Bilirubin Interference in the Diphenylamine Reaction
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Diphenylamine
Deoxyribose
Cytosine
Trichloroacetic acid
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Diphenylamine
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Diphenylamine
Deoxyribose
Cytosine
Trichloroacetic acid
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Cytosine
Thymine
Deoxyribose
Uracil
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Diphenylamine
Nitrobenzene
Colored
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In the presence of acid, the 2-deoxypentose of DNA is converted to beta-hydroxylevulinaldehyde which reacts with diphenylamine to give blue color, which is estimated by UV spectrophotometer or by colorimeter at 595 nm. Since only deoxyribose of purine nucleotide reacts, the value obtained represents only half to total amount.
Diphenylamine
Deoxyribose
Colorimeter
Bleach
Value (mathematics)
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Summary Dips of diphenylamine 1,000 p.p.m. in aqueous alcoholic solution controlled superficial scald on 11 varieties of apples, but not on Statesman. A similar formulation applied as a spray to fruit in cases was equally successful, but some damage occurred to the bottom fruit where drainage was incomplete. Paper wraps containing DPA completely controlled scald in Granny Smith apples. Oiled wraps did not give adequate control of scald in immature, early pieked fruit. Phenothiazine 1,000 and 2,000 p.p.m. did not control scald and caused damage to some varieties. Several other unsuccessful materials are noted. Diphenylamine does not aggravate other storage disorders, and there is some evidence that it may reduce both internal breakdown and core flush.
Diphenylamine
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The method under discussion is based on varying stability of a glycoside bond of purine and pyrimidine bases with deoxyribose. The DNA methylated with SAM (CH3-3H) and precipitated on GF/C filters was incubated in 0.5 N HCl for 40 min at 60 degrees. Under these conditions 6-methyladenine was released completely, while a significant amount of 5-methylcytosine was preserved.
Deoxyribose
Cytosine
5-Methylcytosine
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Diphenylamine
Deoxyribose
Absorbance
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Infrared spectroscopy was used to study films of the type I complex of Ag + and DNA as a function of hydration with the following conclusions. (1) Ag + binds to guanine residues but not to cytosine or thymine residues. (2) Cytosine becomes protonated as Ag + binds to guanine. (These conclusions confirm previous models.) (3) The type I complex remains in the B family of structures with slight modifications of the sugar-phosphate geometry. (4) This modified B structure remains stable at lower values of hydration for which pure DNA is in the A form. (5) Binding of Ag + to PO 2 O-P-O or the deoxyribose oxygen is excluded.
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To understand why 3′,5′-phosphodiester bond is ultimately chosen by the nature as the DNA structural framework,in this article,the energy of the phosphoric acid,2′-ribodesose,cytosine,as well as their compounds phosphoric acid-2′-ribodesose,2′-ribodesose-cytosine and phosphoric acid-2′-ribodesose-cytosine were calculated following thermodynamic method.It was found that the energy of 3′-deoxynucleotide,5′-deoxynucleotide,1′-deoxycytidine mono-phosphate,1′,3′-cytidine monophosphate,1′,5′-cytidine monophosphate is lower than that of the other resembling and non-resembling compounds,implying that there is a thermodynamical reason that the hydroxyl groups on the 2′-deoxyribose link to the phosphate groups to form DNA backbone.
Phosphodiester bond
Cytidine
Cytosine
Deoxyribose
Phosphoric acid
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