Mirella Di Lorenzo

University of Bath

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Pedro Estrela

University of Bath

Nikhil Bhalla

University of Ulster

Giordano Pula

Hull York Medical School

Benjamin Metcalfe

University of Bath

Petra J. Cameron

University of Bath

Thomas P. Curtis

Newcastle University

Jakub Dziegielowski

University of Bath

Domenico Pirozzi

University of Naples Federico II

Wanli Liu

University of Bath

Michele Mascia

University of Cagliari

Cooperative Institutions

University of Bath

Consejo Superior de Investigaciones Científicas

Universidad de Zaragoza

University of Salento

Newcastle University

University of Bristol

University of Cagliari

Engineering and Physical Sciences Research Council

Italian Institute of Technology

Center for Biomolecular Nanotechnologies

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Lipases in the oleochemical industry

Mirella Di Lorenzo Domenico Pirozzi Gianluca Greco

Source

Cite

Citations (0)

Generating power from transdermal extracts using a multi-electrode miniature enzymatic fuel cell

Biosensors and Bioelectronics (2015)

Hendrik du Toit Razleen Rashidi Dominic Ferdani M. Begoña Delgado‐Charro Carl M. Sangan

The development of self-powered wearable biodevices is highly attractive for a number of applications, such as health monitoring and drug delivery. Enzymatic fuel cells (EFCs) hold great potential as power sources for such devices, since they can generate power from physiological fluids and operate at body temperature. In this study, we present a cascade of three EFCs embedded in a compact and handy single channel device and we demonstrate for the first time power generation from iontophoresis extracts obtained from pig skin. The EFCs implement non-toxic highly-porous gold electrodes; an easy-to-reproduce procedure is adopted for the immobilization of glucose oxidase and laccase at the anode and cathode respectively; no external mediators are used; and the system design can easily be further miniaturized. When electrically connected in parallel, the EFCs generated a power output close to the sum of the power generated by each unit, with peak values of 0.7 µW (flow-through mode) and 0.4 µW (batch mode), at a glucose concentration of 27 mM. When the device was fed with transdermal extracts, containing only 30 μM of glucose, the average peak power was proportionally lower (0.004 µW).

Iontophoresis

Glucose oxidase

10.1016/j.bios.2015.11.074

Cite

Citations (25)

Self-Powered Detection of Glucose by Enzymatic Glucose/Oxygen Fuel Cells on Printed Circuit Boards

ACS Applied Materials & Interfaces (2021)

Carla Gonzalez-Solino Elena Bernalte Clara Bayona Richard Bennett Dónal Leech

Monitoring glucose levels in physiological fluids can help prevent severe complications associated with hypo- and hyper-glycemic events. Current glucose-monitoring systems require a three-electrode setup and a power source to function, which can hamper the system miniaturization to the patient discomfort. Enzymatic fuel cells (EFCs) offer the opportunity to develop self-powered and minimally invasive glucose sensors by eliminating the need for an external power source. Nevertheless, practical applications demand for cost-effective and mass-manufacturable EFCs compatible with integration strategies. In this study, we explore for the first time the use of gold electrodes on a printed circuit board (PCB) for the development of an EFC and demonstrate its application in saliva. To increase the specific surface area, the PCB gold-plated electrodes were modified with porous gold films. At the anode, glucose oxidase is immobilized with an osmium redox polymer that serves as an electron-transfer mediator. At the cathode, bilirubin oxidase is adsorbed onto the porous gold surface with a blocking agent that prevents parasitic reactions while maintaining the enzyme catalytic activity. The resulting EFC showed a linear response to glucose in phosphate buffer within the range 50 μM to 1 mM, with a sensitivity of 14.13 μA cm

Glucose oxidase

10.1021/acsami.1c02747

Cite

Citations (29)

Soil Microbial Fuel Cells for Energy Harvesting and Bioremediation of Soil Contaminated with Organic Pollutants

Byung Chul Kim Jakub Dziegielowski Mirella Di Lorenzo

Biostimulation

Bioaugmentation

10.1002/9781119762621.ch31

Cite

Citations (0)

Enzymatic Synthesis of Biodiesel

Chemical engineering transactions (2007)

Domenico Pirozzi Mirella Di Lorenzo L. Zuccaro Gianluca Greco

Lipases offer interesting perspectives as biocatalysts for the biodiesel synthesis, though their application is still limited by the poor stability of the enzyme at the operating conditions of industrial interest. In order to overcome this bottleneck, the inactivating effect of specific components of the methanolysis reacting system has been characterised, to develop a rational stabilization strategy. The experimental results have shown that the inactivating effect of the methanol can be avoided using long-chain alcohols as acyl acceptors, whereas the effect of glycerol is associated with diffusional limitations within the solid support used for the enzyme immobilisation.

Source

Cite

Citations (1)

Electrochemical deposition of CoNi micro/nanostructures as new materials for electrochemical sensing of glucose

Materials Letters (2015)

J. Vilana Mirella Di Lorenzo Elvira Gómez E. Vallés

Nanorod

Chronoamperometry

Deposition

Glassy carbon

10.1016/j.matlet.2015.06.116

Cite

Citations (26)

Engineering Functional Enzyme Bioelectrodes with Conductive Polymeric Films for Sensing Applications

Meeting abstracts/Meeting abstracts (Electrochemical Society. CD-ROM) (2019)

Carla Gonzalez-Solino Pawan Jolly Mirella Di Lorenzo

Immobilization of enzymes is a key strategy for the development of point-of care diagnostics such as biosensors and biofuel cells. Conductive polymeric films have been proposed as excellent signal transducers for biomedical applications due to their high electrical conductivity and biocompatibility. In this context, we report a highly innovative, while simple and robust, surface chemistry approach for the covalent immobilization of enzymes onto conductive polymeric nanostructures. In particular, an electrochemical grafting approach is proposed, according to which, polypyrrole (PPy) films on gold surfaces on-chip are modified with b-alanine (Ala) as a linker, bearing free carboxylate groups, for further probe immobilization. As a case study and considering its clinical relevance for glucose monitoring systems, the enzyme Glucose Oxidase (GOx) is used. The fabricated GOx/Ala/PPy/Au electrode exhibited excellent performance for glucose detection, with a linear response within the range of 0.1 mM - 10 mM, and a sensitivity of 3.75 µA mM -1 cm -2 . The observed low limit of detection is 0.1 mM; a value that lies below the normal glucose concentration in blood and non-conventional physiological fluids, such as interstitial fluid or saliva. The fabricated glucose sensor also demonstrates an excellent reproducibility with a relative standard deviation of 4 %. The methodology proposed paves the way for rapid manufacturing of effective enzyme-based polymeric electrodes, which, contrary to other PPy-based sensors previously suggested, does not involve any chemical pre-treatments of the monomer used. Figure 1

Polypyrrole

Biocompatibility

Glucose oxidase

Amperometry

Surface Modification

10.1149/ma2019-04/7/381

Cite

Citations (0)

Minimalistic soil microbial fuel cells for bioremediation of recalcitrant pollutants

E3S Web of Conferences (2022)

Arpita Nandy Byung Chul Kim Mirella Di Lorenzo

Increased human, agricultural and industrial activities along with improper waste disposal leads to high levels of soil contamination and accumulation of recalcitrant contaminants in the environment. This global issue demands the use of green and sustainable technologies and soil microbial fuel cells (SMFC) can be a potential solution. We adopted minimalistic designs, based on low-cost carbon materials without any expensive catalyst and membrane, which makes the SMFCs suitable for in-field applications. We investigated the ability of the indigenous microbial population of the soil to use organic contaminants as the source of carbon and the enrichment of the electroactive consortium was monitored over time onto the electrode surface of the SMFCs. We tested performance in soil contaminated with pesticide and soil contaminated with hydrocarbons and compare the microbial enrichment process with respect to the case of non-contaminated soil.

Soil Pollutants

10.1051/e3sconf/202233408004

Cite

Citations (2)

A flow‐through microfluidic chip for continuous dielectrophoretic separation of viable and non‐viable human T‐cells

Electrophoresis (2021)

Adil Mustafa Elisa Pedone Lucia Marucci Despina Moschou Mirella Di Lorenzo

Effective methods for rapid sorting of cells according to their viability are critical in T cells based therapies to prevent any risk to patients. In this context, we present a novel microfluidic device that continuously separates viable and non-viable T-cells according to their dielectric properties. A dielectrophoresis (DEP) force is generated by an array of castellated microelectrodes embedded into a microfluidic channel with a single inlet and two outlets; cells subjected to positive DEP forces are drawn toward the electrodes array and leave from the top outlet, those subjected to negative DEP forces are repelled away from the electrodes and leave from the bottom outlet. Computational fluid dynamics is used to predict the device separation efficacy, according to the applied alternative current (AC) frequency, at which the cells move from/to a negative/positive DEP region and the ionic strength of the suspension medium. The model is used to support the design of the operational conditions, confirming a separation efficiency, in terms of purity, of 96% under an applied AC frequency of 1.5 × 106 Hz and a flow rate of 20 μl/h. This work represents the first example of effective continuous sorting of viable and non-viable human T-cells in a single-inlet microfluidic chip, paving the way for lab-on-a-chip applications at the point of need.

Dielectrophoresis

Microchannel

Microfluidic chip

Lab-on-a-Chip

Suspension

10.1002/elps.202100031

Cite

Citations (12)

LAB-ON-PCB TECHNOLOGY FOR HANDHELD, SAMPLE-IN-ANSWER-OUT SARS-CoV-2 DIAGNOSTIC

Sotirios Papamatthaiou Weronika Witkowska McConnell Varun Kumar Sampath Kumar Uroš Zupancic Edward Lee-Emery

2019-20 coronavirus outbreak

Sample (material)

Source

Cite

Citations (0)