Quantitative phase microscopy imaging of cells using telecentric in-line-and-off-axis hybrid digital holography
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A telecentric in-line-and-off-axis hybrid digital holography reconstruction method is proposed to enable high-resolution microscopic quantitative imaging. The experiment of red blood cells was conducted to demonstrate the validity of the proposed approach.Keywords:
Digital Holographic Microscopy
Digital holography
Line (geometry)
Phase imaging
We present recent progress in the development of self-interference incoherent digital holography for holographic fluorescence microscopy, including aberration compensation of fluorescent holographic image and 3D differential holography.
Digital Holographic Microscopy
Digital holography
Holographic interferometry
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Quantitative phase microscopy (QPM) can be obtained by adding the concept of digital Lateral Shear Interferometry (LSI) to Digital Holography (DH). We demonstrate that the proposed approach offer some important advantages compared to other methods.
Digital Holographic Microscopy
Shearing (physics)
Digital holography
Phase imaging
Holographic interferometry
Phase Unwrapping
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We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.
Digital Holographic Microscopy
Digital holography
Phase imaging
Second-harmonic imaging microscopy
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Citations (45)
A telecentric in-line-and-off-axis hybrid digital holography reconstruction method is proposed to enable high-resolution microscopic quantitative imaging. The experiment of red blood cells was conducted to demonstrate the validity of the proposed approach.
Digital Holographic Microscopy
Digital holography
Line (geometry)
Phase imaging
Cite
Citations (0)
Digital holographic microscopy (DHM) is a quantitative phase imaging (QPI) modality, which retrieves 3D object phase information. The quantification of subcellular features within the object is possible. Its single-shot hologram recording feature makes it suitable for real-time imaging applications. This paper discusses QPI capability of LED-based digital inline holographic microscopy (LDHM), which has gained much attention for its portability, cost-effective features. However, the twin image artifact is present in the inline setup. Though several twin image reduction and elimination methods are developed, the exact phase quantification is always a challenge. Original phase information may be lost after elimination of twin image. There is always a trade-off between twin-image elimination and QPI of inline microscopy setup. This paper discusses the QPI capability of LDHM in comparison with the conventional off-axis DHM. Further, the results of phase objects using both the methods are studied.
Digital Holographic Microscopy
Digital holography
Phase imaging
Digital Imaging
Artifact (error)
Software portability
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Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or technical microstructures, merging beneficial features identified with microscopy, interferometry, holography, and numerical computations. This roadmap article reviews several digital holography-based QPI approaches developed by prominent research groups. It also briefly discusses the present and future perspectives of 2D and 3D QPI research based on digital holographic microscopy, holographic tomography, and their applications.
Digital Holographic Microscopy
Digital holography
Phase imaging
Holographic interferometry
Digital Imaging
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Citations (58)
A dual-wavelength common-path digital holographic microscopy is presented to achieve quantitative phase imaging, which is based on introduction of two fiber-coupled lasers and the reflection from front and back surface of a glass plate.
Digital Holographic Microscopy
Digital holography
Phase imaging
Reflection
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Six different methods for phase compensation in Digital Holographic Microscopy are compared using a calibrated test target and a Toxocara canis larva sample regarding processing time, measurement accuracy, and usefulness in biological imaging.
Digital Holographic Microscopy
Digital holography
Phase imaging
Biological specimen
Holographic interferometry
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We present the imaging of the temporal change of three dimensional movement and µm order structure in the microscopic area by using parallel phase shifting digital holography. The effectiveness of the technique was verified by an experiment.
Digital Holographic Microscopy
Digital holography
Phase imaging
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In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.
Digital Holographic Microscopy
Digital holography
Holographic interferometry
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Citations (1)