microscopy for the investigation of reflective surfaces and for marker-free live cell imaging. For digital holographic long-term investigations of living cells an automated (subsequent) robust and reliable numerical focus adjustment is of particular importance. Four numerical methods for the determination of the optimal focus position in the numerical reconstruction and propagation of the complex object waves of pure phase objects are characterized, compared, and adapted to the requirements of digital holographic microscopy. Results from investigations of an engineered surface and human pancreas tumor cells demonstrate the applicability of Fourier-weighting- and gradient-operator-based methods for robust and reliable automated subsequent numerical digital holographic focusing.
Digital Holographic Microscopy
Digital holography
Holographic interferometry
Digital Imaging
Live cell imaging
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Polychromatic digital holographic microscopy (P-DHM) has proven its capacity to provide quasi-coherent noise-free quantitative-phase images, allowing a high-quality visualization of cell structure. In this work we propose a fully automated hologram reconstruction methodology, including a fast-numerical approach for the correction of the defocusing resulting from both the axial chromatic aberrations as well as the fluctuations of the optomechanical elements. This methodology able to reconstruct a large number of holograms paves the way to develop a time-resolved P-DHM capable of non-invasively visualizing both the fine cell structure and dynamics.
Digital Holographic Microscopy
Digital holography
Computer-Generated Holography
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New approach to full-field soft x-ray holographic microscopy is being developed based on the principle of self-interferencce incoherent digital holography, with simple optical configurations and higher efficiencies. Theorectial and preliminary experimental results will be presented.
Digital Holographic Microscopy
Digital holography
Interference microscopy
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Two schemes for digital holographic microscopy are explored. The goal is to revisit the issues that have hindered holographic microscopy since its earlier days, and find ways to overcome these difficulties. The first scheme discussed is a full-field holographic method that takes advantages of the properties of charge-coupled-device detectors, namely their high spatial as well as temporal bandwidths. The second scheme is based ona scanning holographic technique, and is shown to offer a wide range of possibilities such as enhanced transverse resolution, extended depth of focus, or axial sectioning. Both schemes are described, and some of their attributes and relative merits are discussed.
Digital Holographic Microscopy
Depth of field
Digital holography
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Two-dimensional latent images as well as three-dimensional images of finger print molds are produced using quantitative phase microscopy by digital holography, digital interference holography, and low-coherence interference microscopy.
Digital Holographic Microscopy
Digital holography
Interference microscopy
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We present the development of a holographic optical element that creates a micrometrical point source producing the divergent spherical illumination needed for Digital Lensless Holographic Microscopy. Our proposal has been validated with biological samples.
Digital Holographic Microscopy
Digital holography
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