Carla Maleita

University of Coimbra

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Isabel Abrantes

University of Coimbra

Ivânia Esteves

University of Coimbra

R. H. C. Curtis

Rothamsted Research

Hermínio C. de Sousa

University of Coimbra

Duarte Santos

University of Coimbra

Mara E.M. Braga

University of Coimbra

Aida Maria Dâmaso Duarte

University of Idaho

Luís Fonseca

University of Coimbra

Joana M. S. Cardoso

University of Coimbra

Maria José da Cunha

Polytechnic Institute of Coimbra

Cooperative Institutions

University of Coimbra

Niğde Ömer Halisdemir Üniversitesi

University of Lisbon

Institute of Marine Research

Instituto Nacional de Investigação Agrária e Veterinária

Rothamsted Research

Consejo Superior de Investigaciones Científicas

Universidade Nova de Lisboa

Agricultural institute of Slovenia

University of Minho

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Molecular Characterization of Meloidogyne hispanica (Nematoda, Meloidogynidae) by Phylogenetic Analysis of Genes Within the rDNA in Meloidogyne spp.

Plant Disease (2008)

Blanca B. Landa Juan E. Palomares‐Rius N. Vovlas R. M. D. G. Carneiro Carla Maleita

In the past, the distribution of Meloidogyne hispanica, the Seville root-knot nematode, appeared to be restricted to the southern part of Spain and Prunus spp.; however, its distribution has been confirmed to be worldwide because it occurs in all continents (Europe, Africa, Asia, Australia, and North, Central, and South America). Differentiation of M. hispanica from other Meloidogyne spp., mainly M. arenaria, can be very difficult using morphological and biological traits data. These species are quite similar and can be regularly confused in inaccurate taxonomic comparisons. In this study, species-specific polymerase chain reaction (PCR) and phylogenetic analysis of sequences from three ribosomal (r)DNA regions (18S, internal transcribed spacer [ITS]1-5.8S-ITS2, and D2-D3 of 28S) were used to characterize three M. hispanica isolates from different geographical origins (Brazil, Portugal, and Spain). Molecular analyses showed identical sequences for all three isolates for the three rDNA regions. Maximum parsimony analysis of the three rDNA regions and the species-specific PCR demonstrated and supported the differentiation of M. hispanica from M. incognita, M. javanica, and M. arenaria and from all described root-knot nematode species.

Meloidogyne arenaria

10.1094/pdis-92-7-1104

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Citations (39)

In vitro co-culture of Solanum tuberosum hairy roots with Meloidogyne chitwoodi: structure, growth and production of volatiles

Plant Cell Tissue and Organ Culture (PCTOC) (2014)

Jorge M. S. Faria Inês Sena Carla Maleita Inês V. da Silva Lia Ascensão

Solanum tuberosum

Root-knot nematode

10.1007/s11240-014-0504-6

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Citations (10)

Characterization of the venom allergen—like protein (vap-1) and the fatty acid and retinol binding protein (far-1) genes in Meloidogyne hispanica

European Journal of Plant Pathology (2014)

Aida Maria Dâmaso Duarte R. H. C. Curtis Carla Maleita Igor Tiago Isabel Abrantes

10.1007/s10658-014-0436-3

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Citations (14)

Host status of cultivated plants to Meloidogyne hispanica

European Journal of Plant Pathology (2011)

Carla Maleita R. H. C. Curtis Stephen J. Powers Isabel Abrantes

The reproduction of a Meloidogyne hispanica isolate from Portugal was evaluated in 63 plant species/cultivars, in pot assays at 25 ± 2.0°C, on the basis of root gall index (GI) and reproduction factor (Rf = final/initial egg density) at 60 days after inoculation. Cultivars of aubergine, bean, beetroot, broccoli, carnation, corn, cucumber, French garlic, lettuce, melon, onion, parsley, pea, potato, spinach, and tobacco and two of cabbage were susceptible (3 ≤ GI ≤ 5; 1.15 ≤ Rf ≤ 262.86). Cabbage cv. Bacalan, cauliflower cv. Temporão and pepper cv. Zafiro R2 were hypersusceptible or poor hosts (Rf < 1; GI > 2) and pepper cvs. Aurelio and Solero were resistant (0.0 ≤ GI ≤ 0.4; 0.00 ≤ Rf ≤ 0.03). The response of the pepper cultivars and the Mi-1 resistant tomato cv. Rossol was also conducted in pots using two inoculum levels and four temperatures, three growth chamber (25 ± 2.7°C, 29.3 ± 1.8°C and 33.6 ± 1.2°C) and one outdoors (24.4 ± 8.2°C). At 24.4 ± 8.2°C and 25 ± 2.7°C, the reproduction on the resistant tomato was significantly lower compared to the susceptible cv. Easypeel. At all temperatures, resistance was evident for the pepper cultivars, despite the fact they were not found to contain any of the Me1, Me3, Me7 and N genes. The eggs obtained on cv. Aurelio at 33.6 ± 1.2°C were used to get a selected resistance breaking isolate of M. hispanica that was able to reproduce on the three pepper cultivars. Our results suggest that the initial M. hispanica isolate is a mixture of virulent and avirulent individuals. The pepper cultivars tested, have potential to reduce M. hispanica populations in agro-ecosystems under certain conditions, but they should be used as a part of an integrated management strategy in order to prevent the development of virulent populations.

Melon

10.1007/s10658-011-9918-8

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Juglone and 1,4-Naphthoquinone—Promising Nematicides for Sustainable Control of the Root Knot Nematode Meloidogyne luci

Frontiers in Plant Science (2022)

Carla Maleita Ivânia Esteves Mara E.M. Braga Joana Figueiredo Marisa C. Gaspar

The scarce availability of efficient and eco-friendly nematicides to control root-knot nematodes (RKN), Meloidogyne spp., has encouraged research toward the development of bionematicides. Naphthoquinones, juglone (JUG) and 1,4-naphthoquinone (1,4-NTQ), are being explored as alternatives to synthetic nematicides to control RKN. This study expands the knowledge on the effects of these natural compounds toward M. luci life cycle (mortality, hatching, penetration, reproduction). M. luci second-stage juveniles (J2)/eggs were exposed to each compound (250, 150, 100, 50, and 20 ppm) to monitor nematode mortality and hatching during 72 h and 15 days, respectively. Tomato seedlings were then inoculated with 200 J2, which had been exposed to JUG/1,4-NTQ for 3 days. The number of nematodes inside the roots was determined at 3 days after inoculation, and the final population density was assessed at 45 days after inoculation. Moreover, the potential mode of action of JUG/1,4-NTQ was investigated for the first time on RKN, through the assessment of reactive oxygen species (ROS) generation, acetylcholinesterase (AChE) in vitro inhibitory activity and expression analysis of ache and glutathione-S-transferase (gst) genes. 1,4-NTQ was the most active compound, causing ≥50% J2 mortality at 250 ppm, within 24 h. At 20 and 50 ppm, hatching was reduced by ≈50% for both compounds. JUG showed a greater effect on M. luci penetration and reproduction, decreasing infection by ≈80% (50 ppm) on tomato plants. However, 1,4-NTQ-induced generation of ROS and nematode vacuolization was observed. Our study confirms that JUG/1,4-NTQ are promising nematicidal compounds, and new knowledge on their physiological impacts on Meloidogyne was provided to open new avenues for the development of innovative sustainable nematicides.

Meloidogyne arenaria

Root-knot nematode

Juglone

10.3389/fpls.2022.867803

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Citations (10)

In vitro nematicidal activity of naphthoquinones against the root-lesion nematode Pratylenchus thornei

DOAJ (DOAJ: Directory of Open Access Journals) (2017)

Ivânia Esteves Carla Maleita Luís Fonseca Mara M.E. Braga Isabel Abrantes

The root-lesion nematode Pratylenchus thornei is a widely distributed and important parasite of cereals and legumes. As migratory endoparasites, P. thornei are difficult to manage because they enter and leave host roots and may remain quiescent inside roots or in soil when conditions are unfavourable for plant growth. The number of available nematicides to manage these nematodes is restricted, so new, effective and eco-friendly sustainable management strategies are needed. The effects of naphthoquinones (juglone, 1,4-naphtoquinone and plumbagin) produced by some plants species, including walnut (Juglandaceae), were assessed against P. thornei . An additional treatment of a mixture of juglone and 1,4-naphtoquinone (2:1, w/w), was included because these compounds are frequently found at these propotion in walnut extracts. Juveniles and adult nematodes were exposed to different concentrations of each naphthoquinone and nematode mortality was assessed. Juglone and 1,4-naphthoquinone (at 500 ppm) were more effective than plumbagin, and gave 100% mortality after 24 h of exposure. A synergistic effect was not detected when juglone and 1,4-naphthoquinone (2:1, w/w) were combined. Estimated lethal concentrations causing 50% P. thornei mortality (LC 50 s) (72 h exposure) were: 134.7 ppm for juglone, 161.2 ppm for 1,4-naphthoquinone, 207.6 ppm for juglone + 1,4-naphthoquinone (2:1, w/w), and 178.8 ppm for plumbagin. This study has demonstrated the nematicidal potential of these naphthoquinones against P. thornei , and has shown that walnut residues may be valuable sources for extraction of these compounds.

Juglone

Plumbagin

Juglandaceae

Naphthoquinone

1,4-Naphthoquinone

10.14601/phytopathol_mediterr-19314

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Citations (10)

Root-lesion and root-knot nematodes parasitizing potato

European Journal of Plant Pathology (2014)

Ivânia Esteves Carla Maleita Isabel Abrantes

Pratylenchus

Globodera rostochiensis

Globodera pallida

Pratylenchus penetrans

10.1007/s10658-014-0551-1

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Citations (25)

Molecular characterization of putative parasitism genes in the plant-parasitic nematode Meloidogyne hispanica

Journal of Helminthology (2014)

Aida Maria Dâmaso Duarte Carla Maleita Igor Tiago R. Curtis Isabel Abrantes

Abstract Meloidogyne hispanica ( Mhi ) is a difficult-to-control polyphagous root-knot nematode (RKN) species of emerging importance for economically valuable crops. Nematode secretions are likely to be the first signals perceived by the plant and are thought to be involved in various aspects of the plant–nematode interaction. The aims of this work were to identify and characterize M. hispanica parasitism genes: cathepsin L cysteine protease ( cpl-1 ), calreticulin ( crt-1 ), β-1,4-endoglucanase-1 ( eng-1 ) and manganese superoxide dismutase ( mnsod ). As there are no genomic data available for M. hispanica, primers were designed from the conserved regions of the putative parasitism genes in M. incognita and M. hapla and used to amplify the genes in M. hispanica, which led to the successful amplification of these genes in M. hispanica. Partial gene sequences were also obtained for M. arenaria , M. hapla , M. hispanica , M. incognita and M. javanica cpl-1 , crt-1 , eng-1 and mnsod genes, and their phylogenetic relationship analysed. In order to determine whether these genes are differentially expressed during M. hispanica development, cDNA was amplified from mRNA isolated from eggs, second-stage juveniles (J2) and females. Amplification products were observed from cDNA of all developmental stages for the Mhi-cpl-1 and Mhi-crt-1 genes. However, the gene Mhi-crt-1 exhibited intense amplification bands in females, while the Mhi-eng-1 gene was equally amplified in eggs and J2 and the Mhi-mnsod gene was only expressed in eggs. In comparison to the other RKN species, the genes Mhi-eng-1 and Mhi-mnsod showed transcription in different nematode developmental stages.

10.1017/s0022149x1400073x

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Citations (6)

Plant Parasitic Nematodes

MDPI eBooks (2022)

Carla Maleita Isabel Abrantes Ivânia Esteves

Plant-parasitic nematodes (PPNs) are economically important pests for numerous agriculture and forestry crops, representing a significant constraint on global food security and forestry health. Root knot nematodes (Meloidogyne spp.), potato cyst nematodes (Globodera spp.), and root lesion nematodes (Pratylenchus spp.) are some examples of PPNs that are ranked at the top in the list of the most economically and scientifically important species. Current approaches to controlling these PPNs include the use of nematicides, but many pose serious concerns for human health and the environment. To cope with such threats, accurate diagnostic methods for nematode detection and a deep understanding of nematode infection processes, as well as of their intricate relationships with the host plants, are crucial for the development of effective integrated nematode management programs. This Special Issue entitled “Pant Parasitic Nematodes” is a collection of 11 original papers that cover a wide range of topics, including the state of the art of important PPN, and the detection and management of PPNs through sustainable and eco-friendly strategies.

Pratylenchus

10.3390/books978-3-0365-5464-8

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Citations (3)

Bursaphelenchus xylophilus in Pinus sylvestris—The First Report in Europe

Forests (2024)

Luís Fonseca Hugo Silva Joana M. S. Cardoso Ivânia Esteves Carla Maleita

The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease and is considered an A2 quarantine organism by the European Plant Protection Organisation. In Europe, this nematode has been reported in Pinus pinaster, P. radiata, and P. nigra. In May 2024, severe wilting symptoms were observed in P. sylvestris trees at Serra da Lousã (Coimbra, the central area of continental Portugal). Wood samples were collected from six wilted trees, and the presence of PWN was investigated. From these, B. xylophilus specimens were detected in five out of the six trees. Species identification was performed based on species-specific morphological diagnostic characters, and this was confirmed by real-time PCR using species-specific primers targeting the B. xylophilus satellite DNA region. This study presents the first detection of B. xylophilus in P. sylvestris in Portugal and in Europe.

Xylophilus

10.3390/f15091556

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Citations (2)