Reduction wastewater discharge in second-generation acetone-butanol-ethanol (ABE) fermentation process by adsorptive removal of organic acids toward the broth recycling system
Changwei ZhangZhihao SiLihe ZhangJieyi WenChangsheng SuHuidong ChenJianbo ZhaoDi CaiXu ZhangPeiyong Qin
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To improve acetone-butanol production efficiency by Clostridium acetobutylicum,acetic and butyric acid were added to the fermentation broth to investigate the effect on cell growth and production of acetone-butanol.Addition of 1.5 g/L acetic acid increased the cell growth and enhanced acetone production,the final concentration of acetone was 21.05%,and the butanol production was not improved.Addition of 1.0 g/L butyric acid increased the cell growth and enhanced butanol production,the final concentration of butanol was 24.32%,while the acetone production was not improved.
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Effect of Acetone on Butanol Separation from Butanol-Acetone-Oleyl Alcohol Mixture by Pervaporation.
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Removing most of the water in the acetone + n-butanol + ethanol (ABE fermentation broth can reduce energy consumption and improve the biobutanol market competitiveness. Understanding the dehydration process of ethanol, acetone, and n-butanol can guide the fermentation process for the subsequent separation, which led to an investigation into the salting-out effects of K2CO3 on aqueous ethanol solution, aqueous acetone solution and n-butanol+water system at various temperatures. Elevating the salt concentration has the potential to decrease the water content of these solutes as well as the content of ethanol or acetone and n-butanol in the salt-free aqueous phase. The distribution and selectivity coefficients of ABE were also enhanced. As a result, the recovery and dehydration ratio of ethanol or acetone, or n-butanol, was also improved. The strong hydrophilicity of acetone and ethanol makes the recovery and dehydration ratio of n-butanol difficult. On the same basis(50% K2CO3), the dehydration ratios of ethanol, acetone, and n-butanol were 91.43%, 96.21%, and 97.52%, respectively. Thus, the dehydration capacity gave the following trend: n-butanol>acetone>ethanol. By raising the temperature，it was possible to increase the amount of ethanol，acetone，or n-butanol in the organic phase while decreasing their amounts in the aqueous phase. The ratio of n-butanol’s recovery and dehydration can be improved by raising the temperature.
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Acetone and butanol fermentation by Clostridium acetobutylicum GX01 using fresh potato as fermentation substrate was studied.The fermentation condition was optimized.The research results showed that the optimum fermentation condition was: the initial concentration of sugar is 90 g/L,the soybean meal is 4.2 g/L,the FeSO4.7H2O is 0.08 g/L,the fermentation temperature was 37 ℃,and the fermentation time was 60 h.The yield of butanol and total solvent under the optimum condition were 17.02 g/L and 29.39 g/L,respectively.
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