Estimation of DNA by Diphenylamine Reaction
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Abstract:
In the presence of acid, the 2-deoxypentose of DNA is converted to beta-hydroxylevulinaldehyde which reacts with diphenylamine to give blue color, which is estimated by UV spectrophotometer or by colorimeter at 595 nm. Since only deoxyribose of purine nucleotide reacts, the value obtained represents only half to total amount.Keywords:
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Deoxyribose
Colorimeter
Bleach
Value (mathematics)
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Abstract A separatory funnel procedure is presented that separates diphenylamine (DPA) from Ext. D&C Yellow No. 1. The DPA is extracted from the color with isooctane and the amount of DPA is determined spectrophotometrically. Recoveries of 0.02–1 % added DPA ranged from 95.5 to 104%.
Diphenylamine
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Current sitation at home and abroad,production methods and uses of diphenylamine were described.It was suggested that continuous condensation of aniline be used,plant for production of diphenylamine be constructed as soon as possible to meet the increasing need of diphenylamine.
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Summary Dips of diphenylamine 1,000 p.p.m. in aqueous alcoholic solution controlled superficial scald on 11 varieties of apples, but not on Statesman. A similar formulation applied as a spray to fruit in cases was equally successful, but some damage occurred to the bottom fruit where drainage was incomplete. Paper wraps containing DPA completely controlled scald in Granny Smith apples. Oiled wraps did not give adequate control of scald in immature, early pieked fruit. Phenothiazine 1,000 and 2,000 p.p.m. did not control scald and caused damage to some varieties. Several other unsuccessful materials are noted. Diphenylamine does not aggravate other storage disorders, and there is some evidence that it may reduce both internal breakdown and core flush.
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