Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia.Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity.Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037).Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.
AbstractKecacingan merupakan masalah kesehatan yang masih banyak di temukan di dunia. Prevalensi kecacingan di Indonesia berdasarkan angka nasional (28,12%). Sumatera barat (82,3%) dengan rincian prevalensi cacing ascaris lumbricodies 17,75%, cacing Trichuris trichiura 17,74% dan cacing Hookworm 6,46. Tujuan: Mengetahui faktor penyebab kejadian kecacingan pada anak SD diwilayah kerja Puskesmas X Padang. Metode: Penelitian ini menggunakan metode kuantitatif dengan desain cross sectional dua variabel (independen dan dependen). Variabel independen: tingkat pendidikan dan pengetahuan ibu, kebiasaan anggota keluarga berdefikasi, kebersihan kuku anak, status ekonomi keluarga, keadaan lantai rumah dan kebersihan lingkungan dengan menggunakan kuesioner. Variabel dependen: Hasil pemeriksaan feses secara langsung menggunakan pewarnaan eosin 2 % secara mikroskopis. Hasil: penelitian ini menunjukan bahwa kejadian kecacingan pada anak SD sebesar 53,2 %, tingkat pendidikan ibu tinggi 54,0 %, pengetahuan ibu rendah 73,4 %, Kebiasaan anggota keluarga berdefikasi baik 96,8%, kebersihan kuku baik 64,5 %, status ekonomi menengah 75 %, keadaan lantai rumah baik 74,2 %, kebersihan lingkungan buruk 62,9 %. Simpulan: Kejadian kecacingan pada anak SD masih tinggi, implementasi kebijakan program kecacingan di puskesmas belum maksimal.
Ikan lele merupakan salah satu dari ikan yang banyak di budidayakan di Indonesia karena proses pemeliharaannya relatif sederhana. Selama metabolisme protein organisme air, amonia biasanya diproduksi sebagai hasil ekskresi. Amonia (NH3) merupakan senyawa yang mengandung Nitrogen dan Hidrogen, kadar amonia bisa menyebabkan terjadinya pencemaran yang dapat menurunkan kualitas air sungai. Penelitian ini bertujuan untuk mengetahui penurunan kadar amonia (NH3) dengan penambahan bakteri Rhodobacter dan Ekoenzim pada air kolam ikan lele. Analisis data pada penelitian ini menggunakan data deskriptif kualitatif. Penelitian menggunakan metode analisis data secara deskriptif, kualitatif. Analisis data bersifat induktif yaitu salah satu analisis berdasarkan data yang akan diperoleh, selanjutnya dikembangkan menjadi hipotesis. Pada penelitian ini dijelaskan secara deskriptif. Kemudian diuji kemampuan bioremediasinya meliputi presentasi kehilangan ammonia dan kekeruhan air, dengan pengamatan 0, 2, 4, 6, 8 hari dan 3 kali pengulangan. Penelitian kualitatif dilakukan dengan menguji kadar amonia menggunakan metode SNI 06-6989.30-2005. Hasil penelitian ini menunjukkan bahwa penambahan bakteri Rhodobacter pada air kolam ikan berpotensi menurunkan kadar amonia (NH3), dengan kadar amonia (NH3) awal 12,6 mg/l dengan adanya penambahan bakteri Rhodobacter terjadi penurunan amonia (NH3) sebesar 9,4 mg/l.
<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><em>One House One Larva Monitor Movement is a dengue vector control program of the Ministry of Health which has been promoted since 2016. However, there are still several areas that have not been successful in implementing this movement, one of which includes the City of Padang. The purpose of this study was to examine the importance of using digital applications in implementing dengue vector control programs. This research is a research with qualitative methods using the case study genre through a constructivist approach. The location of the qualitative research was carried out at the Padang City Health Office, in the working areas of the Nanggalo Health Center, Pagambiran Health Center, and Rawang Health Center which were designated as research areas. This research was conducted in 2023 with informants including the person in charge of the dengue fever program at the Padang City Health Service, the person in charge of the dengue fever program at the Community Health Center, and the larva monitoring coordinator. The results of the study indicate that efforts to control dengue vectors through the One House One larva monitor Movement are still not running as they should. This movement can only run when larva monitor performs its main tasks and functions in accordance with the provisions. For this reason, it is necessary to carry out coaching and supervision efforts. However, there are several obstacles that require the design of a comprehensive and integrated digital health application to improve the health information system to improve the performance of larva monitors.</em></p><p><em> </em></p></td></tr></tbody></table></div><p><strong><em>Keyword: DHF, Digital Health, Vector Dengue</em></strong></p>
Background: Non-communicable diseases (NCD) are the leading cause of death globally today. In West Sumatra there has been an increase in NCD which exceeds the national average percentage. Telomere shortening is associated with occurrence of NCD. Shortening of telomeres is influenced by diet. The purpose of this study was to examine the relationship between Saturated Fatty Acid (SAFA) intake and Superoxide Dismutase (SOD) activity with the telomere length of Minangkabau ethnic men.Methods: This study was a cross sectional study. The sample of this study were 69 civil servants of Minangkabau male at district office in the Padang City, aged 40-50 years and according to the inclusion and exclusion criteria. Data on SAFA intake was obtained using Semi Quantitative Food Frequency Questionnaire (SQFFQ), SOD activity was measured using the Calorimetric method and telomere length was measured using Real Time Polymerase Chain Reaction (RT-PCR) by O’Challaghan and Fennech method. Data analysis was performed using Pearson correlation test with significance level p <0.05.Results: The results showed an average SAFA intake 10.47+3.46% calories, SOD activity 5.93+0.81units/ml and telomere length 468.94+199.25bp. Correlation test between telomere length with SAFA intake showed (p=0.338), and the correlation test between SOD activity and telomere length showed (p=0.01).Conclusions: From this study it was concluded that there was no correlation between SAFA intake and telomere length and there was a significant positive correlation between SOD activity and the telomere length of Minangkabau ethnic men.
Background. Premenopause is a transition period from reproduction to menopause. This phase is characterized by a decrease in estradiol levels which causes body fat mass and cholesterol levels tend to rise in most women of premenopausal age.
Objective. This study aims to determine the relationship of body fat mass and cholesterol levels with serum estradiol levels in premenopausal women in Padang city.
Method. This study is an analytic study witha cross sectional study design. This study involved 112 data of premenopausal female respondents aged 40-55 years in the city of Padang, which consisted of data on age, occupation, body fat mass, total cholesterol levels and estradiol levels. Data obtained from observation of the master table. Analysis of the relationship of body fat mass and cholesterol levels with estradiol levels was using the One Way Anova test.
Result. The results showed the mean body fat mass of premenopausal women in Padang City was 37.3±7.2%, the mean cholesterol level was 204.45±40.73 mg/dL and the average estradiol level was 145.72±49.82 pg/ml. Based on the results of the analasis test, a significant negative correlation was obtained, p=0.026 (p<0.05) between cholesterol levels with estradiol levels. While there is no relationship between body fat mass with estradiol levels p=0.714 (p>0.05).
Conclusion. There is a relationship between cholesterol levels with estradiol levels, but there is no relationship between body fat mass with serum estradiol levels of premenopausal women in Padang City.
<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><em>Deviations in sexual behavior often occur among adolescents. The development of the current era makes information disclosure through media and electronics have a major influence on adolescent sexual behavior. The research objective was to determine the factors influencing risky actions on the reproductive health of early adolescents at public junior high school 30 Kerinci. This type of quantitative analytic research with a cross-sectional design approach. The research was conducted in November - December 2022 at public junior high school 30 Kerinci with a total sample of 77 people. The sampling technique for this research is total sampling. Data collection using a questionnaire. Analysis of the research data with the chi-square statistical test and multivariate analysis with the binary logistic test. The results showed that there was a relationship between knowledge and risky actions for reproductive health (p=0.001), there was a relationship between attitude and risky actions for reproductive health (p=0.039), there was a relationship between the role of parents and risky actions for reproductive health (p=0.014), there was no relationship between the role of health workers and reproductive health risky actions (p=0.647), there is a relationship between the role of peers and reproductive health risky actions (p=0.027), the dominant factor that has the most influence on reproductive health risky actions in early adolescents in junior high school 30 Kerinci is the knowledge factor (p-value=0.001; POR=16.619). It is suggested that the school activate youth care activity services activities at schools in cooperation with local health workers. I</em><em>t’s</em><em> also suggested that health workers provide health education or youth health seminars at schools to provide information about reproductive health for adolescents. Furthermore, it is suggested to parents of students to control adolescents so that adolescents are correct in receiving health information, especially reproductive health, and seeking information related to adolescent reproductive health together. </em></p><p><em> </em></p></td></tr></tbody></table></div><p><strong><em>Keywords : Adolescents, Risky Action , Reproductive Health</em></strong></p>
Background: Telomere length (TL) is known as the best aging biomarker to date. Oxidative stress is one of the factors that affect TL. The process of macronutrient metabolism will produce free radicals in the body, so antioxidants are needed to maintain telomere length.Methods: A cross-sectional study was conducted in Padang City on 239 samples obtained by multi-stage random sampling after signing informed consent. Characteristic data were obtained through interviews using a questionnaire and intake of macronutrients and intake of antioxidants using the FFQ. Examination of telomere length using a modified method of O'Callaghan and Fenech. The data is processed computerized, and the statistical analysis results are considered significant if the p-value is 0.05.Findings: The average telomere length is 565.39 + 120.83 bp. The intake of Energy, Carbohydrates, and Total Fat is in accordance with the Indonesian people's nutritional adequacy rate (RDA), while SAFA intake is higher and PUFA and MUFA intake is lower than the RDA. There was a significant correlation between intake of Energy, Total Fat, SAFA, PUFA and MUFA with telomere length (p < 0,05). The average intake of vitamins A and vitamin C is higher than the RDA, and other antioxidants such as Vitamin E, Selenium, and Zinc are lower than the RDA. There is no correlation between the intake of Vitamin A, Vitamin C, Vitamin E and, selenium with telomere length, but there is a significant correlation between Zinc intake and telomere length where the higher the intake of Zinc, the longer the telomere of research subjects.Interpretation: Energy intake, Total Fat, SAFA, PUFA, and MUFA are risk factors for telomere length, while Zinc intake is a protective factor against telomere shorteningFunding: No.Declaration of Interest: We declare no conflicts of interest.Ethical Approval: Research approval was obtained from the health and research ethics committee, Faculty of Medicine, University of Andalas No. 448/UN.16.2/KEP-FK/2023. Samples obtained by multi-stage random sampling after signing informed consent.
Latar Belakang: Kanker payudara merupakan jenis kanker dengan angka kejadiannya menempati posisi pertama terbanyak di Indonesia dibandingkan dengan jenis kanker lain. Daun sirsak yang mengandung acetogenin memiliki efek sitotoksik terhadap sel kanker payudara T47D.
Objektif: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun sirsak terhadap level ekspresi gen p53 sel kanker payudara T47D secara in vitro.
Metode: Penelitian ini merupakan penelitian eksperimental dengan metode kuantifikasi relatif real time PCR menggunakan cell line T47D diinkubasi selama 72 jam yang dibagi menjadi 2 kelompok sampel, yaitu perlakuan dan kontrol. Kelompok perlakuan diberikan larutan uji ekstrak etanol daun sirsak dengan konsentrasi IC50 = 94,26 µg/ml.
Hasil: Hasil nilai Cq dari gen target dan referensi dianalisis dengan menggunakan uji Mann Whitney dan rumus livak. Hasil penelitian didapatkan perbedaan yang bermakna antara kelompok perlakuan dan kontrol dengan nilai p = 0,003 (p < 0,05). Analisis dengan rumus livak menunjukkan adanya peningkatan ekspresi gen p53 sel kanker payudara T47D yang diberi perlakuan, yaitu 2,8015 kali lipat lebih tinggi dari sel kelompok kontrol.
Kesimpulan: Ekstrak daun sirsak efektif dapat meningkatkan ekspresi gen p53 pada cell line T47D dan terdapat perbedaan yang bermakna dari nilai Cq gen p53 sel kelompok perlakuan dan kontrol.
The prevalence of caries among school children in West Sumatera is still high. Dental health education is important in order to increase dental health status in children because of that the development of a new strategy in dental health education is needed. One of the way is by using game, because children usually like that media. This study was aimed to find out the effectiveness of dental education game by using snake and ladder as the media in changing 7-8years old student’s knowledge about teeth and mouth. The method of this study was analytic experimental with non equivalent pre and post test control group design. Fourty one student's with 7-8 years old of two Elementary Schools ( 17 Manggis Ganting and 03 Pulai Anak Air elementary school ) were used as the study sample of experiment group, this group was given dental
education games method with snake and ladder games as the media and fourty one student's with 7-8 years old of 17 Manggis Ganting and 03 Pulai Anak Air elementary school were used as the control group where in this group was given nothing. Analysis of data was using Paired Samples T-Test and Independent Samples T-Test based on 0,05 as significant. The pretest and posttest analysis of knowledge showed that mean percentage of experiment group is 13,32% ±12,475 and 4,44% ±11,713 for the control group. Both of groups have significant difference where p=0,00(p<0,05). Dental education games method with snake and ladder games as media was effective in increasing student’s knowledge in comparing with the control group. So in order to increase dental health knowledge, dental education games could be use as one of dental education method for 7-8 years old students.
