Abstract An antitumour‐promoting activity in two‐stage carcinogenesis, is found in the methanol extract of the Carthami Flos ( Carthamus tinctorius L.; Compositae), which is a traditional Chinese medicine and natural pigment of rouge additivies in certain Asian countries. From these active fractions, δ 5 ‐ and δ 7 ‐sterol fractions were separated. The separation was examined for inhibitory activity against TPA‐induced inflammatory ear oedema in mice. Stigmasterol (71% in the mixture) was the most abundant of 14 sterols identified in the δ 5 ‐sterol fraction. Schottenol (70% in the mixture) constituted the dominant sterol of the δ 7 ‐sterol fraction. Furthermore, stigmasterol markedly inhibited tumour promotion in two‐stage carcinogenesis experiments.
Journal Article Virus-specific Proteins Produced in HeLa Cells Infected with Poliovirus: Characterization of Subunit-like Protein Get access YASUSHI WATANABE, YASUSHI WATANABE Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar KIKUKO WATANABE, KIKUKO WATANABE Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar SUSUMU KATAGIRI, SUSUMU KATAGIRI Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar YORIO HINUMA YORIO HINUMA Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 57, Issue 6, June 1965, Pages 733–741, https://doi.org/10.1093/oxfordjournals.jbchem.a128139 Published: 01 June 1965 Article history Received: 18 December 1964 Published: 01 June 1965
This investigation was carried out to clarify the cause of food poisoning following the consumption of wild grasses in Yamagata prefecture. The food poisoning was suspected to have been caused by Aconitum roots mixed with edible roots, because the toxic symptoms were similar to those induced by Aconitum plants.Several aconitine analogues (aconitine, hypaconitine, mesaconitine) present in the food poisoning sample were resolved by high performance liquid chromatography (HPLC) using an ODS column and by liquid chromatograph-mass spectrometry (LC/MS). The contents of mesaconitine was 0.415μg/g, and that of aconitine was 0.153μg/g in the food poisoning sample.The toxic symptoms in mice given the food poisoning sample (150g/kg, i. p.) were convulsion, vomitting, salivation, diarrhea and death. Mesaconitine (0.1mg/kg, i. p.) showed similar toxic symptoms.These results led to the conclusion that the food poisoning has been caused by mesaconitine and aconitine from Aconitum roots mixed with the edible plants. Quantitative analysis of wild Aconitum roots collected in Yamagata city confirmed that mesaconitine was the main principle, rather than aconitine or other alkaloids.
Flagellar antigen of Bacillus cereus H.1 was purified and tested for serodiagnostic antigen by ELISA. The antibody against the flagellar antigen of B. cereus H.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of B. cereus. This common flagellar antigen of B. cereus was found to be due to 61-kDa protein by SDS-PAGE and immunoblot assay. Monoclonal antibody H15A5 against common antigenic epitope of B. cereus also reacted with flagellar antigens of 21 strains of Bacillus thuringiensis by ELISA. This monoclonal antibody reacted with the 61-kDa protein of the flagella of B. cereus H.1 and H.2 and B. thuringiensis Kurstaki HD1, Alesti and Aizawai juroi by immunoblot analysis. These results indicated that the common antigenic epitope of the 61-kDa protein existed in the flagella both of B. cereus and B. thuringiensis.
Highly purified poliovirus possessing N reactivity (N antigen) and H antigen which was prepared by heating the former, were used to pursue the production of complement-fixing antibodies in guinea pigs. By four successive intraperitoneal injections of approximately 1.0μg of viral protein of the N antigenicity, both N and H antibodies were produced in all guinea pigs tested. By the same procedure and the same amount of viral protein of the H antigenicity, the animals produced only H antibody. However, titers of H antibody were greatly varied from animal to animal in contrast with those observed in the animals injected with N antigen. Practical purpose to obtain mono-reactive N or H antisera was accomplished by using absorption technique. and
