miR-139-5p Regulates the Proliferation of Acute Promyelocytic Leukemia Cells by Targeting MNT
Yueyue FuLimin LiJinxiao HouHuibo LiChengfang LvHongjuan YuXiaoqian ZhangMengyuan XuMingwen ZhangHongbin MengJie LiuXin LianJia‐Wei FengJin Zhou
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Abstract:
Acute promyelocytic leukemia (APL) patients with progressive leukocytosis are more likely to have various complications and poor outcomes. However, the regulatory roles of microRNAs in the leukocytosis of APL have not been clarified. Our study aims to evaluate the effects of miRNAs on leukocytosis during induction therapy of APL patients and explore its potential mechanisms. During induction treatment, patients with white blood cell count higher than 10 × 109/L were divided into leukocytosis group and others were nonleukocytosis group. Using microarray assays, we found that miR-139-5p was significantly downregulated in the leukocytosis group. Elevated expression of miR-139-5p inhibited the proliferation of NB4 cells by arresting the cell cycle and inducing apoptosis. We further identified that MNT was a target of miR-139-5p. miR-139-5p significantly inhibited the proliferation, invasion, and migration function of NB4 cells through targeting MNT. Strategies for regulating miR-139-5p or MNT expression might provide new therapeutic approaches for progressive leukocytosis in APL.Keywords:
Leukocytosis
White blood cell
Leukocytosis
White blood cell
Erythrocyte sedimentation rate
Foot (prosody)
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Leukocytosis
Eosinopenia
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Objective To investigate the clinical significance of leukocytosis. MethodsWith the analysis of blood test,we summed up the importance of white blood cell count(WBC) and the clinical significance of leukocytosis. ResultsWBC count was of high clinical value,to which clinicians could refer.Leukocytosis is mainly seen in infection and leukemoid reaction. Conclusions The result of WBC test,to a certain extent,determines the clinical diagnosis and treatment of leukocytosis.
Leukocytosis
Leukemoid reaction
Clinical Significance
White blood cell
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Arsenic Trioxide (As2O3) is an effective agent for treating acute promyelocytic leukemia achieving a complete remission rate of about 60% to 90%. It is similar to all-trans retinoic acid (ATRA) when treating acute promyelocytic leukemia (APL), because both agents have limited side effects compared to conventional chemotherapy, although the treatment period is more prolonged. During treatment, both agents may induce leukocytosis, and in patients taking ATRA, leukocytosis appears to be related to the development of retinoic acid syndrome (RAS). We report here a case of APL treated with ATRA in combination with chemotherapy 3 years earlier. During treatment, an episode of RAS with fever, edema, pericardiac effusion etc. was encountered. Recently, she had a relapse of leukemia, and As2O3 therapy was used. Leukocytosis developed again, and symptoms of fever, skin rash, edema resembling a RAS also developed, which was quickly relieved by steroid administration in a manner resembling response to RAS.
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In this study we measured white blood cells count and haematoccrits of 300 subjects. The study was divided into two groups. Group A constituted the controls of 100 subjects with normal leukocyte counts. The group B constituted the study group with a sample size of 200 subjects. The study group showed an elevated white blood cells count (leukocytosis). The average mean age of group A and B were 39±19 and 41±20 respectively. The haematocrit was measured using the microhaematocrit method, whereas the white cell count was estimated using the manual Neubauer method. The group A had a mean haematocrit and white cell count value of 35±8 and 7.1±1.6 respectively. Whereas the group B had a mean haematocrit and white cell of 29±10 and 22.2±15.8 respectively. The results showed a significant decrease (p<0.05) in haematocrit of the study group (group B) as compared against the control group (group A) using student t test. This study had satisfied that a relationship do exist between haematocrit and leukocytosis. Hence, patients diagnosed of leucocytosis be placed on blood boosting therapy and diet to avoid anaemia. Keywords: Leukocytosis, haematocrit, anaemia, superoxide
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Acute abdominal symptoms in 162 children were investigated with rapidly quantified C-reactive protein (CRP), white blood cell (WBC) count and erythrocyte sedimentation rate (ESR) as laboratory parameters. When the symptoms had been present for at least 12 hours, the CRP level was greater than 10 mg/l in 72% of cases with histologic evidence of appendicitis, while leukocytosis (WBC greater than 15 000/mm3) and raised ESR (greater than 20 mm/l h) were found in 58% and 51%, respectively. If appendicitis had progressed to gangrenous stage, however, CRP alone was capable of identifying 83% of cases, while 76% had leukocytosis and 60% elevated ESR. When perforation had occurred there was greatly increased CRP and invariably leukocytosis, but elevated ESR in only 60%. With the combination CRP and WBC count (using rise in one or both), 88% of all appendicitis cases could be identified, and at least 96% of those with gangrene or perforation. The predictive value of combined positive CRP and WBC tests was not less than 93%.
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Pseudohypoxemia has been reported in leukemic patients with extreme leukocytosis, and it is characterized by a low oxygen saturation on arterial blood gas analysis despite normal saturation on pulse oximetry. We report the case of a 51-year-old man with chronic lymphocytic leukemia and an elevated white blood cell (WBC) count after splenectomy, his progressive postoperative pseudohypoxemia gradually improved as the leukocytosis was lowered by chemotherapy. We believe this is the first report to show a statistically significant correlation between the WBC count and the degree of pseudohypoxemia in a patient with leukemia.
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White blood cell
Pulse Oximetry
Oxygen Saturation
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