Xingyuan Cao

China Agricultural University

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Jianzhong Shen

China Agricultural University

Suxia Zhang

Xi'an University of Technology

Jianzhong Wang

China Agricultural University

Zhanhui Wang

Chinese Academy of Sciences

Jicheng Qiu

China Agricultural University

Jingyuan Kong

China Agricultural University

Feifei Sun

Kunming Institute of Botany

Pan Sun

China Agricultural University

Suxia Zhang

Tianjin University

Shupeng Yang

Institute of Food Science and Technology

Cooperative Institutions

China Agricultural University

108

Ministry of Agriculture and Rural Affairs

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Beijing VDJBio (China)

China Institute of Veterinary Drug Control

Ghent University

Chinese Academy of Medical Sciences & Peking Union Medical College

Anhui Agricultural University

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Development of a chemiluminescent competitive indirect ELISA method procedure for the determination of gentamicin in milk

Analytical Methods (2012)

Yanshen Li Yanrong Zhang Xingyuan Cao Zhanhui Wang Jianzhong Shen

A rapid and sensitive method based on chemiluminescent competitive indirect ELISA (CL-ciELISA) was developed for the screening of gentamicin (GEN) in milk. The cross-reactivity (CR) of gentamicin, sisomicin (SIS), neomycin (NEO), kanamycin (KAN), neamine (NEA), streptomycin (STR), apramycin (APR) and spectomycin (SPT) with this GEN-specific monoclonal antibody (MAb) produced in this experiment were 100, 3.2, <0.1, <0.1, <0.1, <0.1, <0.1, and <0.1%, respectively. In this method, IC50 (half maximal inhibitory concentration of a substance) of the MAb was 0.69 ng mL−1 and the limit of detection (LOD), measured by IC10, was 0.06 ng mL−1. This CL-ciELISA method with luminol solution as a substrate was 10 times more sensitive compared to the colorimetric-ciELISA. In spiked milk samples, the mean recoveries ranged from 85.6–97.9% with intra-day and inter-day variation less than 7.9 and 7.98%, respectively. The results illustrated that this procedure is applicable for screening gentamicin in milk.

Sisomicin

Kanamycin

Coefficient of variation

Neomycin

10.1039/c2ay25141h

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Citations (13)

A one-step chemiluminescence immunoassay for 20 fluoroquinolone residues in fish and shrimp based on a single chain Fv–alkaline phosphatase fusion protein

Analytical Methods (2015)

Xuezhi Yu Xiaoqi Tao Jianzhong Shen Suxia Zhang Xingyuan Cao

A simple and rapid chemiluminescence competitive direct enzyme-linked generic immunosorbent assay was developed for 20 FQs in fish and shrimp samples.

10.1039/c5ay01410g

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Citations (19)

Ultra-performance liquid chromatography–tandem mass spectrometry determination and depletion profile of flunixin residues in tissues after single oral administration in rabbits

Journal of Chromatography B (2013)

Ailing Zhu Tao Peng Liang Liu Xi Xia Ting Hu

10.1016/j.jchromb.2013.07.002

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The pharmacokinetics of buserelin after intramuscular administration in pigs and cows

BMC Veterinary Research (2022)

Jingyuan Kong Fuqin Su Yu Liu Yuxin Yang Yuying Cao

Abstract Background Buserelin is a luteinizing hormone releasing hormone (LHRH) agonist used for the treatment of hormone-dependent diseases in males and females. However, the pharmacokinetics of buserelin in pigs and cows are not fully understood. This study was designed to develop a sensitive method to determine the concentration of buserelin in blood plasma and to investigate the pharmacokinetic parameters after intramuscular (i.m.) administration in pigs and cows. Results A sensitive and rapid stability method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed. The pharmacokinetic parameters of buserelin after i.m. administration were studied in five pigs and five cows at a single dose of 1 mg per pig and 3 mg per cow. The plasma kinetics were analyzed by WinNonlin 8.1.0 software using a non-compartmental model. The mean concentration area under the curve (AUC 0-t ) was 25.02 ± 6.93 h × ng/mL for pigs and 5.63 ± 1.86 h × ng/mL for cows. The maximum plasma concentration (C max ) and time to reach the maximum concentration (t max ) were 10.99 ± 2.04 ng/mL and 0.57 ± 0.18 h for pigs and 2.68 ± 0.36 ng/mL and 1.05 ± 0.27 h for cows, respectively. The apparent volume of distribution (V z ) in pigs and cows was 80.49 ± 43.88 L and 839.88 ± 174.77 L, respectively. The elimination half-time (t 1/2 ), and clearance (CL) were 1.29 ± 0.40 h and 41.15 ± 11.18 L/h for pigs and 1.13 ± 0.3 h and 545.04 ± 166.40 L/h for cows, respectively. No adverse effects were observed in any of the animals. Conclusion This study extends previous studies describing the pharmacokinetics of buserelin following i.m. administration in pigs and cows. Further studies investigating other factors were needed to establish therapeutic protocol in pigs and cows and to extrapolate these parameters to others economic animals.

Intramuscular injection

10.1186/s12917-022-03237-0

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Citations (5)

Purification of Nine Sulfonamides from Chicken Tissues by Immunoaffinity Chromatography Using Two Monoclonal Antibodies

Journal of AOAC International (2008)

Bingyu Li Cun Li Haiyang Jiang Zhahnui Wang Xingyuan Cao

Abstract An immunoaffinity chromatographic method was developed for the purification of 9 sulfonamides (sulfamethazine, sulfadimethoxine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamonomethoxine, and sulfapyridine) from chicken tissue (muscle and liver) samples. Two monoclonal antibodies (antisulfamethazine and antisulfamethoxazole) were simultaneously covalently coupled to CNBr-activated Sepharose 4B for the preparation of a re-usable immunoaffinity column. After extraction with methanolwater and purification by immunoaffinity column, the sulfonamides were determined by reversed-phase liquid chromatography and UV detection at 270 nm. The recoveries for each drug at fortification levels of 1050 ng/g ranged from 74.1 to 108.9 with relative standard deviations from 1.9 to 11.5. The limits of detection were 2 ng/g for each drug.

Sulfadimethoxine

Sulfamerazine

Sulfapyridine

Ractopamine

Sepharose

10.1093/jaoac/91.6.1488

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Citations (7)

Development of a UPLC-MS/MS method for quantifying KPT-335 (Verdinexor) in feline plasma for a study of PK

Frontiers in Veterinary Science (2024)

Yuxin Yang Jicheng Qiu Jingyuan Kong Yuying Cao Yu Liu

KPT-335 (Verdinexor) is a novel SINE that potently inhibits the nucleoprotein Exportin 1 (XPO1/CRM1) of tumor cell lines and reduces the replication level of the influenza virus. KPT-335 is mainly used for the treatment of canine tumors. Drugs for the effective treatment of feline tumors are currently unavailable in China. KPT-335 may have potential in the treatment of cat tumors. However, the effects of KPT-335 in cats are unreported, and no relevant methodology has been established for pharmacokinetic studies. In this study, a UPLC-MS/MS method was developed to determine KPT-335 concentrations in cat plasma, followed by pharmacokinetic studies. Briefly, plasma proteins are precipitated with acetonitrile, and the supernatant was collected for detection after centrifugation. The linearity for KPT-335 in cat plasma was in the range of 5-1,000 ng/mL. Satisfactory accuracy and precision were obtained. The intra-day accuracy was between -4.10% and 10.48%, the precision was ≤4.65%; the inter-day accuracy was between -0.11% and 8.09%, and the precision was ≤5.85%. Intra-day and inter-day accuracy and precision were within regulatory limits. The results of preliminary pharmacokinetic studies were as follows: T

10.3389/fvets.2024.1438295

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Pharmacokinetics of Tylvalosin Following Intravenous or Oral Administration at Different Doses in Broiler Chickens

Veterinary Sciences (2025)

Zeyu Wen Sumeng Chen Jinyan Meng Qinyao Wu Ruili Yu

Tylvalosin is a macrolide antimicrobial with antibacterial activity against Gram-positive bacteria, some Gram-negative organisms, and mycoplasma. It is used to treat respiratory and enteric bacterial infections in swine and poultry. In this study, we aimed to investigate the pharmacokinetic changes in tylvalosin following its intravenous or oral administration at doses of 5, 10, and 25 mg/kg in broiler chickens. Forty-eight broiler chickens were included in the study. The plasma concentrations of tylvalosin were measured by using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS), and its pharmacokinetic parameters were evaluated by using both non-compartmental and compartmental analyses. The linear mixed-effects model revealed no dose proportionality within the 5–25 mg/kg range for either administration route. Based on pharmacokinetic data from a single oral dose, this study simulated a multiple-dose regimen of tylvalosin (25 mg/kg), demonstrating that a 6-hour dosing interval reaches a steady state after the fourth dose. Furthermore, the results show that the absolute bioavailability of tylvalosin after oral administration in chickens was relatively low, with values of 5.92%, 3.56%, and 3.04% for the doses of 5, 10, and 25 mg/kg, respectively. Further studies are required to significantly improve the oral bioavailability of tylvalosin and similar compounds through formulation optimization.

10.3390/vetsci12020118

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Plasma Protein Binding Rate and Pharmacokinetics of Lekethromycin in Rats

Antibiotics (2022)

Pan Sun Hongzhi Xiao Jicheng Qiu Yuying Cao Jingyuan Kong

Lekethromycin (LKMS), a novel macrolide lactone, is still unclear regarding its absorption. Thus, we conducted this study to investigate the characteristics of LKMS in rats. We chose the ultrafiltration method to measure the plasma protein binding rate of LKMS. As a result, LKMS was characterized by quick absorption, delayed elimination, and extensive distribution in rats following intramuscular (im) and subcutaneous (sc) administration. Moreover, LKMS has a high protein binding rate (78-91%) in rats at a concentration range of 10-800 ng/mL. LKMS bioavailability was found to be approximately 84-139% and 52-77% after im and sc administration, respectively; however, LKMS was found to have extremely poor bioavailability after oral administration (po) in rats. The pharmacokinetic parameters cannot be considered linearly correlated with the administered dose. Additionally, LKMS and its corresponding metabolites were shown to be metabolically stable in the liver microsomes of rats, dogs, pigs, and humans. Notably, only one phase I metabolite was identified during in vitro study, suggesting most of drug was not converted. Collectively, LKMS had quick absorption but poor absorption after oral administration, extensive tissue distribution, metabolic stability, and slow elimination in rats.

Ultrafiltration (renal)

10.3390/antibiotics11091241

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Citations (3)

Determination of QLNC-3A6 in canine plasma by UHPLC-MS/MS and its application in pharmacokinetic studies

Veterinary Quarterly (2024)

Sumeng Chen Yu Liu Yue Wang Zeyu Wen Jinyan Meng

Multi-targeted tyrosine kinase inhibitor QLNC-3A6 Di-maleate, a structurally novel small molecule compound, has therapeutic efficacy for the treatment of canine cutaneous mast cell tumor (CMCT) caused by mutations in the c-Kit gene. Since pharmacokinetic (PK) information plays an important role in the development and application of new drugs, etc., a rapid, highly sensitive and selective UHPLC-MS/MS analytical method was developed and validated for the first time in this study for the quantitative detection of QLNC-3A6 in canine plasma. 100 µL of plasma was precipitated using 350 µL of acetonitrile, and Chromatographic separation was performed on a Phenomenex Kinetex C18 column (50 × 2.1 mm, 2.6 µm) at a flow rate of 0.4 mL/min, the mobile phases were set to 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile (B). The calibration curve linear range was 0.5-100 ng/mL (

10.1080/01652176.2024.2407174

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Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum

Energy & Fuels (2020)

Zhenyu Wang Xingyuan Cao Nan Li Zhengjiao Yang Ming Lei

Butanol is an attractive second-generation biofuel as well as an important chemical. Production of butanol from lignocellulosic feedstock via consolidated bioprocessing has attracted increasing interest in recent years. However, this has been limited by the inefficient degradation of extracellular polysaccharides in butanol-producing strains. In this study, production of butanol directly from hemicellulose was achieved simply through overexpression of an indigenous xylanase in Clostridium acetobutylicum. The xylanase (XynB) encoded by CA_P0053 was highly soluble and fully secreted from C. acetobutylicum. The extracellular xylanase activity was increased 88-fold, and 4.03 g/L biobutanol was obtained from hemicellulose, which has been the highest yield yet reported from either hemicellulose or xylan. This study proved that the solventogenic C. acetobutylicum had great potential for consolidated production of biofuels. Understanding and applying its secretion mechanism will be of great significance in the future.

Clostridium acetobutylicum

Hemicellulose

Lignocellulosic Biomass

10.1021/acs.energyfuels.9b04489

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Citations (10)