Determination of polarizability and surface concentration of biomolecules using surface plasmon resonance experiment

Both surface molecules concentration Ns and polarizability Am can be determined from the surface plasmon experiment instead of effective layer thickness, which is usually determined. The new method of quantitative comparison of experimental and calculated surface plasmon resonance (SPR) curves is proposed. Mathematical minimization of curves difference is used in this case. It is shown, that it is possible to determine both Am and Nm from one measurement for accurate experiment and high quality optical system.