Perivascular deletion of murine Rac reverses the ratio of marrow arterioles and sinusoid vessels and alters hematopoiesis in vivo

Hematopoietic stem cells (HSCs) are localized within specialized microenvironments throughout the bone marrow. Nestin-expressing (Nestin + ) mesenchymal stromal cells (MSCs) are important in the perivascular space. Rac is critical for MSC cell shape in vitro, while its function in MSCs in vivo remains poorly characterized. We hypothesized that deletion of Rac in the Nestin + cells would perturb the perivascular space altering HSC localization and hematopoiesis. Nestin-Cre-directed excision of Rac1 in Rac3 -/- mice reduces Nestin + cells in the marrow. We observed 2.7-fold decrease in homing of labeled WT hematopoietic cells into Rac1 Δ/Δ Rac3 -/- mice compared with control mice. Rac1 Δ/Δ Rac3 -/- mice demonstrated a marked decrease in arterioles and increase in the number and volume of venous sinusoids in the marrow that was associated with a reduction in the numbers of immunophenotypically and functionally defined long-term HSCs in the marrow, a decrease in colony forming cells and a reduction in circulating progenitors. Rac-deleted animals demonstrated a significant increase in trabecular bone. These data demonstrate that Rac GTPases play an important role in the integrity of perivascular space. Increased trabecular bone and sinusoidal space and decreased in arteriolar volume in this model was associated with decreased HSC, underscoring the complexity of regulation of hematopoiesis in the perivascular space.