True Spore Germination Inhibitor of a Cellular Slime MoldDictyostelium discoideum
2
Citation
0
Reference
10
Related Paper
Abstract:
Journal Article True Spore Germination Inhibitor of a Cellular Slime Mold Dictyostelium discoideum Get access Yoshimasa Tanaka, Yoshimasa Tanaka Genetics Laboratory, Isotope Research Institute, Fukazawa, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Kaichiro Yanagisawa, Kaichiro Yanagisawa Genetics Laboratory, Isotope Research Institute, Fukazawa, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Yoichi Hashimoto, Yoichi Hashimoto Genetics Laboratory, Isotope Research Institute, Fukazawa, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Masashi Yamaguchi Masashi Yamaguchi Department of Biology, Tokyo Metropolitan University, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 38, Issue 3, 1 March 1974, Pages 689–690, https://doi.org/10.1080/00021369.1974.10861219 Published: 01 March 1974 Article history Received: 27 December 1973 Published: 01 March 1974Keywords:
Slime Mold
Biological sciences
Abstract Uridine diphosphate glucose pyrophosphorylase (EC 2.7.7.9) from the cellular slime mold, Dictyostelium discoideum, has been characterized. The specific enzyme activity increases 10-fold during the course of morphogenesis. This increase is sensitive to coincident inhibition of protein synthesis by actidione and prior inhibition of ribonucleic acid synthesis by actinomycin D. A new method of assay was developed which can be applied generally to nucleoside diphosphate-sugar pyrophosphorylase reactions.
Slime Mold
Mycetozoa
Cite
Citations (82)
Journal Article True Spore Germination Inhibitor of a Cellular Slime Mold Dictyostelium discoideum Get access Yoshimasa Tanaka, Yoshimasa Tanaka Genetics Laboratory, Isotope Research Institute, Fukazawa, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Kaichiro Yanagisawa, Kaichiro Yanagisawa Genetics Laboratory, Isotope Research Institute, Fukazawa, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Yoichi Hashimoto, Yoichi Hashimoto Genetics Laboratory, Isotope Research Institute, Fukazawa, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Masashi Yamaguchi Masashi Yamaguchi Department of Biology, Tokyo Metropolitan University, Setagaya, Tokyo 158, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 38, Issue 3, 1 March 1974, Pages 689–690, https://doi.org/10.1080/00021369.1974.10861219 Published: 01 March 1974 Article history Received: 27 December 1973 Published: 01 March 1974
Slime Mold
Biological sciences
Cite
Citations (11)
Slime Mold
Mycetozoa
Cite
Citations (27)
Slime Mold
Cellular metabolism
Mycetozoa
Cite
Citations (21)
The cellular slime mold Dictyostelium dticoideum has been extensively studied (1) because of the unique series of morphological transitions that the wild type undergoes.From single, vegetative cells, the organisms pass through complex aggregation, migration, and culmination, leadii finally to spore formation.Sussmenn (2) has produced a number of stable, aggregateless mutants that remain permanently in the vegetative stage as single ameboid cells.'One of these mutants was used in the present study in order to avoid the complicating factors of morphological transition.Previous investigation of the lipids of Dictyostelium diecoideum and its aggregateless mutant Agg 204 had been limited to the characterisation of a steroid, AZ-stigmasten3&ol, and the demonstration of its biosynthesis from acetate and mevalonate (3).Stigmastenol is produced in large quantities by Dictyostelium disco&&urn and is felt to be related to the activity of the aggregation-inducing factor, acrasin (4).The fatty acids and phosphatides of the cellular slime mold are described in this paper.The fatty acid composition of each of the phosphatides has been determined.Evidence is presented for considerable molecular heterogeneity within several classes of phosphatides.The phosphatides contain an unusually high percentage of unsaturated fatty acids.Of major interest is the presence, in large amounts, of three new dienoic long chain fatty acids whose structures have been established as 5,9-hexadecadienoic acid, 5,9-octadecadienoic acid, and 5,1 l-octadecadienoic acid.Under appropriate conditions of growth, two other new fatty acids, 9-heptadecenoic acid and 5,9-heptadecadienoic acid, normally present in small amounts, can be made to accumulate.Studies of the pathways of biosynthesis of these fatty acids are presented in an accompanying paper (5). EXPERIMENTAL PBOCEDUBEGrozvth of Cells-An aggregateless mutant of Dictyostelium disco&urn,4 Agg 204, was maintained as a stock culture by clonal passage at least once every 2 weeks on SM agar (6), with a relatively penicillin-sensitive strain of Escherichia coli B/r as the substrate organism.After full growth of the amebae was permitted at room temperature, cultures were stored at 2" until used.
Slime Mold
Mycetozoa
Cite
Citations (99)
Slime Mold
Mycetozoa
Cite
Citations (23)
Eight hours after the onset of morphogenesis, an inhibitor of transfer ribonucleic acid methylases appears in differentiating Dictyostelium discoideum . The inhibitor is also present in spores. Fifty per cent of the inhibiting activity is lost upon heating at 100 C for 5 min; it is nondialyzable and sensitive to trypsin.
Slime Mold
Mycetozoa
Cite
Citations (22)
Slime Mold
Mycetozoa
Cite
Citations (1)
Slime Mold
Mycetozoa
Cite
Citations (2)
The cellular slime mold Dictyostelium discoideum grows as unicellular free-living amoebae in the presence of nutrients. Upon starvation, the amoebae aggregate and form multicellular structures that each consist of a stalk and spores. D. discoideum encodes at least four proteins (Sir2A, Sir2B, Sir2C, and Sir2D) homologous to human SIRT. RT-PCR and WISH analyses showed that the genes for Sir2A, Sir2C, and Sir2D were expressed at high levels in growing cells but at decreased levels in developing cells, whereas the gene encoding Sir2B was expressed in the prestalk-cell region in the developmental phase.
Slime Mold
Multicellular organism
Mycetozoa
Cite
Citations (2)