Internal Standard an Important Analyte Use in Drug Analysis by Liquid Chromatography Mass Spectrometry- An Article
2
Citation
4
Reference
10
Related Paper
Citation Trend
Abstract:
Internal standard is an external compound which is mixed with targeted analytical solution and matrix as a constant concentration and use for preparing calibration standard curve by using ratio of analyte area and internal standard area with analyte concentration and internal standard concentration. This calibration curve used for quantification of unknown concentration of anlayte of interest. This article provide necessary information about internal standard like its selection procedure, characterization, types and response factor , to all analyst who are connected with drug analysis. This article is more important and I think first article which focuses a clear idea about internal standard use in drug analysis.Keywords:
Internal standard
Standard curve
Standard addition
Matrix (chemical analysis)
To investigate the matrix effects in the residue detection of β2-agonists in pig urine and eliminating methods,pig urine samples were acidolysised by perchloric acid,extracted with ethyl acetate and methyl tertiary butyl ether,the extracts were applied to MCX cartridge for cleanup,the separation was performed by ultra performance liquid chromatography-tandem mass spectrometry.71.0%-114.5% levels of matrix effects were found in 8 β2-agonists detection.The internal standard method and matrix dilution calibration curve were compared to eliminate matrix effect.The results showed that,quantitative results of the internal standard method was 64.7%-136.0%,matrix spiked calibration curve quantitative results was 77.9%-117.9%,with 5.0mL pig urine at 0.4ng/mL,1.0ng/mL and 2.0ng/mL standard added.The quantitative matrix spiked calibration curve was more stable,and internal standard method could be replaced by matrix spiked calibration curve for quantitative.
Matrix (chemical analysis)
Standard curve
Internal standard
Residue (chemistry)
Standard addition
Cite
Citations (0)
A modification of the standard additions method called the “H-point standard additions method” is proposed in order to obtain an unbiased analyte concentration when both analyte and interferents are present in a sample. It also permits the determination of an interferent known to be present. The method uses the analytical signal data at two selected wavelengths, giving two straight lines that have a common point with coordinates H (–CH, AH), where –CH is the unknown analyte concentration and AH the analytical signal due to an interferent. Examples of the application of the proposed method are given.
Standard addition
SIGNAL (programming language)
Analytical procedures
Standard solution
Sample (material)
Internal standard
Cite
Citations (180)
OBJECTIVE To establish a rapid and accurate method for instantaneous analysis of Pb,Cd,As in blood by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). METHODS The assay method was established according to comparative study of standard curve method and standard addition curve method (a method derived and developed from standard addition method). RESULTS Both methods showed good correlative coefficients of calibration curve,limits of detection and reproducibilities for all test elements. Standard curve method,indicated unsatisfactory spiked recovery between 92.00% and 135.0%,as well as unacceptable determined values of reference materials (GBW09139e and GBW09140e). Standard addition curve method indicated satisfactory spiked recovery range from 93.33% to 102.0% and coincident contents of reference materials. CONCLUSION Standard addition curve method,bearing better spiked recovery and precise than those of standard curve method,was a rapid and accurate method for simultaneous determination of harmful elements in blood by ICP-MS.
Standard curve
Standard addition
Standard solution
Cite
Citations (0)
A liquid chromatography system with an inductively coupled plasma detector is used to prepare a single calibration curve that is useful for multiple analytes. The detector monitors the atomic emission from carbon at 193.09 nm. Hence, the analytes need not exhibit appreciable molar absorptivity or native fluorescence. Since the carbon signal is independent of molecular structure, the sensitivities for different compounds are similar as long as nebulization efficiencies are comparable. In fact, with a suitable internal standard, no calibration curve is necessary. The capability of the system is demonstrated with a test mixture of nine amino acids separated with a C30 reversed-phase column and a 20 mM phosphate buffered mobile phase. The system provides a detection limit of 30 ng carbon. A multi-analyte calibration curve is prepared with 135 distinct measurements: each of nine analytes, at five different concentrations, repeated in triplicate. The average relative standard deviation for 27 measurements of different amino acids at a given concentration is 2.5%. Clearly, a single analyte will suffice for the calibration of all nine test compounds. Similarly, the internal standard method provides an average percent error of 2.0% for the determination of 45 different amino acid concentrations using only a single replicate for each sample.
Standard curve
Molar absorptivity
Cite
Citations (19)
Internal standard is an external compound which is mixed with targeted analytical solution and matrix as a constant concentration and use for preparing calibration standard curve by using ratio of analyte area and internal standard area with analyte concentration and internal standard concentration. This calibration curve used for quantification of unknown concentration of anlayte of interest. This article provide necessary information about internal standard like its selection procedure, characterization, types and response factor , to all analyst who are connected with drug analysis. This article is more important and I think first article which focuses a clear idea about internal standard use in drug analysis.
Internal standard
Standard curve
Standard addition
Matrix (chemical analysis)
Cite
Citations (2)
Matrix (chemical analysis)
Internal standard
Standard addition
Standard curve
Quantitative Analysis
Sample Preparation
Cite
Citations (5)
Internal standard
Bioanalysis
Isotope dilution
Sample Preparation
Cite
Citations (376)
Internal standard
Standard solution
Standard addition
Cite
Citations (26)
This paper describes the determination of water content of water-soluble paints by gas chromatography. The water in paints is extracted by dimethyl formamide (DMF) as a solvent.Isopropanol is used as an internal standard. The mixture is separated by low-speed centrifugation.Then a 1-uL sample of the supernatant from the prepared solution is injected into the gas chromatograph. The water content is determined by internal standard calibration curve. The rate of recovery of added standard of this method is more than 98%. Relative mean deviation is less than 3‰.The linearity of calibration curve is good and relativity coefficient is higher than 0.998.
Standard curve
Relative standard deviation
Internal standard
Cite
Citations (0)
The standard solutions prepared by different methods to establish the amount of external standard calibration curve recovery compared to the preparation of acetone solvent matrix calibration curve standard solution of chlorpyrifos recovery is 92%to 99%.relative standard deviation(RSD)≤4.18%,chlorpyrifos in the 0.035～1.4 mg/L in the range a good linear relationship between the instrument detection limit of this method is 0.02 mg/L,the detection limit is 0.008 mg/kg.On the same sample by double flame photometric detector(FPD)detection, DB-1701+DG than the DB-1 column matrix effects a small column.Standard solution prepared by the solvent matrix to establish the amount of external standard calibration curve to eliminate matrix effects.
Matrix (chemical analysis)
Standard solution
Standard curve
Standard addition
Cite
Citations (0)