Chromosome Giemsa C-banding Analysis of Lilium leucanthum(Baker) Baker
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The chromosomes from root tips of Lilium leucanthum(Baker)Baker were analyzed by Giemsa C-banding.The result showed that,the karyotype is 2n=2x=24,the banding number of one genome was 21.The C-banding karyotype would be:2n=24=6C+2CI+2I+2CI++2CI++4I++2I++2T++2I+S.Significantly different C-banding points were shown on each chromosome.Most of the strong bands of L.leucanthum were distributed on the centromeric regions of the chromosomes.Each chromosome of L.leucanthum(Baker)Baker can be distinguished by Giemsa C-banding.Keywords:
Giemsa stain
G banding
Chromosome number
Chromosome morphology
Lilium
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A Giemsa staining technique has been applied to the somatic chromosomes of the inbreeding diploid grass Lolium temulentum (2n = 14). Bands appear in six of the seven pairs and are associated either with the centromere or secondary constriction. An idiogram of the chromosomes with their banding patterns is presented.
Giemsa stain
Secondary constriction
Lolium
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Extensive polymorphism was found with regard to the presence and size of Giemsa-staining bands in the chromosomes of six inbred lines of cultivated rye (Secale cereale L.). The amount of polymorphism differed from chromosome to chromosome, with 6R being the most variable and 3R or 7R the least.
Giemsa stain
Secale
Chromosome morphology
G banding
Bivalent (engine)
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Lilium
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In this paper,the HKG(HCl-KOH-Giemsa)method studied and analysised the C-banding of chromosomes in triple cross of Inner Mongolia Hibrid oil sunflower 3.The results showed that every chromosome had at least one C-banding and there were 62 C-bandings on the chromosome pairs,most of which were centromeric and interbands,and the interbands were mainly distributed on the short arms;C-banding intensity were different,46 were strong and 16 were weak.The C-bandings formula was 2n=2x=34=8I++3T++5I+I+T++4C+2CI+4CI++3CI++I+T++CT++2CT+.Each chromosome had its own unique band pattern distinguishable from all the others,so each chromosome of sunflower can be distinguished by Giemsa C-banding.
Giemsa stain
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To study Aquilaria sinensis (Lour.) Spreng chromosome karyotype and C-banding.Sections for karvotvpe and BSG method for C-banding.The somatic chromosome number was 2n=16, karyotype formula was K(2n) = 2x = 16 = 6m + 6sm + 4st, based on Levan's publication in 1964. According to the method of Kuo, the chromosome based on the relative length was 2n = 16 = 4L + 4M2 + 6M1 + 2S, which belonged to "2B". 8 pairs chromosomes had 34 C-bands and the C-banding patter was 2n = 2x = 16 = 4C + 2I + 2T + 2CI+ + 2CI+ T + 2CI+ T+ + 2I+ T+.The data of karyotype and C-banding indicated A. sinensis chromosome had a relatively high asymmetry and was in the advanced stage of evolution, which offered the evidence for further genetic analysis.
Chromosome number
G banding
Giemsa stain
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Giemsa N-banding pattern in some tetraploid taxon of Hordeum murinum was studied. An ideogram was developed for each studied taxon of Hordeum murinum for the description of individual N-bands. N-banded karyotype of tetraploid taxa of H. murinum had 4-6 bands per chromosome, subsp. murinum showed 5 and subsp. leporinum 6 bands per chromosome on an average. Most of the bands were intercalary. The pattern showed high levels of band heteromorphism and banding pattern polymorphism but heteromorphisms were not observed between homologous chromosomes within these taxa.
Hordeum
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Twenty barley lines studied had a common basic chromosome banding pattern. Most bands ranged from medium to very small in size. The most conspicuous banding occurred at or near the centromeres, in the proximal, intercalary parts of most chromosome arms, and beside the secondary constrictions of the satellited chromosomes. Small to very small bands were always present at the chromosome ends. In addition, some lines had one distinct, intercalary band in the distal half of the long arm of one or more non-satellited chromosomes. Fifteen locations out of thirty-three with distinct bands showed band heteromorphy; the heteromorphy was the result of variation in band size or of the presence or apparent absence of a particular band. The band heteromorphy resulted in two different banding pattern variants of chromosomes 1 and 4, three of chromosomes 2 and 5, four of chromosome 3, six of chromosome 6, and eight of chromosome 7. Seventeen differently banded karyotypes were found. Some banding pattern polymorphisms can be used in cytological and cytogenetic studies.
G banding
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The karyotype of Lilium brownii F.E.Brown var.viridulum Baker was analyzed by conventional pressed disc method.The results indicated that the chromosome numbers were 2n=24 and the karyotype formulae was 2n=2x=24=4m(SAT) + 2sm + 10st(2SAT)+8t,the type of the karyotype belonged to 3B.As.K was 76.80%,the coefficient of chromosome relative length I.R.L = 6L+4M2+10M1+4S.
Lilium
Chromosome number
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Chromosome identification has been greatly improved by the analysis of differential chromosome fluorescence after fluorochrome treatment, and preferential Giemsa staining of certain chromosomal region resulting in specific Giemsa banding patterns after incubation in solutions of various reagent prior to the Giemsa staining.Lilium lancifolium has been known as triploid species.The conventional karyotype of this species was investigated (Kumazawa and Kimura 1947;Noda 1966).The basic chromosome complement (x=12) in this species consists of two metacentric and ten subtelocentric chromosomes.
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