G-banding in plant chromosomes
16
Citation
1
Reference
10
Related Paper
Citation Trend
Abstract:
G-banding was successfully demonstrated in chromosomes of cultured Vicia hajastana cells using a simple and reproducible technique. The factors considered important in achieving successful banding were (i) the omission of HCl hydrolysis or enzymatic digestion during the process of making the chromosome preparations; and (ii) the treatment with a weak NaHCO 3 solution at pH 9.0–9.5 prior to staining with Giemsa. The G-banding in prophase and prometaphase chromosomes was of better quality and more consistent than that in metaphase chromosomes.Key words: Vicia hajastana, cell line, G-banding.Keywords:
Prometaphase
G banding
Giemsa stain
Chromosome morphology
Orcein
Cite
Giemsa stain
G banding
Chromosome morphology
Cytotaxonomy
Cite
Citations (122)
This report describes staining techniques for chromosome banding and sister chromatid exchanges (SCEs) suited to a method that allows simultaneous analysis of cell morphology and karyotype. Mitotic cells are first identified by either cytochemical staining or immunologic methods. The preparations are then destained and treated with acid fixative. For G- and C-banding, the cells are incubated overnight at room temperature in Sørensen buffer and then stained with Giemsa. To demonstrate SCEs, the cells are fluorescent stained before being stained with Giemsa.
Giemsa stain
Fixative
G banding
Differential staining
Chromosome morphology
Cite
Citations (27)
Orcein
Giemsa stain
G banding
Constitutive heterochromatin
Chromosome number
Chromosome morphology
Cite
Citations (14)
Giemsa stain
Chromosome morphology
G banding
Differential staining
Cite
Citations (43)
Conventional staining techniques such as Giemsa, orcein and carmine and several differential staining techniques for the observation of mammalian chromosomes are described in this chapter. The differential staining techniques are described by classifying them into two groups; one group consists of banding stainings involving quinacrine (Q-band) and Giemsa (G-band), while the other involves several differential stainings for observation of the inner-organization of chromosomes, such as the constitutive and facultative heterochromatins, the nucleolar organizer regions and the centromeric regions.
Differential staining
Giemsa stain
Orcein
G banding
Cite
Citations (0)
The chromosomes from root tips of Lilium leucanthum(Baker)Baker were analyzed by Giemsa C-banding.The result showed that,the karyotype is 2n=2x=24,the banding number of one genome was 21.The C-banding karyotype would be:2n=24=6C+2CI+2I+2CI++2CI++4I++2I++2T++2I+S.Significantly different C-banding points were shown on each chromosome.Most of the strong bands of L.leucanthum were distributed on the centromeric regions of the chromosomes.Each chromosome of L.leucanthum(Baker)Baker can be distinguished by Giemsa C-banding.
Giemsa stain
G banding
Chromosome number
Chromosome morphology
Lilium
Cite
Citations (0)
G-banding was successfully demonstrated in chromosomes of cultured Vicia hajastana cells using a simple and reproducible technique. The factors considered important in achieving successful banding were (i) the omission of HCl hydrolysis or enzymatic digestion during the process of making the chromosome preparations; and (ii) the treatment with a weak NaHCO 3 solution at pH 9.0–9.5 prior to staining with Giemsa. The G-banding in prophase and prometaphase chromosomes was of better quality and more consistent than that in metaphase chromosomes.Key words: Vicia hajastana, cell line, G-banding.
Prometaphase
G banding
Giemsa stain
Chromosome morphology
Orcein
Cite
Citations (16)
Giemsa stain
G banding
Chromosome morphology
Cite
Citations (181)
The studies of the karyotype and Giemsa C banding pattern of cultivated barley suggest that the karyotype of cultivated barley is 2n=2x=14=12m(2SAT)+2sm(SAT), and that its Giemsa C band proves rich in bands. The studies not only further enrich the research on the Giemsa C banding and the cultivated barley, but also offer evidence and reference for the further genetic analysis for barley and its generation of crossbreed.
Giemsa stain
G banding
Chromosome morphology
Cite
Citations (0)
Giemsa stain
G banding
Chromosome morphology
Chromosome number
Cite
Citations (227)