Carla Maleita

University of Coimbra

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Isabel Abrantes

University of Coimbra

Ivânia Esteves

University of Coimbra

R. H. C. Curtis

Rothamsted Research

Hermínio C. de Sousa

University of Coimbra

Duarte Santos

University of Coimbra

Mara E.M. Braga

University of Coimbra

Aida Maria Dâmaso Duarte

University of Idaho

Luís Fonseca

University of Coimbra

Joana M. S. Cardoso

University of Coimbra

Maria José da Cunha

Polytechnic Institute of Coimbra

Cooperative Institutions

University of Coimbra

Niğde Ömer Halisdemir Üniversitesi

University of Lisbon

Institute of Marine Research

Instituto Nacional de Investigação Agrária e Veterinária

Rothamsted Research

Consejo Superior de Investigaciones Científicas

Universidade Nova de Lisboa

Agricultural institute of Slovenia

University of Minho

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Tomato root exudates induce transcriptional changes of Meloidogyne hispanica genes

DOAJ (DOAJ: Directory of Open Access Journals) (2015)

Aida Maria Dâmaso Duarte Carla Maleita Isabel Abrantes R. H. C. Curtis

Meloidogyne hispanica is a polyphagous root-knot nematode of emerging importance that has the ability to infect a broad range of plants, and tomato ( Solanum lycopersicum L.) crops can be severely damaged. This study investigated whether tomato root exudates regulate the expression of five candidate parasitism genes previously identified and sequenced in M. Hispanica . These were; calreticulin ( crt-1 ), cathepsin L cysteine protease ( cpl-1 ), β -1,4 endoglucanase-1 ( eng-1 ), fatty acid retinol binding protein ( far-1 ) and venom allergen-like protein ( vap-1 ). One thousand M. hispanica second-stage juveniles (J2) were exposed overnight to tomato root exudates, obtained from the root systems of 4-week-old plants during 4 h of agitation in sterilized distilled water, and the relative expression of the parasitism genes was determined by quantitative real-time PCR. The cpl-1 , crt-1 , far-1 and vap-1 genes were differentially up-regulated ( P <0.05) in the pre-parasitic J2 after exposure to tomato root exudates, while expression of eng-1 was largely unaffected ( P =0.05) by the treatment. This results suggests that tomato root exudates induce changes in the expression of candidate parasitism genes. Gene silencing by RNAi will elucidate the exact function of these candidate parasitism genes in nematode penetration and survival. Identification of the plant signal molecules in the tomato root exudates responsible for the up-regulation of these parasitism genes may lead to the development of novel approaches for the management of M. hispanica .

Nematode infection

Root-knot nematode

Cysteine protease

Tobacco rattle virus

Candidate gene

10.14601/phytopathol_mediterr-14595

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Effect of the Mi gene on reproduction of Meloidogyne hispanica on tomato genotypes

Nematology (2011)

M. Clara Vieira dos Santos R. H. C. Curtis Isabel Abrantes Carla Maleita Stephen J. Powers

Abstract The root-knot nematode resistance ( Mi ) gene was screened in 25 tomato genotypes of Solanum lycopersicum , by amplification of REX-1 and Mi23 markers. Ten heterozygous tomato genotypes (Mimi), nine homozygous (MiMi) at the Mi locus and six lacking the Mi gene for resistance to root-knot nematode were identified using the marker REX-1. The results obtained with Mi23 marker confirmed the Mi gene status of the tomato genotypes, except for genotype Valouro RZ F1 that was homozygous (MiMi) and heterozygous (Mimi) at the Mi locus when using the REX-1 and Mi23 markers, respectively. The pathogenicity of Meloidogyne hispanica on the 25 tomato genotypes was assessed 60 days after inoculation with 5000 eggs on the basis of root gall index (GI) and reproduction factor (Rf). All the tomato genotypes were susceptible (excellent or good hosts), with GI > 4 and Rf > 2, except the genotype Rapit (Mimi), considered as resistant/hypersensitive (poor host). In this genotype, the nematode induced galls (GI = 4) on its roots and a small number of eggs were produced (Pf = 3085 ± 485). Significant differences in reproduction were detected between the Mi allelic conditions and genotypes within Mi allelic conditions. The increasing number of Mi alleles (0, 1 or 2) is associated with decreasing Rf, which suggests a possible dosage effect of the Mi gene. The variability observed in the Rf values for MiMi tomato genotypes may reflect an influence of the genetic background of the plants containing the Mi gene. Ten of the 25 tomato genotypes with Mi gene are commercially available. However, only Rapit can be used to control the three most common Meloidogyne spp. and inhibit the increasing of M. hispanica populations, and may have potential to be included in an integrated pest management programme. However, it is advisable to evaluate the pathogenicity of local populations of this nematode species associated with different environmental factors.

Root-knot nematode

10.1163/138855411x566449

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Species-Specific Molecular Detection of the Root Knot Nematode Meloidogyne luci

Biology (2021)

Carla Maleita Joana M. S. Cardoso Leidy Rusinque Ivânia Esteves Isabel Abrantes

Meloidogyne luci has been identified in various countries around the world parasitizing economically important crops and, due to its potential to cause serious damage to agriculture, was included in the European and Mediterranean Plant Protection Organization Alert List in 2017. This species shares morphological and molecular similarities with M. ethiopica and M. inornata, and a M. ethiopica group was therefore established. Although specific primers for the DNA amplification of species belonging to the M. ethiopica group have been developed previously, the primers were not species-specific, so molecular markers for the specific detection of M. luci are still needed. The objective of this study was to develop a SCAR marker for the detection of M. luci and the discrimination from other Meloidogyne spp. based on the intraspecific variability found in RAPD markers. RAPD screening of M. luci and M. ethiopica genome was used for the identification of a specific amplification product on M. luci, which was cloned, sequenced and converted into a SCAR marker. The specificity of the designed primers (Mlf/r) was tested and produced a fragment (771 bp) for all nine M. luci isolates with no amplification for the other nine Meloidogyne spp., including M. ethiopica and M. inornata. Additionally, the proper amplification of the M. luci SCAR-marker was also successful with DNA from galls of M. luci infected tomato roots. The results obtained in this study reveal that the specific molecular detection of M. luci was achieved and that the developed methodology can be used for routine diagnosis purposes, which are essential to monitoring the distribution and spread of M. luci in order to implement future effective and integrated nematode pest management programs.

Molecular marker

10.3390/biology10080775

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New Hosts and Records in Portugal for the Root-Knot Nematode Meloidogyne luci

Journal of Nematology (2019)

Duarte Santos António Ferrer Correia Isabel Abrantes Carla Maleita

Sciendo provides publishing services and solutions to academic and professional organizations and individual authors. We publish journals, books, conference proceedings and a variety of other publications.

Root-knot nematode

10.21307/jofnem-2019-003

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Meloidogyne luci, a new root‐knot nematode parasitizing potato in Portugal

Plant Pathology (2017)

Carla Maleita Ivânia Esteves Joana M. S. Cardoso Maria José da Cunha R. M. D. G. Carneiro

In 2013, during a field survey conducted in Portugal on potato, Solanum tuberosum , an unusual esterase ( EST ) phenotype was detected in a root‐knot nematode ( RKN ) from potato roots collected in Coimbra. This Portuguese isolate was purified and maintained on tomato, S. lycopersicum , and morphological, biochemical and molecular characteristics were studied. Perineal pattern morphology was highly variable, similar to Meloidogyne ethiopica and not useful for identification. The EST phenotype, from young egg‐laying females, displayed three bands similar to the Brazilian M. luci (L3) and distinct from M. ethiopica (E3). Phylogenetic analyses of mitochondrial cytochrome oxidase subunit I and the mitochondrial DNA region between COII and 16S rRNA genes revealed that the Portuguese isolate grouped with M. luci isolates close to M. ethiopica isolates. However, considering the ITS 1‐5.8S‐ ITS 2 region, the Portuguese isolate grouped with isolates of M. luci , M. ethiopica and M. hispanica , which limits the confidence of this region for M. luci diagnosis, and its differentiation from other species with morphological similarities. The M. luci pathogenicity to potato was also assessed in 16 commercial cultivars and compared with M. chitwoodi , considered to be a quarantine RKN species by EPPO . All potato cultivars were susceptible to both Meloidogyne species with gall indices of 5 and higher reproduction factor values ranging from 12.5 to 122.3, which suggests that M. luci may constitute a potential threat to potato production. In the present study, M. luci is reported for the first time attacking potato in Portugal.

Root-knot nematode

10.1111/ppa.12755

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Citations (38)

Editorial: Sustainable strategies for the management of phytoparasitic nematodes

Frontiers in Plant Science (2023)

Carla Maleita Ivânia Esteves Aurelio Ciancio Yuji Oka

EDITORIAL article Front. Plant Sci., 31 January 2023Sec. Plant Pathogen Interactions Volume 14 - 2023 | https://doi.org/10.3389/fpls.2023.1148261

10.3389/fpls.2023.1148261

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Nematodes associated with cork oak, Quercus suber, in Montado ecosystems

Sofia R. Costa Ana Martins María L. Arosa Helena Freitas M. T. M. Almeida

Quercus suber

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Influence of temperature on the life cycle of the root-knot nematode Meloidogyne hispanica

Carla Maleita M. Clara Vieira dos Santos R. H. C. Curtis Isabel Abrantes

Root-knot nematode

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In vitro reproduction of Pratylenchus neglectus on carrot discs and investigation of its interaction with Meloidogyne hispanica on potato

European Journal of Plant Pathology (2018)

Ivânia Esteves Carla Maleita Tânia dos Milagres Pato Isabel Abrantes Maria José da Cunha

Solanum tuberosum

Pratylenchus

Potato cyst nematode

Population density

10.1007/s10658-018-1582-9

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First report of Laimaphelenchus heidelbergi (Nematoda: Aphelenchoididae) in Europe

Forest Pathology (2014)

Carla Maleita Sofia R. Costa Isabel Abrantes

Summary A survey of nematodes associated with branches of cork oak, Quercus suber , a species in decline since the second half of the 20th century, was conducted on two farms located in Alentejo, Portugal. Using specific morphological characters, some nematodes were identified as belonging to the genus Laimaphelenchus and one of the isolates being identified as L. heidelbergi . This research aimed to characterize the Portuguese L. heidelbergi isolate using morphobiometrical and molecular analyses and to analyze its phylogenetic relationship to other Laimaphelenchus spp. Morphometric and morphological characteristics of L. heidelbergi females and males were similar to the original description. For molecular analyses, the mitochondrial DNA region from the cytochrome oxidase subunit I and the D2/D3 expansion segments of the large subunit of rDNA were amplified and sequenced. In phylogenetic analyses, sequences of the Portuguese L. heidelbergi isolate clustered with sequences from the Australian isolate. Laimaphelenchus heidelbergi was originally described from wood of Pinus radiata growing in Australia and is here reported for the first time in Europe and Portugal. Cork oak is a new host record for L. heidelbergi .

Quercus suber

10.1111/efp.12120

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Citations (7)