Beatriz Pelaz

Center for Research in Molecular Medicine and Chronic Diseases

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Pablo del Pino

Center for Research in Molecular Medicine and Chronic Diseases

Wolfgang J. Parak

Universität Hamburg

Ester Polo

Universidade de Santiago de Compostela

Mahmoud G. Soliman

Royal College of Surgeons in Ireland

Jesús M. de la Fuente

Universidad de Zaragoza

Karsten Kantner

Philipps University of Marburg

Neus Feliu

Universität Hamburg

Carolina Carrillo‐Carrión

Universidad de Sevilla

Daniel Valdepérez

Philipps University of Marburg

Pablo Taboada

Universidade de Santiago de Compostela

Cooperative Institutions

Universidade de Santiago de Compostela

Philipps University of Marburg

Centre National de la Recherche Scientifique

Instituto de Investigación Sanitaria de Santiago

Center for Research in Molecular Medicine and Chronic Diseases

Consejo Superior de Investigaciones Científicas

Universidad de Zaragoza

Chinese Academy of Sciences

Instituto de Salud Carlos III

University of Florida

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Synthesis and Surface Engineering of Gold Nanoparticles, and Their Potential Applications in Bionanotechnology

Elsevier eBooks (2017)

Mahmoud G. Soliman Pablo del Pino Wolfgang J. Parak Beatriz Pelaz

Nanobiotechnology

Surface Engineering

10.1016/b978-0-12-409547-2.12744-1

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Citations (3)

How Entanglement of Different Physicochemical Properties Complicates the Prediction of in Vitro and in Vivo Interactions of Gold Nanoparticles

Ming Xu Mahmoud G. Soliman Xing Sun Beatriz Pelaz Neus Feliu

The physicochemical properties of a set of 21 different gold nanoparticles (spherical and rod-shaped nanoparticles (NPs) of different diameters with three different surface coatings) were studied. Protein corona formation, in vitro uptake, effect on cell viability and proliferation, and in vivo biodistribution of these NPs were determined. The relation of the results of the different NPs was analyzed by hierarchical cluster analysis, which will tell which NPs have the most similar physicochemical properties and biological effects, without having to specify individual physicochemical parameters.

10.1201/9781003306498-30

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Citations (3)

High-Content Imaging and Gene Expression Approaches To Unravel the Effect of Surface Functionality on Cellular Interactions of Silver Nanoparticles

ACS Nano (2015)

Bella B. Manshian Christian Pfeiffer Beatriz Pelaz Thomas Heimerl Marta Gallego

The toxic effects of Ag nanoparticles (NPs) remain an issue of debate, where the respective contribution of the NPs themselves and of free Ag(+) ions present in the NP stock suspensions and after intracellular NP corrosion are not fully understood. Here, we employ a recently set up methodology based on high-content (HC) imaging combined with high-content gene expression studies to examine the interaction of three types of Ag NPs with identical core sizes, but coated with either mercaptoundecanoic acid (MUA), dodecylamine-modified poly(isobutylene-alt-maleic anhydride) (PMA), or poly(ethylene glycol) (PEG)-conjugated PMA with two types of cultured cells (primary human umbilical vein endothelial cells (HUVEC) and murine C17.2 neural progenitor cells). As a control, cells were also exposed to free Ag(+) ions at the same concentration as present in the respective Ag NP stock suspensions. The data reveal clear effects of the NP surface properties on cellular interactions. PEGylation of the NPs significantly reduces their cellular uptake efficiency, whereas MUA-NPs are more prone to agglomeration in complex tissue culture media. PEG-NPs display the lowest levels of toxicity, which is in line with their reduced cell uptake. MUA-NPs display the highest levels of toxicity, caused by autophagy, cell membrane damage, mitochondrial damage, and cytoskeletal deformations. At similar intracellular NP levels, PEG-NPs induce the highest levels of reactive oxygen species (ROS), but do not affect the cell cytoskeleton, in contrast to MUA- and PMA-NPs. Gene expression studies support the findings above, defining autophagy and cell membrane damage-related necrosis as main toxicity pathways. Additionally, immunotoxicity, DNA damage responses, and hypoxia-like toxicity were observed for PMA- and especially MUA-NPs. Together, these data reveal that Ag(+) ions do contribute to Ag NP-associated toxicity, particularly upon intracellular degradation. The different surface properties of the NPs however result in distinct toxicity profiles for the three NPs, indicating clear NP-associated effects.

Silver nanoparticle

10.1021/acsnano.5b04661

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Citations (77)

Protein Corona Formation around Nanoparticles—From the Past to the Future*

Pablo del Pino Beatriz Pelaz Qian Zhang Pauline Maffre G. Ulrich Nienhaus

The protein adsorption layer (a.k.a. the "protein corona") that forms on the surface of colloidal nanoparticles plays an important role in their interaction with living matter. Thus, characterization of the protein corona is of utmost importance for understanding how exposure to nanoparticles affects the biological responses of cells and organisms. Although a lot of experimental studies have been reported in this direction, a comprehensive picture is still missing, in particular due to the multitude of different scenarios under which experiments have been performed. In this review an analysis of existing experimental data about the protein corona, and an outline for required future work will be given. In particular we review how existing simple analytical models such as the adopted Hill model may help to extract quantitative data from such experiments such as equilibrium dissociation and kinetic coefficients. Careful quantitative assessment of equilibrium and kinetic properties would allow for a comparison of protein binding data from the vast array of engineered nanoparticles, so that basic principles could be revealed. This review outlines that the field is in dire need of more quantitative studies to further our understanding of protein corona formation and its biological consequences.

Corona (planetary geology)

10.1201/9781003306498-25

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Cellular uptake and bioactivity of antibody-gold nanoparticle bioconjugates

Journal of Biotechnology (2016)

Gamze Tan Karsten Kantner Qian Zhang Mahmoud G. Soliman Pablo del Pino

Emission intensity

10.1016/j.jbiotec.2016.05.121

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Ultrafast synthesis of zirconium-porphyrin framework nanocrystals from alkoxide precursors

Cell Reports Physical Science (2024)

Manuel Ceballos Giulia Zampini Oleg Semyonov Samuel Funes‐Hernando José Manuel Vila‐Fungueiriño

Alkoxide

10.1016/j.xcrp.2024.102318

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Citations (1)

Basic Physicochemical Properties of Polyethylene Glycol Coated Gold Nanoparticles that Determine Their Interaction with Cells

Angewandte Chemie (2016)

Pablo del Pino Fang Yang Beatriz Pelaz Qian Zhang Karsten Kantner

Abstract A homologous nanoparticle library was synthesized in which gold nanoparticles were coated with polyethylene glycol, whereby the diameter of the gold cores, as well as the thickness of the shell of polyethylene glycol, was varied. Basic physicochemical parameters of this two‐dimensional nanoparticle library, such as size, ζ‐potential, hydrophilicity, elasticity, and catalytic activity ,were determined. Cell uptake of selected nanoparticles with equal size yet varying thickness of the polymer shell and their effect on basic structural and functional cell parameters was determined. Data indicates that thinner, more hydrophilic coatings, combined with the partial functionalization with quaternary ammonium cations, result in a more efficient uptake, which relates to significant effects on structural and functional cell parameters.

Surface Modification

10.1002/ange.201511733

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Citations (16)

Protein corona formation around nanoparticles – from the past to the future

Materials Horizons (2013)

Pablo del Pino Beatriz Pelaz Qian Zhang Pauline Maffre G. Ulrich Nienhaus

The protein adsorption layer (a.k.a. the “protein corona”) that forms on the surface of colloidal nanoparticles plays an important role in their interaction with living matter. Thus, characterization of the protein corona is of utmost importance for understanding how exposure to nanoparticles affects the biological responses of cells and organisms. Although a lot of experimental studies have been reported in this direction, a comprehensive picture is still missing, in particular due to the multitude of different scenarios under which experiments have been performed. In this review an analysis of existing experimental data about the protein corona, and an outline for required future work will be given. In particular we review how existing simple analytical models such as the adopted Hill model may help to extract quantitative data from such experiments such as equilibrium dissociation and kinetic coefficients. Careful quantitative assessment of equilibrium and kinetic properties would allow for a comparison of protein binding data from the vast array of engineered nanoparticles, so that basic principles could be revealed. This review outlines that the field is in dire need of more quantitative studies to further our understanding of protein corona formation and its biological consequences.

Corona (planetary geology)

Characterization

10.1039/c3mh00106g

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Citations (515)

Colloidal Gold Nanoparticles Induce Changes in Cellular and Subcellular Morphology

ACS Nano (2017)

Xiaowei Ma Raimo Hartmann Dorleta Jiménez de Aberasturi Fang Yang Stefaan J. Soenen

Exposure of cells to colloidal nanoparticles (NPs) can have concentration-dependent harmful effects. Mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e., viability assays, gene expression profiles, etc., neglecting that the presence of NPs can also drastically affect cellular morphology. In the case of polymer-coated Au NPs, we demonstrate that upon NP internalization, cells undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances in actin and tubulin cytoskeleton and associated signaling, and reduction of focal adhesion contact area and number of filopodia. Appropriate imaging and data treatment techniques allow for quantitative analyses of these concentration-dependent changes. Abnormalities in morphology occur at similar (or even lower) NP concentrations as the onset of reduced cellular viability. Cellular morphology is thus an important quantitative indicator to verify harmful effects of NPs to cells, without requiring biochemical assays, but relying on appropriate staining and imaging techniques.

Filopodia

Internalization

Viability assay

Morphology

10.1021/acsnano.7b01760

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Citations (98)

Magnetic Nanoparticles for Cancer Therapy and Bioimaging

Nanomedicine and nanotoxicology (2018)

Ester Polo Pablo del Pino Alberto Pardo Pablo Taboada Beatriz Pelaz

Nanomaterials

Cancer Therapy

Cancer Treatment

10.1007/978-3-319-89878-0_7

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Citations (15)