Screening of the candidate proteins interacting with TaFRA

F-box protein is an important subunit of SCF complex,an E3 ligase in ubiquitin system,and its function is determined through mediating the specific recognition and combining with substrate protein.TaFRA(F-box protein related to abiotic stress) was identified by RACE based on the fragments diferently expressing in wheat seedling exposed to salt stress and encodes an F-box protein.In this study,pBD-TaFRA bait expression vector was constructed,and cDNA+pGAD+pBD was directly co-transformed into yeast hybrid system to screen condidate proteins interacting with TaFRA.Fourty-four candidate proteins were obtained,in which 32 were known proteins and transcript factors related to stress tolerance such as thioredoxin,metallothinein,ATP synthase,and serine/threonine protein kinase etc.This indicates that TaFRA participates in stress response through regulating above condidate genes,which will provide basis for revealing the mechanism of TaFRA reaction to abiotic stress.