The evaluation of ethyl eosin
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Eosin
Eosin Y
A staining schedule employing phloxine as a counter-stain to Erlich's acid hematoxylin is presented. Fixation is best with Zenker's fluid, although formalin can be used. The technic is similar to the standard hematoxylin-eosin formulae but because of the staining advantages of phloxine over eosin, the technic is simpler, and quicker, resulting in clearly differentiated sections which do not fade as soon as do eosin-stained slides. A brief summary of the uses of phloxine as a biological stain is given and its advantages over eosin are discussed.
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Eosin
Eosin Y
ALIZARIN RED
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Eosin
Eosin Y
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Objective: To establish a method of hematoxylin and eosin staining on the section after fluorescent labeling.Methods:Sections of paraffin and frozen ones stained with immunofluorescent or Hoechst dye were observed under a microscope.After washing away the mounting material glyencerol with 50% alcohol(pH 4.7-5.0),the slides were stained with HE.The effect of this procedure was analyzed.Results: The effect of such manipulation did not reduce the resolution.Information could be obtained both from light and fluorescence microscopes.Conclusion:We have introduced a new method to broaden the morphological information from one section without torturing the morphological structure.
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Reinke crystalloid (CR)s are rod or corn shaped structures present in the interstitial cells (Leydig cell) of human testis. Due to existing controversies and because of their importance in histological studies, we decided to reevaluate their staining behavior with various dyes. Earlier it has been shown that hematoxylin and eosin dyes do not stain CRs even though their protein nature remains undisputed. In the present study, sections of testicular tissues embedded in glycol-methacrylate (GMA) showed that hematoxylin binds to CRs non-specifically and eosin stains them specifically. The reasons for stainability and/or non stainability of CRs with hematoxylin, eosin and other dyes are discussed.
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Eosin Y
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Instead of two-stage staining with hematoxylin-eosin and hallocyanin-picrofuchsin a technique for simultaneous staining in mixtures of hallocyanin and eosin or picrofuchsin is suggested. The stains-cocktails are well preserved for 4-5 weeks and may be used repeatedly.
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Eosin Y
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No AccessJournal of Urology1 Dec 1977Supravital Staining of Spermatozoa: Relationship of Eosin Concentration to the Percentage of Cells Staining Live K.A. Dougherty, R.L. Urry, and A.T.K. Cockett K.A. DoughertyK.A. Dougherty More articles by this author , R.L. UrryR.L. Urry More articles by this author , and A.T.K. CockettA.T.K. Cockett More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)58278-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Supravital staining of human spermatozoa is a useful technique to assess semen quality. We compared 3 concentrations of eosin (1, 2.5 and 5 per cent) for their effectiveness to differentiate viable and non-viable spermatozoa. The percentage of viable cells determined by each concentration was compared as well as the percentage of cells estimated to be active. The results indicate that the percentage of spermatozoa determined to be viable with the supravital stains can be altered by changing the percentage of eosin in the stains. Use of 1 per cent eosin gave values that were significantly higher than the percentage of cells determined to be viable with 5 per cent eosin and the percentage of cells estimated to be active. Better quality slides were produced with 5 per cent eosin, which provided values that correlated favorably with motility estimations. © 1977 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 118Issue 6December 1977Page: 1008-1009 Advertisement Copyright & Permissions© 1977 by The American Urological Association Education and Research, Inc.MetricsAuthor Information K.A. Dougherty More articles by this author R.L. Urry More articles by this author A.T.K. Cockett More articles by this author Expand All Advertisement Loading ...
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Eosin Y
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배경: 조직표본제작 시 H&E(Hematoxylin and Eosin)염색은 조직의 형태학적 구조와 세포의 변화 정도를 판단할 수 있는 가장 일반적인 염색방법이다. 이 염색에 사용되는 Hematoxylin과 Eosin은 염료분자와 조직성분의 이온결합 기전에 의해 세포의 핵과 세포질에 각각 염색되며, 두 염료의 pH 변화에 따라 염색성이 달라질 수 있다. 따라서 본원에서 시행하고 있는 H&E 염색방법을 통해 pH 변화에 따른 조직표본의 염색성을 비교하고, 염료의 적정 pH와 pH 유지의 중요성을 알아보고자 한다. 방법: 본 실험은 Liver, Kidney, Lung 의 조직표본을 대상으로 실시하였다. Harris hematoxylin(영동제약, korea) 과 제조한 Eosin(Sigma Chemical, USA)을 사용하여 varistain XY(Shandon, USA)로 염색을 실시하였고 pH는 300 A digital pH meter(Bantex, USA)를 이용하여 측정하였다. pH에 따른 H&E염색의 염색성을 관찰하기 위하여 Harris hematoxylin과 Eosin 염료의 pH를 각각 8군(pH1.5~8.5)으로 나누어 염색을 실시하고, 광학현미경을 이용하여 염색성을 비교하였다. 결과: Harris hematoxylin의 경우 pH1.5, pH4.5에서 염색성이 저하되었고, pH2.5와 pH3.5에서 적정한 염색성을 보였으며, pH5.5~pH8.5에서 염색이 되지 않았다. Eosin의 경우 pH1.5, pH2.5, pH6.5에서 염색성이 저하되었고, pH3.5, pH4.5, pH5.5에서 적정한 염색성을 보였으며. pH7.5, pH8.5에서는 염색이 되지 않았다. 고찰: H&E염색은 pH의 변화에 따라 핵과 세포질의 염색성에 많은 차이를 보인다. 따라서 H&E염색의 염색성은 pH 변화에 영향을 받으므로 pH의 유지가 요구되며, 일정한 염색성을 가진 조직표본을 얻기 위해서는 검사실 실정에 맞는 지속적인 염료의 pH 관리가 필요하다고 사료된다.
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Eosin Y
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Mordant blue 3 may be used as a suhstitute for hematoxylin in hematoxylin and eosin stains. The staining solution consists of 0.25 g dye, 40 ml of 10% iron dam, 5 ml of cone H2SO4, and 955 ml of dirtilled H2O. Staining the is 5 minutes, followed by differentiation in acid water or acid alcohol. After blueing, the seaions are counterstained with emin. Results closely resemble the hematoxylin and eosin stain.
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Eosin
Eosin Y
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Eosin
Eosin Y
Bovine serum albumin
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A hematoxylin and eosin procedure is described for staining formalin-fixed tissues embedded in glycolmethacrylate with emphasis on 1.5 micron sections. The staining method utilizes Gill's hematoxylin followed by a buffered eosin Y-phloxine B counterstain. Acidophilic and basophilic components of most stained tissue sections had an intense, sharp and clean appearance when observed by light microscopy.
Eosin
ALIZARIN RED
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Basophilic
Eosin Y
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