A Globally Distributed Insecticide Resistance Allele Confers a Fitness Cost in the Absence of Insecticide in Aedes aegypti (Diptera: Culicidae), the Yellow Fever Mosquito
5
Citation
28
Reference
10
Related Paper
Citation Trend
Abstract:
Abstract The cosmopolitan mosquito Aedes aegypti is a vector of harmful arboviruses. Pyrethroid insecticides are used to reduce adult populations and prevent the spread of disease. Pyrethroids target the insect voltage-gated sodium channel (VGSC). Collectively, mutations in Vgsc that confer resistance are referred to as knock-down resistance or kdr. There are numerous kdr mutations found in A. aegypti Vgsc, and there is co-occurrence of some mutations. Full-length cDNA sequences have identified nine known kdr (e.g., 1534C) alleles. The 1534C allele is among the most common kdr alleles, but allele frequencies between populations vary considerably. We used the 1534C:RK strain, which has the 1534C (kdr) allele in the genetic background of the insecticide susceptible Rockefeller (ROCK) strain, and conducted population cage experiments to assess the potential intrinsic fitness cost of the 1534C allele relative to the susceptible allele (F1534) in the ROCK strain. Individuals were genotyped across generations using allele specific PCR. A fitness cost of the 1534C allele was detected across seven generations of mosquitos reared in the absence of insecticide selection pressure. The decrease in allele frequency was not due to drift. Comparison of our results to previous studies suggests that the magnitude of the fitness cost of kdr alleles in the absence of insecticide is disconnected from the level of resistance they confer, and that the fitness costs of different kdr alleles can be variable.Keywords:
Insecticide Resistance
Pesticide resistance
Mosquito control
Abstract The Togolese Republic has a tropical and humid climate which constitutes an ideal environment for mosquitoes to breed and transmit diseases. The Aedes mosquito is known to transmit yellow fever (YF), dengue, chikungunya, and Zika viruses in West Africa. Togo has been suffering from YF virus transmission, despite vaccination efforts. Unfortunately, there is scarcity in the data that reflect mosquito spatial distribution in Togo, specifically possible YF vectors. In the current study, mosquito surveillance efforts targeted areas with confirmed YF cases between July and August 2012. Indoor mosquitoes were collected using knockdown insecticide spraying, whereas Biogents (BG) traps were used to collect outdoor mosquito adults. Mosquito larval surveillance was conducted as well. In total, 17 species were identified. This investigation revealed the presence of medically important vectors in Togo, especially the Aedes aegypti (Linnaeus) (Diptera: Culicidae) which was collected in the four regions. Screening of all pools of female Aedes mosquitoes for YF, by real-time PCR, showed negative results. This is the first record for Coquillettidia flavocincta (Edwards) (Diptera: Culicidae) species in West Africa. This preliminary work serves as a baseline for further mosquito distribution studies in Togo.
Mosquito control
Cite
Citations (4)
This study was aimed to identify the chemical compounds of Aedes aegypti that can be potentially used to develop pheromone-based vector control methods. In this study, we compared the chemical compounds collected from the organs of mosquitoes at different developmental stages in the life cycle. We also compared the composition and amount of extracts from the different tissues of male and female adult mosquito. Interestingly, we found large amount of C17-C20 ethyl and methyl esters in the wings of female and antennae of male mosquito. We also found that isopropyl esters, dodelactone, octadecenoic acid and medium-chain fatty acid increase drastically during the late larval stage (L4). Old adult mosquitoes showed remarkable increase in production of C16:1 and C18:1 methyl esters, as a first example of chemical signatures specifically associated with aging in the animals. This knowledge may open the ground to find new behaviorally-important molecules with the ability to control Aedes specifically.
Cite
Citations (18)
Abstract Aedes aegypti Linnaeus and Aedes albopictus Skuse are vectors of dengue virus and responsible for multiple autochthonous dengue outbreaks in Big Island, Hawai’i. Control of Ae. aegypti and Ae. albopictus has been achieved in In2Care trap trials, which motivated us to investigate this potential control approach in the Big Island. Our In2Care trial was performed in the coastal settlement of Miloli’i in the southwest of Big Island where both Ae. aegypti and Ae. albopictus are found. This trial starting in the second week of July and ending in the last week of October 2019 fell within the traditional wet season in Miloli’i. No significant reduction in egg or adult counts in our treatment areas following 12 wk of two In2Care trap placements per participating household were observed. In fact, an increase in numbers of adults during the trial reached levels that required the local mosquito abatement program to stop the In2Care trap trial and institute a thorough source reduction and treatment campaign. The source reduction campaign revealed a large variety and quantity of water sources competed with the oviposition cups we had placed, which likely lowered the chances of our oviposition cups being visited by pyriproxyfen-contaminated Aedes adults exiting the In2Care traps.
Aedes albopictus
Mosquito control
Pyriproxyfen
Cite
Citations (5)
Cite
Citations (3)
This research is conducted in the University of Gujrat Pakistan. The main objectives of the research were to find best way of mosquito management, to describe different methods of mosquito control, different plants extracts that are used against mosquito and to compare either chemical or botanical control is best. In term of disease transmission and public health importance mosquito are considered as very important group of insects. Population of mosquito’s increases exponentially that is huge problem for many countries because mosquito spread the filarial, encephalitis, malaria, Yellow fever, chikungunya, Japanese encephalitis, dengue, Lyme disease and epidemic poly-arthritis. Different types of mosquito repellents such as synthetic compounds, aromatic oils and herbs are used against mosquitoes. Chemical mosquito repellents have an extraordinary protection profile, but they are noxious as compare to the plant based repellents.
Mosquito control
Culex tritaeniorhynchus
Zika Virus
Cite
Citations (21)
Mosquitoes are the most dangerous vectors of diseases belonging to arthropod family. They are important in public health because of the blood-sucking habits of the females. Mosquitoes were declared as the “public enemy number one” by WHO. Mosquito-borne diseases are increasing due to having dirty places near residential area containing still water, lack of cleanness in public places and decreasing immunity. Mosquitoes are known to transmit some dangerous diseases, including malaria, dengue, yellow fever, filariasis, Zika fever, encephalitis, chikungunya fever, West Nile virus infection in almost all tropical and subtropical countries as well as many other parts of the world. A large number of researchers are evaluating botanical extracts containing active phytochemicals to address some of these problems. From early human ages, various plants that contain toxic chemicals are used for mosquito control. Studies and research on mosquito control are generally aimed at cost reduction and increasing efficacy. This review summarizes research and studies that were conducted previously by researchers on mosquito controlling using botanical pesticides.
Mosquito control
Zika Virus
Cite
Citations (2)
Insecticide Resistance
Cite
Citations (0)
Panama
Cite
Citations (5)
Mosquitoes pose the greatest threat to public health because of their ability to act as vectors of pathogens causing malaria, dengue, yellow fever, encephalitis and filariasis, which affect many millions of people all around the world. Of particular interest, Aedes aegypti is the vector for the arboviruses responsible for yellow fever and dengue fever, present in more than one hundred countries which threatens the health of approximately 2.5 billion people. Worldwide, around 80 million people are infected each year. Substituted α,β-unsaturatedd δ-lactones units are present in a large number of compounds isolated from plants and marine organisms. Representative examples are goniothalamin, and massoialactone (Fig. 1).
Cite
Citations (0)
Under long-term insecticide selection pressure, mosquitoes have evolved in various physiological aspects to become resistant to chemical insecticides, bringing difficulties in the control of diseases transmitted by mosquitoes. In this study we demonstrate the main molecular mechanisms of insecticide resistance and the fitness cost of the relevant resistance genes, and review both Chinese and international strategies for control of mosquito-borne diseases for providing guidance of prevention and control of mosquitoes and vectors in China.
Insecticide Resistance
Mosquito control
Pesticide resistance
Cite
Citations (0)