Roles of a Cysteine Desulfhydrase LCD1 in Regulating Leaf Senescence in Tomato
Kang‐Di HuXiangjun PengGai‐Fang YaoZhilin ZhouFeng YangWanjie LiYuqi ZhaoYanhong LiZhuo HanXiaohong ChenHua Zhang
25
Citation
31
Reference
10
Related Paper
Citation Trend
Abstract:
Hydrogen sulfide (H2S), a novel gasotransmitter in both mammals and plants, plays important roles in plant development and stress responses. Leaf senescence represents the final stage of leaf development. The role of H2S-producing enzyme L-cysteine desulfhydrase in regulating tomato leaf senescence is still unknown. In the present study, the effect of an L-cysteine desulfhydrase LCD1 on leaf senescence in tomato was explored by physiological analysis. LCD1 mutation caused earlier leaf senescence, whereas LCD1 overexpression significantly delayed leaf senescence compared with the wild type in 10-week tomato seedlings. Moreover, LCD1 overexpression was found to delay dark-induced senescence in detached tomato leaves, and the lcd1 mutant showed accelerated senescence. An increasing trend of H2S production was observed in leaves during storage in darkness, while LCD1 deletion reduced H2S production and LCD1 overexpression produced more H2S compared with the wild-type control. Further investigations showed that LCD1 overexpression delayed dark-triggered chlorophyll degradation and reactive oxygen species (ROS) accumulation in detached tomato leaves, and the increase in the expression of chlorophyll degradation genes NYC1, PAO, PPH, SGR1, and senescence-associated genes (SAGs) during senescence was attenuated by LCD1 overexpression, whereas lcd1 mutants showed enhanced senescence-related parameters. Moreover, a correlation analysis indicated that chlorophyll content was negatively correlated with H2O2 and malondialdehyde (MDA) content, and also negatively correlated with the expression of chlorophyll degradation-related genes and SAGs. Therefore, these findings increase our understanding of the physiological functions of the H2S-generating enzyme LCD1 in regulating leaf senescence in tomato.Keywords:
Senescence
Malondialdehyde
Wild type
Cystathionine gamma-lyase
The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups.Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level. Malondialdehyde has been thought to have cytotoxic and damaging effects.Semen samples were obtained from male partners of couples requesting a fertility evaluation. Malondialdehyde was derivatized with 2-thiobarbituric acid. The malondialdehyde-2-thiobarbituric acid complex was determined by liquid chromatography with fluorescence detection. The mobile phase consisted of 20% ethanol in 25-mmol/L potassium dihydrogenphosphate (v/v), pH 6.00 ± 0.05.Analytical performance was satisfactory. Malondialdehyde levels were as follows: 1.50 ± 0.55 µmol/L in all patients, 1.40 ± 0.57 µmol/L in smokers, and 1.50 ± 0.53 µmol/L in non-smokers.The method presented here is sensitive and accurate for seminal plasma malondialdehyde determination. Our results showed a relationship between sperm motility and the malondialdehyde level in all patients and non-smokers. Malondialdehyde may induce poor sperm functionality and negatively affect the fertilization processes (Tab. 1, Fig. 1, Ref. 23).
Malondialdehyde
Thiobarbituric acid
Cite
Citations (3)
Dear Editor,
The measurement of malondialdehyde (MDA) content has long been used as a lipid peroxidation marker in studies related to oxidative stress and redox signaling, particularly in those studies focused on plant responses to abiotic and biotic stresses. A search for “malondialdehyde” and
Malondialdehyde
Cite
Citations (465)
연구목적：임신 제3삼분기에서 임신성 고혈압의 병인론으로 제기되는 산화제와 항산화제의 활성도를 평가하기 위하여 정상 임신 및 임신성 고혈압 임신부의 혈액에서 산화계는 유리기에 의한 지질과산화의 정도로 malondialdehyde, 유리기의 제거에 관여하는 항산화계는 항산화효소인 superoxide dismutase와 총 항산화제의 활성을 측정, 비교 평가하고자 하였다. 방법：21명의 건강한 정상 임신부를 대조군으로, 22명의 건강한 비 임신부와 18명의 임신성고혈압 임신부를 대상군으로 하여 임신 제 3 삼분기에서 혈액을 채취하여 산화계는 지질과산화산물인 malondialdehyde, 항산화계는 superoxide dismutase와 총 항산화제를 측정하여 비교하였다. 고혈압이 지질과산화에 미치는 영향을 알기 위하여 임신부의 malondialdehyde 농도와 수축기 및 이완기 혈압과의 상관관계를 조사하였다. 결과：Malondialdehyde는 임신성 고혈압군에서 정상 임신군보다 유의하게 증가하였다(p<0.05). Superoxide dismutase는 임신성 고혈압군에서 정상 임신군보다 유의하게 감소하였으나(p<0.05), 총 항산화제는 양 군간에 유의한 차이가 없었다. 임신부에서 혈중 malondialdehyde와 수축기 및 이완기 혈압 사이에서 높은 양의 상관관계가 있었다(r=0.475, r=0.546, p<0.05). 결론：임신성 고혈압에서 지질과산화의 최종산물인 malondialdehyde 증가와 항산화효소 특히 superoxide dismutase의 감소가 임신성 고혈압의 병태생리에 관여한다고 생각된다.
Malondialdehyde
Dismutase
Cite
Citations (0)
Hydrogen sulfide (H2S), a novel gasotransmitter in both mammals and plants, plays important roles in plant development and stress responses. Leaf senescence represents the final stage of leaf development. The role of H2S-producing enzyme L-cysteine desulfhydrase in regulating tomato leaf senescence is still unknown. In the present study, the effect of an L-cysteine desulfhydrase LCD1 on leaf senescence in tomato was explored by physiological analysis. LCD1 mutation caused earlier leaf senescence, whereas LCD1 overexpression significantly delayed leaf senescence compared with the wild type in 10-week tomato seedlings. Moreover, LCD1 overexpression was found to delay dark-induced senescence in detached tomato leaves, and the lcd1 mutant showed accelerated senescence. An increasing trend of H2S production was observed in leaves during storage in darkness, while LCD1 deletion reduced H2S production and LCD1 overexpression produced more H2S compared with the wild-type control. Further investigations showed that LCD1 overexpression delayed dark-triggered chlorophyll degradation and reactive oxygen species (ROS) accumulation in detached tomato leaves, and the increase in the expression of chlorophyll degradation genes NYC1, PAO, PPH, SGR1, and senescence-associated genes (SAGs) during senescence was attenuated by LCD1 overexpression, whereas lcd1 mutants showed enhanced senescence-related parameters. Moreover, a correlation analysis indicated that chlorophyll content was negatively correlated with H2O2 and malondialdehyde (MDA) content, and also negatively correlated with the expression of chlorophyll degradation-related genes and SAGs. Therefore, these findings increase our understanding of the physiological functions of the H2S-generating enzyme LCD1 in regulating leaf senescence in tomato.
Senescence
Malondialdehyde
Wild type
Cystathionine gamma-lyase
Cite
Citations (25)
Background: The objective is to assess oxidative stress by measuring the concentration of malondialdehyde in HIV positive children and compare it with normal children (not suffering from any disease) of the same age group.Methods: In this prospective comparative study, we analysed malondialdehyde in 80 HIV positive children in the age group of 6-12 yrs from lower socio-economic strata and compared the values with 85 normal children not infected by any disease, of the same age group and similar socio-economic strata at L.T.M.M. College. Estimation of Malondialdehyde was carried out by using the method of SADASIVUDU by thiobarbituric acid reaction.Results: The level of Malondialdehyde was significantly higher in HIV positive children than in controls.Conclusions: The increased levels of Malondialdehyde confirm the involvement of oxidative stress in the pathophysiology of this infection in children
Malondialdehyde
Thiobarbituric acid
Cite
Citations (0)
Thirty-nine hens, 3 weeks of age, were divided into 6 groups with different treatment as follows ; group A with corn oil injection, group B with corn oil injection and cholesterol feeding, group C with injection of a malondialdehyde solution, group D with injection of malondialdehyde solution and administration of elaszym, group E with injection of malondialdehyde solution and cholesterol feeding and group F with injection of malondialdehyde solution, cholesterol feeding and administration of elaszym. Corn oil and 50% malondialdehyde in corn oil were subcutaneously injected at respective doses of 0.5 and 1ml per kg body weight 12 times for 4 weeks. A diet containing 1% cholesterol without supplementary fat was fed adlibitum. Elaszym was orally administered daily at a dose of 1,500 EU per Kg body weight. A slight increase of plasma cholesterol was seen in group B. A small number of degenerate cells were seen in groups A and B. The plasma malondialdehyde levels were markedly increased in groups C and E. The result of malondialdehyde injection was a significant increase in the number of degenerate cells without stainable lipid in the abdominal aorta from group C. The feeding of a cholesterol-containing diet in combination with molon-dialdehyde injection produced numerous degenerate cells with or without stainable lipid in the abdominal aorta from group E. The administration of elaszym decreased the tissue level of malondialdehyde and the frequency of degenerate cells with or without stainable lipid in the abdominal aortas from groups D and F.
Malondialdehyde
Corn oil
Cite
Citations (0)
Vampirolepis nana is the only human tapeworm in which the intermediate host is not necessary and transmission is from person to person. In this study the changes of serum malondialdehyde level, that is, the oxidative stress hypothesis in patients infected with V. nana, was investigated.Serum malondialdehyde concentration activity was measured in 32 patients who were positive for intestinal parasite of V. nana. Levels were obtained for the positively infected patients and their age and gender were matched to 32 healthy controls.The difference between malondialdehyde levels of patients infected with V. nana and the control group was statistically significant both for females (P < 0.05) and males (P < 0.05). In the patient and control groups, no correlation was found between age and malondialdehyde levels both in females and males. In addition, no significant correlation could be found between malondialdehyde levels of both females and males for the patient and control groups.Malondialdehyde levels were clearly increased in the patients infected with V. nana.
Malondialdehyde
Cite
Citations (0)
Three tomato cultivars with different cold tolerances were used as test materials,and their seedlings'chlorophyll and malondialdehyde(MDA) variations were studied at 6,10,15℃and 25℃.The results showed that under chilling stress,the tomato leaves'chlorophyll content decreased gradually and the decrement extent was in agreement with the intensity of chilling stress,but the coldtolerant cultivar decreased less in chlorophyll content than the cold-sensitive one.The MDA content increased with the stress lasting and heightening,but the cold-tolerant cultivar increased slower and less in MDA content than the cold-sensitive one.
Malondialdehyde
Cold stress
Cite
Citations (3)
The chlorophyll content,photosynthetic rate,chlorophyll fluorescence kinetic parameters and single plant yield of a new xantha type rice mutant(Annongbiao 810S) and its wild type(Annong 810S) were comparatively studied.It was showed that: the total chlorophyll content in mutant was significantly lower than that of the wild type at the whole growth stage,the chlorophyll content in combination F1 from mutant had no difference with that in combination F1 from wild rice;the Chl a/Ch b ratio in mutant was higher than that of wild type;the net photosynthetic rate of the mutant at different stages was higher than that in the wild type;the FV/Fm,FV′/Fm′,φPS2,ETR2 and QP in the mutant were higher than that in the wild type,the QN was lower in the mutant;the yield of self-breed in the mutant was higher than that of the wild type,the test of F1 hybrid performance showed that the mutant had excellent combining ability.
Wild type
Cite
Citations (0)
Lead is widely found in our environment. Human are exposed to this metal from numerous sources, including contaminated air, water, soil and food. There are many studies that have shown that lead causes oxidative stress by inducing the generation of reactive oxygen species and reducing the anti-oxidant defense system. Lipid peroxidation increases because of impaired oxidant and anti-oxidant balance, measured by malondialdehyde levels. The current study investigates the effect of lead administration in various concentrations against lipid peroxidation. Twenty four male mice, 30-40 g body weight were divided into six groups. Group I served as control, group II to VI were given lead acetate at doses of 5, 10, 20, 40 and 80 mg/kg body weight respectively. After four weeks, plasma malondialdehyde levels and the number of erythrocytes were measured. An increase in plasma malondialdehyde levels observed in groups II to VI as compared with control, was not statistically significant (p=0,6). The increased plasma malondialdehyde levels in accordance to the increased concentration of lead administered, with the exception of group VI. The decrease in erythrocyte count observed in groups II to VI as compared with control, was not significant (p=0,1). Increased plasma malondialdehyde levels were negatively correlated with erythrocyte count (p=0,04). Lead interferes with physiological and biochemical functions related to oxidative stress. The trend to increased plasma malondialdehyde levels along with the decreased erythrocyte count as the dose of lead increased supports this statement.
Malondialdehyde
Cite
Citations (4)