High-Content Imaging and Gene Expression Approaches To Unravel the Effect of Surface Functionality on Cellular Interactions of Silver Nanoparticles
Bella B. ManshianChristian PfeifferBeatriz PelazThomas HeimerlMarta GallegoMarco MöllerPablo del PinoUwe HimmelreichWolfgang J. ParakStefaan J. Soenen
77
Citation
65
Reference
10
Related Paper
Citation Trend
Abstract:
The toxic effects of Ag nanoparticles (NPs) remain an issue of debate, where the respective contribution of the NPs themselves and of free Ag(+) ions present in the NP stock suspensions and after intracellular NP corrosion are not fully understood. Here, we employ a recently set up methodology based on high-content (HC) imaging combined with high-content gene expression studies to examine the interaction of three types of Ag NPs with identical core sizes, but coated with either mercaptoundecanoic acid (MUA), dodecylamine-modified poly(isobutylene-alt-maleic anhydride) (PMA), or poly(ethylene glycol) (PEG)-conjugated PMA with two types of cultured cells (primary human umbilical vein endothelial cells (HUVEC) and murine C17.2 neural progenitor cells). As a control, cells were also exposed to free Ag(+) ions at the same concentration as present in the respective Ag NP stock suspensions. The data reveal clear effects of the NP surface properties on cellular interactions. PEGylation of the NPs significantly reduces their cellular uptake efficiency, whereas MUA-NPs are more prone to agglomeration in complex tissue culture media. PEG-NPs display the lowest levels of toxicity, which is in line with their reduced cell uptake. MUA-NPs display the highest levels of toxicity, caused by autophagy, cell membrane damage, mitochondrial damage, and cytoskeletal deformations. At similar intracellular NP levels, PEG-NPs induce the highest levels of reactive oxygen species (ROS), but do not affect the cell cytoskeleton, in contrast to MUA- and PMA-NPs. Gene expression studies support the findings above, defining autophagy and cell membrane damage-related necrosis as main toxicity pathways. Additionally, immunotoxicity, DNA damage responses, and hypoxia-like toxicity were observed for PMA- and especially MUA-NPs. Together, these data reveal that Ag(+) ions do contribute to Ag NP-associated toxicity, particularly upon intracellular degradation. The different surface properties of the NPs however result in distinct toxicity profiles for the three NPs, indicating clear NP-associated effects.Keywords:
Silver nanoparticle
We report the results of a 28-day oral exposure study in rats, exposed to <20 nm noncoated, or <15 nm PVP-coated silver nanoparticles ([Ag] = 90 mg/kg body weight (bw)), or AgNO(3) ([Ag] = 9 mg/kg bw), or carrier solution only. Dissection was performed at day 29, and after a wash-out period of 1 or 8 weeks. Silver was present in all examined organs with the highest levels in the liver and spleen for all silver treatments. Silver concentrations in the organs were highly correlated to the amount of Ag(+) in the silver nanoparticle suspension, indicating that mainly Ag(+), and to a much lesser extent silver nanoparticles, passed the intestines in the silver nanoparticle exposed rats. In all groups silver was cleared from most organs after 8 weeks postdosing, but remarkably not from the brain and testis. Using single particle inductively coupled plasma mass spectrometry, silver nanoparticles were detected in silver nanoparticle exposed rats, but, remarkably also in AgNO(3) exposed rats, hereby demonstrating the formation of nanoparticles from Ag(+)in vivo that are probably composed of silver salts. Biochemical markers and antibody levels in blood, lymphocyte proliferation and cytokine release, and NK-cell activity did not reveal hepatotoxicity or immunotoxicity of the silver exposure. In conclusion, oral exposure to silver nanoparticles appears to be very similar to exposure to silver salts. However, the consequences of in vivo formation of silver nanoparticles, and of the long retention of silver in brain and testis should be considered in a risk assessment of silver nanoparticles.
Silver nanoparticle
Silver stain
Nanotoxicology
Cite
Citations (673)
In this paper, a cellulose paper was impregnated with silver nanoparticles (AgNPs) for the purpose of removing Enterococcus faecalis from drinking water. AgNPs papers were prepared by chemical reduction of silver nitrate (AgNO3) with various concentrations (0.005 M, 0.01 M, 0.015 M, and 0.025 M) using sodium borohydride (NaBH4) as a reducing agent. Two ratios of NaBH4/AgNO3 of 2:1and 10:1 were used to show the effect of reduction on the formation and removal efficiencies of AgNPs. AgNPs papers were characterized using SEM and TEM. TEM images showed that the silver nanoparticles size in the papers varies from 1.3 to 75 nm.
Silver nanoparticle
Sodium borohydride
Silver nitrate
Enterococcus faecalis
Reducing agent
Chemical reduction
Cite
Citations (1)
The silver (Ag) metal is considered a very useful metal for treating consume wound diseases, open wounds, and cuts, respectively. Nowadays, nanotechnology has created a surprising effect by changing over metallic silver into silver nanoparticles (AgNPs) for better applications. However, according to advanced technology, the synthesis of nanoparticles occurs by using organic or biological techniques rather than physical and chemical techniques. Also, the synthesis of silver nanoparticles (AgNPs) using biological or organic sources is cost-effective and eco-friendly. Silver nanoparticles (AgNPs) are broadly used as antibacterial specialists, helping us cure novel diseases and questionable sicknesses. In biomedicine, silver nanoparticles have huge points of interest because of their physical and synthetic flexibility. The uses of silver nanoparticles (AgNPs) in nano-gels, nano-fluids, silver-based coating over food and medical devices are advancing. Still, there is a need to innovate a better version of silver nanoparticles for vigorous use in an eco-friendly way. So, this review describes the methods of synthesis, activities under various conditions, and different biomedical uses of silver nanoparticles (AgNPs) in detail.
Silver nanoparticle
Environmentally Friendly
Cite
Citations (7)
Recently, the use of biological synthesis of metal nanoparticles has attracted widespread attention. Researchers are trying to find a biological method to synthesize silver nanoparticles with little environmental pollution and easy preparation, and to explore the impact of preparation conditions on the synthesis of silver nanoparticles. This study aims to explore the biological synthesis of silver nanoparticles (AgNPs) with controllable size and good effect and to compare their biological activity with that of AgNPs prepared by chemical method.
Silver nanoparticle
Silver nitrate
Reducing agent
Cite
Citations (0)
Silver nanoparticles (AgNPs) were produced utilizing Marasmius palmivorus MG717877.1 in the current investigation. The fungal cell filtrate was used to accomplish external production of silver nanoparticles (AgNPs) from silver nitrate (AgNo3) solution. The aqueous silver (Ag) ions in a 1mM AgNo3 aqueous solution were decreased when it was exposed to fungal cell filtrate, resulting in highly stable AgNPs. When challenged with 1mM AgNo3 solution, the fungal biomass acquired AgNPs on its surface, inside the cytoplasmic membrane, and within the cytoplasm in 72 hours. UV was used to characterize the AgNPs that had been produced. For M.palmivorus MG717877.1, the greatest absorbance of AgNPs was recorded at 400nm in the visible spectrum. Furthermore, the FTIR and SEM analyses of silver nanoparticles on these fungus were used to characterize silver nanoparticles. In addition, we tested the antifungal activity of M.palmivorus MG717877.1 at various concentrations, including 25, 50, 100, and 150 mg/ml. The findings revealed that silver nanoparticles produced in a concentration-dependent manner have strong antifungal activity on isolates. At 150 mg of AgNPs, the highest decrease was seen for this isolate.
Silver nanoparticle
Silver nitrate
Absorbance
Cite
Citations (0)
Synthesis of silver nanoparticles and the development of silver nanoparticles in analytical method has been done. Silver nanoparticles was prepared by chemical reduction method, with variant of silver nitrate concentration and stirring time to result te best silver nanoparticles. The objective of this research is to determain the optimum condition for synthesis of silver nanoparticles and the ability of silver nanoparticles for a colorimetric sensor. The silver nanoparticles were characterized by UV-Visible and Paricle Size Analyzer (PSA). The result of characterized by UV-visible specthrophotometer showed that the UV-vis absorption spectra of silver nanoparticles with difference silver nitrate concentration gave a difference size of silver nanoparticles and the stirring time controlled the stable colloidal of silver nanoparticles. The size distribution of silver nanoparticles were confirmed by using the Paricle size analyzer (PSA). We have applied colloid silver nanoparticles as colorimetric sensor of Sialic acid and Melamine, the presence of Sialic acid and Melamine induces the aggregation of silver nanoparticles, accompanied by a color change from yellow to red-purplish (Sialic acid) and yellow to brown (melamine)
Silver nanoparticle
Silver nitrate
Cite
Citations (66)
Silver nanoparticles have unique properties which help in molecular diagnostics, in therapies, as well as in devices that are used in several medical procedures. It is known that silver ions and silver based compounds are highly toxic to microorganisms which include 16 major species of bacteria. Silver nanoparticles are particles of silver of size between 1nm to 100 nm. They have gained significant consideration due to their unique characteristics and diverse applications. Fusarium oxysporum, potato dextrose broth and silver nitrate solution can be used for biological synthesis of silver nanoparticles. Colour change of fungal filtrate incubated with silver nitrate to yellowish brown depicted silver nanoparticle formation. These nanoparticles are used for delivery of drugs to their target sites at the right time to have a controlled release and achieve the maximum therapeutic effect.
Silver nanoparticle
Silver nitrate
Cite
Citations (0)
The bactericidal activity of silver nanoparticles is supported by a large number of studies, but their action mechanisms are still a controversial issue due to the role of the silver (I) released from the nanoparticles. In this study, direct analytical methods for detection and identification of dissolved and nanoparticulate silver based on single cell and single particle inductively coupled plasma mass spectrometry (ICP-MS) and hydrodynamic chromatography ICP-MS, in combination with alkaline digestions, have been used. Detection of silver species in Escherichia coli bacteria exposed to silver allowed to confirm the different bactericidal activity associated with silver nanoparticles of different sizes. In the case of 10 nm silver nanoparticles, a combined ion-particle action would be responsible for bactericidal effect, since ionic silver was not detected in the culture medium and both dissolved and particulate silver were detected in the exposed bacteria. On the other hand, bacteria did not internalize 60 nm silver nanoparticles and their bactericidal activity was related to the ionic silver released in the culture medium.
Silver nanoparticle
Particle (ecology)
Cite
Citations (1)
In this paper, a cellulose paper was impregnated with silver nanoparticles (AgNPs) for the purpose of removing Enterococcus faecalis from drinking water. AgNPs papers were prepared by chemical reduction of silver nitrate (AgNO3) with various concentrations (0.005 M, 0.01 M, 0.015 M, and 0.025 M) using sodium borohydride (NaBH4) as a reducing agent. Two ratios of NaBH4/AgNO3 of 2:1and 10:1 were used to show the effect of reduction on the formation and removal efficiencies of AgNPs. AgNPs papers were characterized using SEM and TEM. TEM images showed that the silver nanoparticles size in the papers varies from 1.3 to 75 nm.
Silver nanoparticle
Sodium borohydride
Silver nitrate
Enterococcus faecalis
Reducing agent
Chemical reduction
Cite
Citations (1)
Biologically the silver nanoparticles were synthesized from Indigofera cordifolia leaves extract. The absorbance of the silver nanoparticles centered at four hundred and twenty nm, with respect to the surface plasmon resonance of silver nanoparticles wavelength. XRD method proves, biosynthesized NPs would retain the face centered cubic (fcc) structure. In TEM image analysis, silver NPs morphology was spherical in shape. The composition of the silver nanoparticles was obtained by EDAX analysis method. FTIR analysis concluded that biosynthesis Ag NPs was observed at 1384 cm-1, with respect to –NO3 stretching arises from AgNO3. Ten types of bands are present in the broad emission because of organic matrix bound to silver nanoparticles, which reveals as the result of photoluminescence measurements. The silver NPs possess more antibacterial activity as compared to the standard drug, Amoxicillin.
Silver nanoparticle
Absorbance
Cite
Citations (1)