A one-step chemiluminescence immunoassay for 20 fluoroquinolone residues in fish and shrimp based on a single chain Fv–alkaline phosphatase fusion protein
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Abstract:
A simple and rapid chemiluminescence competitive direct enzyme-linked generic immunosorbent assay was developed for 20 FQs in fish and shrimp samples.A development about the immobilization of chemilumine scence sensor sensitive-substance is summarized in this artical.All kinds of chemiluminescence sensor such as chemiluminescence chemical sensor、electrical-chemiluminescence sensor、chemiluminescence immune sensor,chemiluminescence biosensor,chemiluminescence nucleic acid sensor,chemiluminescence germ sensor are incoluded.An expection about chemiluminescence sensor is given at the end of the paper and 37 literatures from home and abroad are cited.
Chemical sensor
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Chemiluminescence is a phenomenon in which an electronically excited fluorescent molecule is generated during the chemical reaction and emits light as a visual output. Although there have been numerous investigations of chemiluminescence so far, a lot of problems unsettled are remaining, for example, a question of what is the high-energy intermediates that interact with fluorophores. In this review, our investigations on chemiluminescence from the viewpoint of organic chemistry are described, emphasizing the mechanistic studies and the application of peroxyoxalate chemiluminescence. Employing various reactive oxalate derivatives, the kinetic studies allowed us to suggest the structures of the high-energy intermediates in the peroxyoxalate chemiluminescence. The chemiluminescence of the suitable phosphonate carbanions during the oxy-Wittig type reaction was also mentioned, in which the phospha-1,2-dioxetane is the most likely high-energy intermediate. Some chemiluminescence reactions were connected with ligand-metal ion host-guest chemistry to get a useful hint for further application of chemiluminescence to chemical detection.
Peroxyoxalate
Dioxetane
Phosphonate
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Abstract Sodium hypochlorite (NaOCl) induced decomposition of organic hydroperoxides gave strong chemiluminescence. Chemiluminescence intensity reached its maximum a few seconds after the addition of sodium hypochlorite and decreased to the background level in three min. Good linear relationships were observed between total chemiluminescence counts in three min and the amounts of hydroperoxides. This chemiluminescence method can be applied to the detection of low levels of lipid hydroperoxides.
Sodium hypochlorite
Hypochlorite
Luminol
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Abstract The aim of this study was to assess the effect of gamma radiation alone or in combination with heat on the immunoreactivity of shrimp. Peeled shrimp muscle was subjected to gamma radiation (1, 5, 10 and 15 kGy) followed by boiling (5 min, 10 min and 15 min). Shrimp protein extract (PE) and a major allergen (Pen a 1) from treated shrimp were analysed by SDS‐PAGE and immunoblot using a specific IgE antibody from patients who were allergic to shrimp. Results showed that gamma radiation + heat could significantly affect shrimp immunoreactivity. The immunoreactivity of PE and Pen a 1 was also determined by using competitive inhibition ELISA (Ci‐ELISA). The IC 50 of PE and Pen a 1 from shrimp treated with gamma radiation + heat was 5‐ to 30‐fold higher than that of untreated shrimp. The immunoreactivity of gamma irradiated shrimp decreased with increasing dose of radiation. Copyright © 2007 Society of Chemical Industry
Penaeus
Penaeus monodon
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In this paper,the recent developments in chemiluminescence sensors were reviewed.Most of the literature reviewed dates from 1999.The content included design of chemiluminescence sensor,enzyme-based chemiluminescence biosensor,tissue-based chemiluminescence sensor,chemiluminescent immunosensor,ctaluminescence gas sensor,molecular imprinting-based chemiluminescence sensor and chemiluminescence sensor with chemiluminescence reaction as recognition reaction.The future of the chemiluminescence sensor was also forecasted.
Luminescent Measurements
Luciferases
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Vannamei shrimp (Litopenaeus vannamei) holds significant economic importance in Shrimponesia as a prominent shrimp commodity. The post-larval stage often encounters challenges, primarily due to the shrimp's limited ability to adapt and survive in a new environment. Stocking density is a crucial factor influencing the growth and production performance of vannamei shrimp culture. This study aims to assess the optimal stocking density for achieving optimal growth and survival in L. vannamei. A 40-day maintenance trial was conducted at UD Mina Rahayu Kalianda to investigate the impact of different stocking densities on water quality in the maintenance media. The study utilized containers with varying stocking densities: treatment A (1 shrimp/L), treatment B (1.5 shrimp/L), and treatment C (2 shrimp/L), each with three replications. The findings revealed survival rates (SR) of 83.33% for treatment A, 62.78% for treatment B, and 58.33% for treatment C. Mean Body Weight (MBW) values were recorded as 1.2592 g/shrimp for treatment A, 1.1216 g/shrimp for treatment B, and 0.7728 g/shrimp for treatment C. Average Daily Growth (ADG) results showed 0.0313 g/shrimp for treatment A, 0.0279 g/shrimp for treatment B, and 0.0192 g/shrimp for treatment C. Based on the study, the recommended stocking density for white vannamei shrimp is 40 shrimp/L, demonstrating improved growth and survival rates for this species.
Litopenaeus
Penaeidae
Shrimp farming
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Coupled reaction-chemiluminescence analysis is one of most effective means to broaden the application range of chemiluminescence analysis. In this paper, the development status on coupled reaction-chemiluminescence analysis was reviewed. These included enzyme-linked chemiluminescence analysis, chemiluminescence analysis with inorganic coupling reaction, chemiluminescence analysis with coupling photochemical reaction and chemiluminescence analysis with coupling electrochemical reaction.
Coupling reaction
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Historical Introduction
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Basic Principles of Chemiluminescence
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Classification of Chemiluminescent Reactions
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Methods in Chemiluminescence Research
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General Mechanisms in Organic Chemiluminescence
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High-Efficiency Organic Chemiluminescent Reactions Involving Peroxide Intermediates
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Acknowledgments
Keywords:
bioluminescence (BL) and chemiluminescence (CL);
high-energy intermediates (HEI);
chemiluminescence - basic principles;
chemiluminescent reaction classification;
physicochemical parameters in mechanistic CL research;
light emission intensity calibration;
highly efficient organic chemiluminescence systems;
back electron-transfer (BET) energetics
Peroxide
Organic peroxide
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