Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract
Xiaoyu ZhaoChenpei ZhaoLeining YangLinlin JiangJianlong ZhangXin YuGuozhong ChenHongwei ZhuWenli TangYouzhi LiMaolian WeiXingxiao ZhangHong Peng Jia
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Abstract:
The beneficial effects of the probiotic strain Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) are based on its adherence and colonization ability in the gut. However, little is known about the migration and long-term gut colonization of the strain. This study evaluated the gut colonization modes of Lactiplantibacillus plantarum RS-09 to identify the strain with long-term gut colonization potential. We established CFDA/SE-labeled RS-09 to study the temporal and spatial distribution of RS-09 in the intestine as well as to analyze its persistence in different parts of the intestine by flow cytometry. This study has shown that the RS-09 strain maintains strong adhesion abilities under acid (pH 2.5) and base (pH 8.5) conditions. In addition, CFDA/SE can be used as an indicator for the labeling of L. plantarum RS-09 in the intestinal tract in vivo. We established a growth kinetics model of RS-09 to elucidate its persistence in the intestine. In vivo persistence experiments showed that the persistence rate of RS-09 was the highest in the cecum (69.5%) and the lowest in the duodenum (12.8%) at 96 h. After 20 days, RS-09 was predominantly localized in the cecum and colon steadily. These studies provide new insights into the long-term persistence of L. plantarum in the gastrointestinal tract. The CFDA/SE label system may be used to study the in vivo colonization dynamics of other probiotic strains.Keywords:
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Lactobacillus plantarum strain ZJ316, a probiotic strain with several functions, was isolated from healthy newborn infant fecal samples. Here we report the finished and annotated genome sequence of this organism.
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In scrapings of mouse cecal mucosae, motile bacteria outnumbered nonmotile bacteria by a ratio of 2:1. Obligately anaerobic bacteria were obtained from such scrapings through the use of techniques designed for the selective isolation of motile bacteria. One of the isolates, Roseburia cecicola, was rapidly motile in broth by means of 20 to 35 flagella arranged in a fascicle on each cell. R. cecicola cells colonized germfree mice (3 x 10(9) to 1 x 10(10) CFU/g of cecum) within 11 days after the animals were inoculated intragastrically with 2 x 10(8) CFU per mouse. In such monoassociated gnotobiotes, the bacteria were found primarily in the cecum, dispersed in the lumen among particles of digesta, and in the mucus over the epithelial surface. Between 2 and 3 weeks after birth, offspring of monoassociated adult mice were colonized by the bacterium (2 x 10(9) to 1 x 10(10) CFU/g of cecum). These results indicate that R. cecicola is suitable for studies of the ecology of host-associated microorganisms, particularly for investigation of the role of motility and possibly also chemotaxis in bacterial colonization of the mammalian gastrointestinal tract.
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The expressions of CCL28 in the gastrointestinal tract of 15-day-old and 30-day-old piglets were determined by using RT-PCR method.The results showed that CCL28 mRNA was expressed in duodenum,ileum,cecum,colon,rectum of 15-day-old piglet and in duodenum,jejunum,ileum,cecum,colon,rectum and stomach tissues of 30-day-old piglet;no expression in duodenum,jejunum and stomach tissues,a little in ileum,colon tissues of 15-day-old piglet and in duodenum,jejunum,cecum,stomach tissues of 30-day-old piglet.The time-space expression of CCL28 showed that it was related to the gastrointestinal mucosal immune system development in the piglets and roles in different tissues.
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SUMMARY A coagulase-positive strain of Staphylococcus aureus grown in vitro (strain O) was passaged in rabbits to increase virulence. The passaged organisms grown in vitro (strain P) were used to infect rabbits from which organisms grown in vivo (strain V) were isolated. By LD50 measurement in rabbits, strain V was 25 times more virulent than strain P and 6000 times more virulent than strain O. On incubation in rabbit serum, strain V multiplied whilst strains P and O decreased in viable number (strain O the more rapidly) during the first 3 hr, recovering subsequently. In lysates of polymorphonuclear leucocytes, strain V multiplied or was slightly reduced in number; strains P and O decreased in number (strain O the more rapidly). The findings suggest that resistance to serum and polymorph bactericidins is an important attribute of the virulence of staphylococci and that the substances responsible are qualitatively different or quantitatively increased on growth in vivo.
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Objective To investigate on the probiotic properties of Photosynthetic bacteria strain 2c. Method The resistance ability of strain 2c to acid and ox bile salt along with the property of adhesion to HT-29 cell and inhibiting pathogenic bacteria in vitro were assessed using routine methods. The survival and persistance time in vivo after intake by rat were also studied with bacteria culture.Result 30 min treatment with pH 2.5 and 0.5% or 0.9% ox bile salt had no significant effect on the survival of strain 2c(P0.05).The strain 2c had no inhibiting ability to 4 clinical common pathogenic bacteria. The adhesion ability of strain 2c to HT-29 cell was common. The survival and standing time of 2c in vivo after intaken by rat was relative to intake content.The strain 2c couldn't be enriched 3 days after intaken. Conclusion The strain 2c has preferable probiotic properties.
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Lactobacillus plantarum strain FMNP01 is a new strain with probiotic properties that was isolated from fresh mango from Guangzhou, China. Here, we report the complete genome of this organism.
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Expression of five zinc transporters (ZnT1, 4, 5, 6, and 7) of the Slc30 family in the mouse gastrointestinal tract was studied by immunohistochemical analysis. Results demonstrated unique expression patterns, levels, and cellular localization among ZnT proteins in the mouse gastrointestinal tract with some overlapping. ZnT1 was abundantly expressed in the epithelium of the esophagus, duodenum of the small intestine, and cecum of the large intestine. ZnT4 was predominantly detected in the large intestine. ZnT5 was mainly expressed in the parietal cell of the stomach and in the absorptive epithelium of the duodenum and jejunum. ZnT6 was predominantly detected in the chief cell of the stomach, columnar epithelial cells of the jejunum, cecum, colon, and rectum. Lastly, ZnT7 was observed in all epithelia of the mouse gastrointestinal tract with the highest expression in the small intestine. Expression of ZnT proteins in the absorptive epithelial cell of the gastrointestinal tract suggests that ZnT proteins may play important roles in zinc absorption and endogenous zinc secretion.
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Abstract Background Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum , isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae . Strains were evaluated for their physiological, safety, and antimicrobial characteristics. Results The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae . All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. Conclusion The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.
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