High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays

Veronique Lecault,Michael VanInsberghe,Sanja Sekulovic,David J.H.F. Knapp,Stefan Wöhrer,William Bowden,Francis Viel,Thomas McLaughlin,Asefeh Jarandehei,Michelle Miller,Didier Falconnet,Adam K. White,David G. Kent,Michael R. Copley,Fariborz Taghipour,Connie J. Eaves,R. Keith Humphries,James M. Piret,Carl L. Hansen

High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays

2011

Veronique Lecault
Michael VanInsberghe
Sanja Sekulovic
David J.H.F. Knapp
Stefan Wöhrer
William Bowden
Francis Viel
Thomas McLaughlin
Asefeh Jarandehei
Michelle Miller
Didier Falconnet
Adam K. White
David G. Kent
Michael R. Copley
Fariborz Taghipour
Connie J. Eaves
R. Keith Humphries
James M. Piret
Carl L. Hansen

A microfluidic setup for nanoliter-volume perfused clonal culture and imaging of thousands of nonadherent cells is applied to study signaling and proliferation in hematopoietic stem cells.

Keywords:

Live cell imaging
Hematopoietic stem cell proliferation
Stem cell
Cell growth
Stem cell factor
Molecular biology
Cell biology
Haematopoiesis
Cell culture
Biology
Hematopoietic stem cell
Microfluidics
Lab-on-a-chip
High throughput analysis

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