Virus-specific Proteins Produced in HeLa Cells Infected with Poliovirus: Characterization of Subunit-like Protein*
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Journal Article Virus-specific Proteins Produced in HeLa Cells Infected with Poliovirus: Characterization of Subunit-like Protein Get access YASUSHI WATANABE, YASUSHI WATANABE Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar KIKUKO WATANABE, KIKUKO WATANABE Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar SUSUMU KATAGIRI, SUSUMU KATAGIRI Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar YORIO HINUMA YORIO HINUMA Department of Bacteriology, Tohoku University, school of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 57, Issue 6, June 1965, Pages 733–741, https://doi.org/10.1093/oxfordjournals.jbchem.a128139 Published: 01 June 1965 Article history Received: 18 December 1964 Published: 01 June 1965Keywords:
Bacteriology
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Objective Sodium glycididazole(CMNa) was a novel tumor radiosensitizer,used on radiosensitizing research of laryngeal carcinoma, pharyngeal cancer, and lung cancer. It had obtained remarkable effection. The effects of CMNa on chemotherapy was still not affirmed. This research took the cervical adenocarcinoma HeLa cells as target cells, analyzed whether CMNa effected the chemosensitivity of cisplatin on cervical adenocarcinoma HeLa cells. Methods HeLa cells were subjected to cisplatin(DDP), CMNa, and both of them in various concentrations for 24 hours, and the morphology of the HeLa cells was observed. MTT colorimetry was used to determine the effects of the drugs on HeLa cells. Results The HeLa cells treated with different concentrations of cisplatin underwent cell proliferation inhibition. Various concentrations of CMNa were not effected the HeLa cells. CMNa could strengthen the effects of low-concentrated DDP on HeLa cells greatly. Conclusion The combination of CMNa and DDP could not only enhance the sensitivity of DDP to HeLa cells, but also decrease the dosage of DDP,which could provide theoretical guidance for cervical carcinoma.
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Objective To investigate the effect of SAHA(suberoylanilide hydroxamic acid)on p53 wild type HeLa cells and p53 knock-down HeLa-E3 cells.Methods The cell's proliferation were determined by MTT after 24,48 and 72 h incubation;the cell cycle distribution and cell death were detected by flow cytometry and typan-blue assay,respectively.Results SAHA significantly suppressed the in vitro proliferation of HeLa and HeLa-E3 cells in a time and dose dependent manner.G2/M arrest and cell death of HeLa cells were also elevated by SAHA in 24 h,while HeLa cells were more sensitive to SAHA than HeLa-E3 cells.Conclusion The results show that the sensitivity of tumor cells to SAHA were influenced by p53.
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Objective To investigate the apoptosis inducing effect of N-4-hydroxyphenyl retinode(4-HPR)and Cisplatin on human HeLa cell lines.Methods MTT assay was employed to examine the growth suppression of the cells.TEM was used to observe the ultrastructural change of HeLa cells treated with 4-HPR and Cisplatin.The apoptosis rate and change in cell cycle of HeLa cells treated with 4-HPR or/and Cisplatin were examined by flow cytometer.Results 4-HPR and Cisplatin both could induce apoptosis of HeLa cells;combined use of 4-HPR and Cisplatin could enhance HeLa cells apoptosis.Conclusion 4-HPR and Cisplatin could induce apoptosis of HeLa cells,and their effect is synergetic.
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Objective:To observe whether the serum pharmacological effect of FL inhibits Hela cell proliferation in cervical cancer.Methods:We used MTT assay to examine the serum pharmacological effect of FL on the decrease im Hela cell,we used TUNEL for quantitive determination of the apoptosis of Hela cell.Results:The FL serum accelerated Hela cell apoptosis..Conclusion:The serum pharmacological effect of FL controls proliferation of Hela cell.which is very important for the gaidance in clinical treatment by the drug.
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Objective To observe the inhibition effect of the extract B of Polygonatum odoratum(EB-PAOA) on human cervix cancer Hela cells.Methods Cervix cancer Hela cells were cultured in vitro and then different concentration of EB-PAOA were added to Hela cells.The growing curve was drawn.MTT assay was adopted to determine the inhibition rate to Hela cells.Results EB-PAOA can inhibit the proliferation of Hela cells,and the inhibition rate is of time and dose dependent.Conclusions EB-PAOA has obvious inhibition effect on Hela cells.
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Objective To explore the effects of iNOS inhibitor 1400 W alone or combined with cisplatin to cervical cancer cell line HeLa. Methods Cervical cancer cell line HeLa was resuscitated and incubated,MTT was used to investigate 1400 W and cisplatin’s effects on HeLa cell growth; flow cytometry was used to detect the HeLa cell apoptosis rate and RT-PCR was used to detecte the E6, p53 mRNA expression of HeLa cell. Results Both cisplatin and 1400 W showed a dose-dependent inhibitory effect on HeLa cell proliferation(P0.05). 1400 W could not increase HeLa cell’s apoptosis, while 1400 W combined cisplatin could both induce apoptosis and decrease E6, p53 mRNA expression of HeLa cell(P0.05). Conclusions 1400 W combined with ciplatin can increase the HeLa cell apoptosis rate than the group cisplatin used alone.Combined with iNOS inhibitor, it can enhance the cisplatin effect on tumor cell. This is possible a promising adjuvant therapy for cervical caner.
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フッ素による培養細胞の増殖抑制機構を検討するため, HeLa細胞ならびにフッ素耐性HeLa細胞 (FR細胞) を用いて実験を行った。耐性細胞は最高2mMのNaFを添加した培地でHeLa細胞を継代し樹立したものである。その結果次の成績が得られた。1. HeLa細胞の増殖は, 0.5mMNaFでわずかに抑制され, 2mMNaFでは全く阻止された。FR細胞は, 2mM NaFでも対照と同じ増殖を示した。2. 2mMNaF存在下でHeLa細胞のタンパク質合成は強く阻害されたが, FR細胞でのタンパク質合成阻害はわずかであった。3. HeLa細胞とFR細胞の細胞質のタンパク質組成をSDS-ポリアクリルアミドゲル電気泳動 (SDS-PAGE) で調べたところ, クーマシーブルー染色で, 両細胞間のペプチドバンドのパターンに明らかな違いは認められなかった。4. HeLa細胞とFR細胞を, 2mM NaF処理した後合成されたタンパク質をSDS-PAGE, オートラジオグラフィーで調べたところ, HeLa細胞では, 分子量約77000ダルトンの強くラベルされる特異的なペプチドが認められた。FR細胞では, NaFの作用をうけないHeLa細胞と同様なペプチドバンドのパターンが見られた。5. この特異的な77000ダルトンのペプチドは, フッ素処理時間の増加と共に強くラベルされる傾向を示した。6. 77000ダルトンのペプチドの細胞内局在は, 形質膜, ミトコンドリア分画, ミクロゾーム分画に認められたが, 核, 可溶性分画には認められなかった。
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Objective To observe the effects of different concentration of TRAIL on cervical cancer HeLa cells.Methods With different concentration of TRAIL role in HeLa cells,the survival fraction of HeLa cells was measured by MTT assay in Chongqing Medical University in 2006.Structures of apoptosis cells were observed by AO/EB fluorescence microscopy.Cell cycle of HeLa cells and apoptosis rates were determined by FACS.Results After treating HeLa cells for 24 h by TRAIL,a good dose-effect relationship was demonstrated by the role of TRAIL in the rate of cell growth inhibition and apoptosis;early and late apoptosis cell can be seen by AO/EB stainning and the hypodiploid peaks were found by FACS checking.The cell cycles were changed.Conclusion The proliferation of HeLa cells can be inhibited and the apoptosis can be induced by TRAIL.
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Objective To investigate the inhibiting effect of artemisinin(ART)on HeLa cell growth in vitro. Methods The antiproliferation activities of ART on HeLa cells were evaluated by MTT assay.The morphological changes of ART on HeLa cells were observed under transmission electronmicroscopy.Cell cycle and apoptosis of HeLa cells influenced by ART were assessed by a flow cytometer.The telomerase activity was detected by immunohistochemistry.Results After HeLa cells were treated by ART for 72 h,the cell growth was inhibited in vitro.The inhibiting effect was increased gradually following the increase of ART concentration.The percentage of Hela cells in S phase was decreased,but increased in G_1 phase after treatment with ART.After 72 h treatment,apoptosis was induced on HeLa cells,and hTRT gene of HeLa cell was decreased obviously. Conclusion ART can inhibit the growth of human HeLa cells,induce the apoptosis,and reduce the telomerase activity.
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Objective To study the effect of 19 kinds of taxnae and related analogues from T.cuspidata on the proliferation of HeLa cervical cancer cells.Methods The effect of 19 kinds of taxnae and related analogues from T.cuspidata on the proliferation of HeLa cervical cancer cells were evaluated by MTT assay.Results After treatment with taxnae and related analogues(10~(-4) mol/L) for 48h,Compound 5,7,and 16 inhibited the proliferation of HeLa cells significantly(P0.01 ).The survival rate of HeLa cells was 26.93%,20.79%and 32.82%,respectively.In the 10~(-6)～10~(-4) mol/L concentration range,the Compound 3 and 14 showed a definite dose-dependent relationship.Conclusion The five types of taxanes can inhibit the proliferation of cervical cancer HeLa cells to different extents.
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