Partial cholecalciferol replacement with 1,25-dihydroxycholecalciferol glycoside in diets for piglets
Heloíse TrautenmüllerJansller Luiz GenovaLiliana Bury de Azevedo dos SantosIsabela Ferreira LealGleicianny de Brito SantosPaulo Evaristo RupoloRicardo Vianna NunesEduardo Raele de OliveiraPaulo Levi de Oliveira Carvalho
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Context Vitamin D supplementation plays a key role because its actions positively affect the animal’s overall health for optimal performance. Aims To assess partial cholecalciferol replacement with 1,25-dihydroxycholecalciferol glycoside for piglets on nutrient digestibility and daily balance of calcium and phosphorus, growth performance and blood metabolites. Methods To test digestibility, a total of 36 whole male piglets (18.79 ± 3.37 kg BW) were assigned in a randomised complete block design, with four treatments: (1) D3 (100% of the vitamin D supplemented with 1969 IU of cholecalciferol), (2) no supplemental sources of vitamin D (control), (3) D3 (50% of requirement + 0.375 μg of 1,25(OH)2D3 glycoside) or (4) 100% supplemented with 0.750 μg of 1,25(OH)2D3 glycoside. Nine replicates were performed, with one animal per experimental unit. For growth performance (Experiment II), a total of 128 whole male piglets (6.82 ± 0.38 kg BW) were distributed in a randomised complete block design, with four treatments: (1) 100% D3 (2707 IU in the pre-starter phase I, 2405 IU in the pre-starter phase II and 1969 IU in the starter phase), (2) 50% D3 + 0.25 μg of 1,25(OH)2D3 glycoside, (3) 25% D3 + 0.375 μg of 1,25(OH)2D3 glycoside or (4) 100% supplemented with 0.50 μg of 1,25(OH)2D3 glycoside. Eight replicates were conducted, with and four animals per experimental unit. Key results The apparent digestibility of nutrients and mineral balance were not influenced (P > 0.1). The results of Experiment II indicate effects (P < 0.1) of vitamin D supplementation on the growth performance evaluated during the nursery phase. Plasma calcium concentrations in the pre-starter II phase showed (P < 0.1) the highest concentration in the 50/50 treatment. Alkaline phosphatase showed (P < 0.001) a difference between treatments in the starter phase, with treatment 25/75 promoting the lowest plasma value. Conclusions Cholecalciferol or 1,25-dihydroxycholecalciferol glycoside resulted in similar digestibility and balance of calcium and phosphorus, even though the combination increased plasma calcium and alkaline phosphatase concentration in piglets. In addition, the partial replacement reduced the voluntary feed intake of piglets during nursery phase. Implications This investigation provided new information on partial cholecalciferol replacement with 1,25-dihydroxycholecalciferol glycoside in piglet starter as an alternative in post-weaning nutrition.Keywords:
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Context Vitamin D supplementation plays a key role because its actions positively affect the animal’s overall health for optimal performance. Aims To assess partial cholecalciferol replacement with 1,25-dihydroxycholecalciferol glycoside for piglets on nutrient digestibility and daily balance of calcium and phosphorus, growth performance and blood metabolites. Methods To test digestibility, a total of 36 whole male piglets (18.79 ± 3.37 kg BW) were assigned in a randomised complete block design, with four treatments: (1) D3 (100% of the vitamin D supplemented with 1969 IU of cholecalciferol), (2) no supplemental sources of vitamin D (control), (3) D3 (50% of requirement + 0.375 μg of 1,25(OH)2D3 glycoside) or (4) 100% supplemented with 0.750 μg of 1,25(OH)2D3 glycoside. Nine replicates were performed, with one animal per experimental unit. For growth performance (Experiment II), a total of 128 whole male piglets (6.82 ± 0.38 kg BW) were distributed in a randomised complete block design, with four treatments: (1) 100% D3 (2707 IU in the pre-starter phase I, 2405 IU in the pre-starter phase II and 1969 IU in the starter phase), (2) 50% D3 + 0.25 μg of 1,25(OH)2D3 glycoside, (3) 25% D3 + 0.375 μg of 1,25(OH)2D3 glycoside or (4) 100% supplemented with 0.50 μg of 1,25(OH)2D3 glycoside. Eight replicates were conducted, with and four animals per experimental unit. Key results The apparent digestibility of nutrients and mineral balance were not influenced (P > 0.1). The results of Experiment II indicate effects (P < 0.1) of vitamin D supplementation on the growth performance evaluated during the nursery phase. Plasma calcium concentrations in the pre-starter II phase showed (P < 0.1) the highest concentration in the 50/50 treatment. Alkaline phosphatase showed (P < 0.001) a difference between treatments in the starter phase, with treatment 25/75 promoting the lowest plasma value. Conclusions Cholecalciferol or 1,25-dihydroxycholecalciferol glycoside resulted in similar digestibility and balance of calcium and phosphorus, even though the combination increased plasma calcium and alkaline phosphatase concentration in piglets. In addition, the partial replacement reduced the voluntary feed intake of piglets during nursery phase. Implications This investigation provided new information on partial cholecalciferol replacement with 1,25-dihydroxycholecalciferol glycoside in piglet starter as an alternative in post-weaning nutrition.
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The perfused small intestine from a vitamin D deficient rat exhibits about one-half the calcium transport of the intestine from a rat given vitamin D. These levels of calcium transport can be maintained for at least 4 hours. Addition of 2.5 micrograms of 25-hydroxycholecalciferol added to the vitamin D deficient intestine via the arterial blood perfusate induces a rise in calcium transport to +D levels within 2 hours. In contrast, 250 micrograms of vitamin D3 given in the same manner has no effect on the calcium transport level over a 4-hour period. These data provide strong evidence that 25-hydroxycholecalciferol represents the metabolically active form of vitamin D3.
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Regulation of prepro-PTH and vitamin D receptor (VDR) mRNAs in the parathyroid glands was studied in chickens in vivo. The birds were raised to 21 days of age on a vitamin D-deficient diet with 1% calcium and 0.65% phosphorous. At the end of this period, the chicks exhibited marked hypocalcemia and enlarged parathyroid glands. In three separate trials, the birds were repleted for 6 days with vitamin D and different dietary calcium and phosphate concentrations, with 2 micrograms/kg 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and different dietary calcium concentrations (0.5%, 1.0%, or 1.8%), or with 2 or 10 micrograms/kg 1,25-(OH)2D3 and 0.6% or 1.9% calcium or were kept vitamin D3 deficient and fed 0.5%, 1.0%, or 1.8% dietary calcium. Vitamin D treatment when combined with a high level of dietary calcium resulted in an increase in plasma calcium from 6 mg/dl to greater than 10 mg/dl, a decrease in PTH mRNA of 65%, and a 6- to 8-fold increase in VDR mRNA. In another experiment in which no vitamin D source was given and the diets contained increasing levels of dietary calcium, plasma calcium increased significantly (5.5 vs. 7 mg/dl), while PTH mRNA decreased by 40% and VDR mRNA increased by 60%. Neither parathyroid gland weight nor total RNA was significantly affected. When chicks were repleted with 1,25-(OH)2D3, the increase in plasma calcium and VDR mRNA and the decrease in PTH mRNA were considerably more pronounced than those in the absence of the vitamin D source. Furthermore, in the presence of the hormone, parathyroid weight and total RNA decreased significantly with increasing concentrations of dietary calcium. When the chicks were repleted, respectively, with the two levels of 1,25-(OH)2D3, a marked positive interaction was evident between the hormone and dietary calcium in affecting levels of PTH and VDR mRNA. These results suggest that both 1,25-(OH)2D3 and calcium participate in the regulation of PTH and VDR gene transcription in the avian parathyroid gland. Whereas the action of 1,25-(OH)2D3 requires a minimal level of dietary calcium, calcium affects PTH and VDR gene transcription even in the absence of any vitamin D source.
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Calcium ions were infused into the external jugular vein of 2 groups of rachitic chicks. One group had been injected with 5 micrograms cholecalciferol (vitamin D3), while the other group remained vitamin D-deficient. After approximately 72 h the chickens were killed and intestinal mucosa homogenates were prepared for the measurement of calcium-binding protein (CaBP). The results indicate that CaBP is not biosynthesised in response to raising the extracellular calcium ion concentration, and only appears if the chickens had received cholecalciferol.
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The effect of dietary vitamin D levels on the response to iv injected parathyroid hormone (PTH) was studied in chicks fed one of three diets: D-deficient, Control-D (1.4 IU cholecalciferol/g diet), or High-D (70 IU cholecalciferol/g diet) during the first 4 weeks post-hatching. Compared to chicks on Control-D diet, chicks on the D-deficient diet had significantly decreased plasma Ca levels at 2 and 4 weeks and increased plasma P levels at 17 and 21 days. The plasma Ca response to a low dose of PTH (15 USP U/100 g body wt) 1 hr postinjection was normal at 1 week, reduced at 2 weeks and absent at 4 weeks in Ddeficient chicks. However, a 4–16-fold higher dose of PTH did elicit a significant, though subnormal, response in this group at 3 and 4 weeks. Chicks fed the D-deficient diet with 2.8% Ca, compared to 1.4% Ca, showed a near normal plasma Ca level and bone ash content and only a small increase in plasma P at 17 and 21 days. However, the plasma Ca response to 15 U PTH/100 g body wt in this group was significantly increased only at 17 days and not at 21 days. In contrast, the hyperphosphatemic response to PTH was not markedly diminished in the D-deficient group, and it was restored to Control-D levels in the D-deficient High-Ca group. These data suggest that different mechanisms may be involved in the Ca and P responses. (Endocrinology96: 275, 1975)
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1. Eighteen diets supplying all combinations of three phosphorus contents (3.1, 4.0 and 4.8 g non‐phytate P/kg) and six vitamin D supplements (37.5 or 150 μg cholecalciferol/kg; or 16 or 24 μg 25‐hydroxy‐cholecalciferol/kg; or 37.5 μg cholecalciferol/kg with either 16 or 24 μg 25‐hydroxycholecalciferol/kg) were fed to 2 880 pullets of two stocks from 64 to 74 weeks of age. The birds were housed in eight light‐proof rooms, four of which had 24‐h light‐dark cycles (16L : 8D) and four had 28‐h cycles (20L : 8D). 2. The dietary treatments had no significant effect upon food intake, egg output, shell thickness, shell deformation or specific gravity of the eggs. 3. The 28‐h cycle reduced mean rate of lay by 4.5%, increased egg weight by 5.8% and increased shell thickness by 9.4%. The proportion of eggs with shell faults revealed on candling was reduced from 4.1 % to 2.8%. 4. It is concluded from this and other sources that decreasing dietary phosphorus or modifying vitamin D supplements may sometimes lead to increases in shell thickness of the order of 1 to 2%, but that these changes are unlikely to result in a measurable reduction in the proportion of cracked eggs late in the laying year. 5. A 28‐h light‐dark cycle results in a longer and more uniform interval between consecutive ovipositions and thus gives reliable increases in shell thickness which are large enough to reduce the proportion of cracked eggs in many practical situations. Whether it is profitable to use an ahemeral cycle will depend upon the relative prices paid for eggs of different sizes.
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The experiment was carried out on 720 Cobb 500 broiler chickens, reared to 42 days. Various forms of vitamin D3 were the group-differentiating factors (cholecalciferol and calcidiol), given in starter, grower and finisher feed mixtures. The control group (group I) received the feed mixture which contained 4000 IU of vitamin D3 (cholecalciferol), group II received 2500 IU of vitamin D3 and 1500 IU of calcidiol (25-OH-D3) and group III – 1240 IU of vitamin D 3 and 2760 IU of calcidiol (25-OH-D 3 ). The aim of the study was to determine the effect of different forms of vitamin D 3 (cholecalciferol and calcidiol) in feed on production yield and meat quality. The results showed the usefulness of partial replacement of vitamin D3 with calcidiol. With the application of 1500 IU (25-OH-D3) in the diet of broiler chickens, higher body weight at the end of the rearing period was obtained in comparison to the birds from the control group. The chickens from group III, receiving 2760 IU of calcidiol in their diet, had lower body weight on the 42 nd day in comparison to the chickens from group II; but also a lower mortality and the lowest feed conversion per one kg of body weight gain was observed in that group. The partial replacement of vitamin D 3 with calcidiol in chickens’ nutrition did not have any effect on dressing percentage of males and females; differences were only found in the percentage of offals, especially in the increased heart mass in males from group III. Improvement was observed in the physicochemical properties (higher water absorption and lower drip after thermal treatment) and in the chemical composition of meat in the group of chickens fed the diet with the addition of calcidiol. Leg muscles from the chickens from group II had higher protein content. Fat in leg muscles and abdominal fat of the chickens from the experimental groups (group II and III) included a significantly higher quantity of monounsaturated fatty acids and a lower content of polyunsaturated acids, especially from the n-6 family, as compared to the control group. Moreover, the addition of calcidiol in the mixtures caused a decrease in the rate of oxidation of lipids in abdominal fat of the chickens in comparison to the control group.
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Regulation of prepro-PTH and vitamin D receptor (VDR) mRNAs in the parathyroid glands was studied in chickens in vivo. The birds were raised to 21 days of age on a vitamin D-deficient diet with 1% calcium and 0.65% phosphorous. At the end of this period, the chicks exhibited marked hypocalcemia and enlarged parathyroid glands. In three separate trials, the birds were repleted for 6 days with vitamin D and different dietary calcium and phosphate concentrations, with 2 micrograms/kg 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and different dietary calcium concentrations (0.5%, 1.0%, or 1.8%), or with 2 or 10 micrograms/kg 1,25-(OH)2D3 and 0.6% or 1.9% calcium or were kept vitamin D3 deficient and fed 0.5%, 1.0%, or 1.8% dietary calcium. Vitamin D treatment when combined with a high level of dietary calcium resulted in an increase in plasma calcium from 6 mg/dl to greater than 10 mg/dl, a decrease in PTH mRNA of 65%, and a 6- to 8-fold increase in VDR mRNA. In another experiment in which no vitamin D source was given and the diets contained increasing levels of dietary calcium, plasma calcium increased significantly (5.5 vs. 7 mg/dl), while PTH mRNA decreased by 40% and VDR mRNA increased by 60%. Neither parathyroid gland weight nor total RNA was significantly affected. When chicks were repleted with 1,25-(OH)2D3, the increase in plasma calcium and VDR mRNA and the decrease in PTH mRNA were considerably more pronounced than those in the absence of the vitamin D source. Furthermore, in the presence of the hormone, parathyroid weight and total RNA decreased significantly with increasing concentrations of dietary calcium. When the chicks were repleted, respectively, with the two levels of 1,25-(OH)2D3, a marked positive interaction was evident between the hormone and dietary calcium in affecting levels of PTH and VDR mRNA. These results suggest that both 1,25-(OH)2D3 and calcium participate in the regulation of PTH and VDR gene transcription in the avian parathyroid gland. Whereas the action of 1,25-(OH)2D3 requires a minimal level of dietary calcium, calcium affects PTH and VDR gene transcription even in the absence of any vitamin D source.
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