Analysis of the molecular mass heterogeneity of the transferrin receptor in Neisseria meningitidis and commensal Neisseria
17
Citation
0
Reference
10
Related Paper
Citation Trend
Keywords:
Neisseria
Neisseriaceae
Transferrin receptor
Commensalism
Identicult-Neisseria (Scott Laboratories, Inc., Fiskeville, R.I.), a rapid enzymatic method with chromogenic substrates, was tested in our laboratories for the identification of Neisseria gonorrhoea, Neisseria meningitidis, and Neisseria lactamica. The test correlated very highly in its identification of pathogenic Neisseria spp. with modified New York City fermentation medium. Identicult-Neisseria appeared to be more sensitive in its detection of prolylaminopeptidase activity in N. meningitidis than most of the currently available systems.
Neisseria
Neisseria gonorrhoeae
Neisseriaceae
Cite
Citations (11)
During 1971 and 1972, 71 cultures of neisseriae that attacked lactose were received by this laboratory. All strains except one from an eye swab were from the nasopharynx of healthy subjects. Nineteen similar strains from the nasopharynx were isolated in this laboratory. The characteristics of these strains were compared with those of Neisseria meningitidis, Neisseria pharyngis, Neisseria catarrhalis, and Neisseria lactamicus. The 90 strains under investigation closely resembled Neisseria meningitidis but could be differentiated by production of acid from lactose and beta-galactosidase activity and were therefore classified as Neisseria lactamicus.
Isolation
Neisseria
Cite
Citations (5)
The purpose of this review is to present the currently available information on genetic loci that have been identi- fied in Neisseria gonorrhoeae and Neisseria meningitidis.We will include genes that were identified by mutations that alter the phenotype of the organism, if the gene was trans- ferred into another genetic background, and genes whose products were well characterized, even if the genes were not identified by mutation.We also will include genes that were cloned and identified either by complementation of Esche- richia coli mutations or by identification of the gene product. GENETIC NOMENCLATUREWe will attempt to establish genetic nomenclatures for these genes, following the guidelines of Demerec et al. ( 29), i.e., assigning a three-letter lowercase designation for the gene family, followed by a capital letter, as appropriate.Genes that have been identified by complementation of well-characterized E. coli mutants will be given the corre- sponding genetic symbol, if appropriate.This is not meant to imply that the neisserial gene encodes the same enzyme as the E. coli gene, but, rather, that it encodes an enzyme that performs the same function in converting the substrate into the product.For cloned neisserial genes that were identified by the gene products, which are not found in E. coli, we will use the previously published nomenclatures, except for genes whose names have been changed by agreement of researchers attending the Sixth International Pathogenic Neisseria Meeting, October 1988.Genes that were identified and named in N. gonorrhoeae should be given the same gene symbol if they are subsequently found to occur in N. meningitidis, and vice versa.Since N. gonorrhoeae and N. meningitidis contain several copies of closely related genes, a special nomenclature has been agreed upon to identify these genes.This consists of giving the genetic symbol following by a subscript notation that identifies the strain containing the gene with a designa- tion of the allele number.For example, the protein lIb gene from N. gonorrhoeae FA1090 would be opaFA1090-2.Genetic designations for these multicopy genes, e.g., as opaA, are not to be given until the gonococcal chromosome has been genetically or physically mapped and the location of the gene on the map has been determined.New genetic symbols or proposed revisions of old nomen- clature are to be approved by Virginia L. Clark, Department of Microbiology and Immunology, Box 672, School of Med- icine and Dentistry, University of Rochester,
Neisseria gonorrhoeae
Neisseriaceae
Neisseria
Linkage (software)
Genetic linkage
Cite
Citations (5)
Neisseria
Neisseria gonorrhoeae
Medical microbiology
Neisseriaceae
Identification
Cite
Citations (18)
Neisseria gonorrhoeae
Neisseriaceae
Moraxella (Branhamella) catarrhalis
Neisseria
Cite
Citations (0)
Neisseria
Neisseria gonorrhoeae
Neisseriaceae
Cite
Citations (55)
Penicillin-resistant (penr) clinical isolates of Neisseria meningitidis, which do not produce beta-lactamase, were first identified in Spain in 1985; the frequency of their recovery, which has been increasing in the past few years, reached 20% in 1989. Serogrouping, determination of serotypes and subtypes, and multilocus enzyme electrophoresis of the penr strains showed an extensive diversity. Resistance is due, at least in part, to a decreased affinity of penicillin-binding protein (PBP) 2 for penicillin. Similar low-affinity forms of PBP 2 are also found in penr isolates of Neisseria lactamica, Neisseria polysaccharea, and Neisseria gonorrhoeae. Genetic transformation of an N. meningitidis type strain to low-level penicillin resistance with DNA from resistant meningococci and other Neisseria species resulted in transformants that possessed low-affinity forms of PBP 2. These altered forms of PBP 2 have been shown to arise from recombinational events that replace parts of the PBP 2 gene with the corresponding regions from the PBP 2 genes of commensal Neisseria species.
Neisseria
Neisseriaceae
Neisseria gonorrhoeae
Penicillin binding proteins
Molecular Epidemiology
Cite
Citations (144)
A rapid enzymatic method using chromogenic substrates for the rapid identification of pathogenic neisseria (Identicult‐Neisseria, Scott Laboratories Inc., CA, USA) was tested in parallel with the rapid carbohydrate utilization test (RCUT) and the Phadebact Monoclonal GC Test against 198 consecutive clinical isolates of oxidase‐positive Gram‐negative diplococci (118 Neisseria gonorrhoeae, 76 N. meningitidis and four N. lactamica). On initial testing the Identicult‐Neisseria gave a 95% overall concordance (97.5%N. gonorrhoeae, 90.8%N. meningitidis). with the RCUT and Phadebact tests; the corresponding figures after repeat testing were 98% overall concordance (98.3%N. gonorrhoeae, 97.4%N. meningitidis). Two of the three strains of N. gonorrhoeae mis‐identified as N. meningitidis on primary testing were also mis‐identified on repeat testing. Seven strains of N. meningitidis were mis‐identified on initial testing (six as Moraxella catarrhalis and one as N. lactamica) and two on repeat testing (both as Mor. catarrhalis). We conclude that the Identicult‐Neisseria is not sufficiently reliable for the culture confirmation of gonococci and meningococci.
Neisseria
Identification
Neisseria gonorrhoeae
Cite
Citations (0)
Neisseriaceae
Neisseria
Neisseria gonorrhoeae
Cite
Citations (0)
Neisseria
Neisseriaceae
Transferrin receptor
Commensalism
Cite
Citations (17)