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    Expression of CCL28 in Gastrointestinal Tract of Piglets
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    Abstract:
    The expressions of CCL28 in the gastrointestinal tract of 15-day-old and 30-day-old piglets were determined by using RT-PCR method.The results showed that CCL28 mRNA was expressed in duodenum,ileum,cecum,colon,rectum of 15-day-old piglet and in duodenum,jejunum,ileum,cecum,colon,rectum and stomach tissues of 30-day-old piglet;no expression in duodenum,jejunum and stomach tissues,a little in ileum,colon tissues of 15-day-old piglet and in duodenum,jejunum,cecum,stomach tissues of 30-day-old piglet.The time-space expression of CCL28 showed that it was related to the gastrointestinal mucosal immune system development in the piglets and roles in different tissues.
    Keywords:
    Cecum
    Jejunum
    Large intestine
    In attempting to follow the activity of certain food poisoning organisms in the intestine it was found that before such an investigation could be made more must be learned regarding the in vivo activity of bacteria normally inhabiting the intestine. The enormous number of bacteria of different types usually present in the ileum and colon obscure the pathogenic significance of any one organism. The majority of previous studies have been made on feces because of the technical difficulties encountered in obtaining specimens from various levels of the intestine under physiologic conditions. We have attempted to use a different technic by maintaining normal physiologic conditions in dogs and in monkeys by means of fistulas into different levels of the intestine in some experiments and by isolating segments of the small and large intestine in others. Particular attention was given to the relationship and maintenance of certain bacteria in the intestine and the data are presented under the following headings : (1) technic, (2) the fate of Bacterium coli and Bacterium prodigiosum when introduced in food and injected into the stomach of monkeys, (3) bacterial activity in the small intestine and (4) bacterial activity in the colon.
    Citations (50)
    1. The mucosal and serosal surface dimensions of the rat duodenum, jejunum and ileum were obtained by measurement of photographs of histological sections. The surface areas were calculated from these data. 2. Measurements were made on the intestines from virgin rats and from rats on the sixteenth day of lactation. The mucosal area/unit length of serosa was appreciably increased in all parts of the intestine during lactation. The mucosal area/serosal area (MA/SA) ratio was also markedly increased, but to a lesser extent distally, where an important component of the intestinal hypertrophy was dilatation of the intestine.
    Jejunum
    Intestinal mucosa
    Herein has been presented the results secured in determining the pH of the gastrointestinal tract of the hen, the rabbit and the cat by means of the quinhydrone electrode.
    Fowl
    Rabbit (cipher)
    Hydrogen ion
    Citations (11)
    Background. Ileo-jejunal transposition (IJT; transposition of the distal quarter of the small intestine into the proximal jejunum) is known to stimulate mucosal growth of the transposed ileum, but the effects on other parts of the small intestine are controversial. The effect of aging on the trophic action of IJT is not known. Methods. We examined the trophic effect of IJT (3 weeks post-operation) on the gastrointestinal tract and pancreas, and on plasma levels of neurotensin and gastrin in three different aged groups of Fischer 344 rats (4, 12, and 24 months old). Results. Three weeks after IJT, the mucosal mass, villus height, and crypt depth increased significantly in the transposed ileum as well as in the remainder of the small intestine. The weights of the colon and pancreas increased significantly after IJT. These responses were not affected by aging. In each of the three age groups, IJT did not affect plasma gastrin level, but significantly increased plasma level of neurotensin. Conclusions. The distal ileum appears to play an important role in the regulation of growth in the intestine and pancreas; this role is preserved in aged rats. Neurotensin may play an important role in this mechanism.
    Jejunum
    Transposition (logic)
    Citations (12)