Effect of prolonged staining with hematoxylin on detecting Helicobacter pyiori in hematoxylin‐eosin‐stained gastric mucosa
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Abstract:
Helicohacter pylorl (H. pylorl ) Is ldentlflable in hematoxyiin‐eosin (HE)‐stalned preparations, particuularly under a prolonged hematoxylln staining condition. The stalnlng intensity of H. pylori bodies was Increased when the hemetoxylln stalnlng period was doubled from 5 min, the routine period of Meyer's hematoxyiin stalnlng, to 10 min. The stalning intenstty of the background eplthellal nuclei was only mildly increased. Hematoxylin‐eosin staining employing a prolonged hematoxylin staining period gave detedablllty of H. pylori comparable to May‐Glemsa and lmmunoperoxldase staining. The modified HE method is thus Very useful and practical for identifying H. pylori in routine gastric biopsy specimens.Keywords:
Gastric biopsy
Eosin
Reinke crystalloid (CR)s are rod or corn shaped structures present in the interstitial cells (Leydig cell) of human testis. Due to existing controversies and because of their importance in histological studies, we decided to reevaluate their staining behavior with various dyes. Earlier it has been shown that hematoxylin and eosin dyes do not stain CRs even though their protein nature remains undisputed. In the present study, sections of testicular tissues embedded in glycol-methacrylate (GMA) showed that hematoxylin binds to CRs non-specifically and eosin stains them specifically. The reasons for stainability and/or non stainability of CRs with hematoxylin, eosin and other dyes are discussed.
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Eosin Y
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Instead of two-stage staining with hematoxylin-eosin and hallocyanin-picrofuchsin a technique for simultaneous staining in mixtures of hallocyanin and eosin or picrofuchsin is suggested. The stains-cocktails are well preserved for 4-5 weeks and may be used repeatedly.
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Eosin Y
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A standardized thionin-eosinic acid stain was developed as a quick and highly reproducible staining method for bronchial cytology. Bronchial smears and paraffin-embedded sputum samples were stained with thionin-eosin and with the conventional hematoxylin-eosin Y. Spectral absorption characteristics and staining intensity of thionin-eosin-stained cells were investigated by means of cytophotometry. The staining pattern of thionin-eosin is very close to that of the hematoxylin-eosin stain; the contrast between nucleus and cytoplasm is significantly higher for thionin-eosin. Thionin-eosin can be used for "dye-fixation" of cytologic smears and tissue imprints. Blueing and differentiation (as for hematoxylin-eosin) is not required for thionin-eosin; thus, fixation and staining can be performed within two minutes. The spectral absorption characteristics of thionin-eosin allow reliable automated cytophotometric discrimination of cell nuclei and cytoplasm. The standardized thionin-eosin stain is recommended as a substitute for the hematoxylin and eosin stain in bronchial cytology.
Eosin
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Eosin Y
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Eosin
Osmium tetroxide
Eosin Y
Glutaraldehyde
Haematoxylin
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Deparaffinized kidney sections from mice infected withCandida albicans and lung sections from mice infected withBlastomyces dermatitides were stained with the stilbene derivative, Uvitex 2B (1%), and counterstained with haemalum and eosin. Fungi selectively stained with Uvitex 2B are visualized by blue fluorescence under incident illumination with ultraviolet light. Simultaneous or consecutive illumination with transmitted light permits the assignment of fluorescent fungi to haemalum-eosin-stained structures in the section. The most practical means of achieving a high optical contrast with Uvitex 2B in sections and good haemalum-eosin staining is to use the established haemalum-eosin technique, but with a solution containing both 1% eosin and 1% Uvitex 2B in place of eosin alone. Since Uvitex 2B stains all fungi investigated so far, it affords a simple, sensitive and inexpensive method of selectively detecting opportunistic fungal infections in conventional histopathology.
Eosin
Eosin Y
Histopathology
Haematoxylin
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배경: 조직표본제작 시 H&E(Hematoxylin and Eosin)염색은 조직의 형태학적 구조와 세포의 변화 정도를 판단할 수 있는 가장 일반적인 염색방법이다. 이 염색에 사용되는 Hematoxylin과 Eosin은 염료분자와 조직성분의 이온결합 기전에 의해 세포의 핵과 세포질에 각각 염색되며, 두 염료의 pH 변화에 따라 염색성이 달라질 수 있다. 따라서 본원에서 시행하고 있는 H&E 염색방법을 통해 pH 변화에 따른 조직표본의 염색성을 비교하고, 염료의 적정 pH와 pH 유지의 중요성을 알아보고자 한다. 방법: 본 실험은 Liver, Kidney, Lung 의 조직표본을 대상으로 실시하였다. Harris hematoxylin(영동제약, korea) 과 제조한 Eosin(Sigma Chemical, USA)을 사용하여 varistain XY(Shandon, USA)로 염색을 실시하였고 pH는 300 A digital pH meter(Bantex, USA)를 이용하여 측정하였다. pH에 따른 H&E염색의 염색성을 관찰하기 위하여 Harris hematoxylin과 Eosin 염료의 pH를 각각 8군(pH1.5~8.5)으로 나누어 염색을 실시하고, 광학현미경을 이용하여 염색성을 비교하였다. 결과: Harris hematoxylin의 경우 pH1.5, pH4.5에서 염색성이 저하되었고, pH2.5와 pH3.5에서 적정한 염색성을 보였으며, pH5.5~pH8.5에서 염색이 되지 않았다. Eosin의 경우 pH1.5, pH2.5, pH6.5에서 염색성이 저하되었고, pH3.5, pH4.5, pH5.5에서 적정한 염색성을 보였으며. pH7.5, pH8.5에서는 염색이 되지 않았다. 고찰: H&E염색은 pH의 변화에 따라 핵과 세포질의 염색성에 많은 차이를 보인다. 따라서 H&E염색의 염색성은 pH 변화에 영향을 받으므로 pH의 유지가 요구되며, 일정한 염색성을 가진 조직표본을 얻기 위해서는 검사실 실정에 맞는 지속적인 염료의 pH 관리가 필요하다고 사료된다.
Eosin
Eosin Y
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Hematoxylin and eosin staining is the globally accepted staining technique for histology and histopathology sections. Hematoxylin is a natural dye as compared to eosin which is a synthetic dye manufactured from chemicals. Eosin acts as a counterstain to hematoxylin giving a sharp contrast to its blue color. Eosin being a chemical, can cause health hazards and environmental pollution. Using eco-friendly materials and going organic is the demand of this era. Curcuma longa rhizomes are proven to contain curcuminoids that exhibit various medicinal properties and curcumin the bright yellow component of the plant, that is, being used as a colorant. This natural dye from C. longa provides a significant alternative to eosin as they are safer to use without health hazards and are biodegradable and have easy disposability.To evaluate and compare the staining qualities of C. longa (turmeric) with hematoxylin and eosin (H&E).A total of 100 paraffin-embedded tissue block specimens of various pathological tissues were stained with H and E and hematoxylin and curcumin (H&C). Evaluation and comparison of the staining efficacy were done for all the pathological tissues. The obtained data were subjected to statistical analysis.H&C staining gave comparable results with H and E staining with a statistically insignificant P value (p > 0.05).Curcumin has shown equivalent staining to that of eosin. Thus, it can be used as a better alternative.
Eosin
Eosin Y
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Helicohacter pylorl (H. pylorl ) Is ldentlflable in hematoxyiin‐eosin (HE)‐stalned preparations, particuularly under a prolonged hematoxylln staining condition. The stalnlng intensity of H. pylori bodies was Increased when the hemetoxylln stalnlng period was doubled from 5 min, the routine period of Meyer's hematoxyiin stalnlng, to 10 min. The stalning intenstty of the background eplthellal nuclei was only mildly increased. Hematoxylin‐eosin staining employing a prolonged hematoxylin staining period gave detedablllty of H. pylori comparable to May‐Glemsa and lmmunoperoxldase staining. The modified HE method is thus Very useful and practical for identifying H. pylori in routine gastric biopsy specimens.
Gastric biopsy
Eosin
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A hematoxylin and eosin procedure is described for staining formalin-fixed tissues embedded in glycolmethacrylate with emphasis on 1.5 micron sections. The staining method utilizes Gill's hematoxylin followed by a buffered eosin Y-phloxine B counterstain. Acidophilic and basophilic components of most stained tissue sections had an intense, sharp and clean appearance when observed by light microscopy.
Eosin
ALIZARIN RED
Stain
Basophilic
Eosin Y
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Objectives: This research was conducted to look for the vest conditions of Hematoxylin-eosin staining. Methods/ Statistical Analysis: Method was investigated the Hematoxylin-eosin staining, Microscopy and RGB analysis. Findings: The result was as follows. The thickness of the optimal condition is 3 cm, the time of the optimal Hematoxylin 4 minutes 30 seconds and eosin 3 minutes 45 seconds. Also, RGB analysis was green. So green showed a significant difference in the change width to each stage, and the biggest change was 52.74 at 2㎛ and 3㎛. Improvements: Therefore, an appropriate fixed time is needed to be found to get staining intensity and sharpness, and the freshness of reagent for each stage should be maintainedKeywords: Eosin, Hematoxylin, RGB, Microscopy, Stain
Eosin
Haematoxylin
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Eosin Y
RGB color model
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