DETERMINATION OF ANTIMICROBIAL ACTIVITY OF CHLORHEXIDINE GEL.
Lina BabickaitėKristina RamanauskienėAidas GrigonisMarija IvaškienėGintaras DaunorasIrena KlimienėMarius VirgailisGintaras ZamokasAsta Marija InkėnienėAlgimantas Petras Matusevičius
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Abstract:
Many researchers have advocated in recent times that antiseptic use in healing wounds should be discouraged. Antiseptics have been found to retard healing of wounds. Poloxamer 407 shows thermoreversible properties, which are of the utmost interest in optimizing drug formulation (fluid state at room temperature facilitating administration and gel state above sol-gel transition temperature, at body temperature, promoting prolonged release of pharmacological agents). Chlorhexidine, a commonly used antiseptic, is known to be less toxic on granulation cells. Acting as an antiseptic, it is an effective bactericidal agent against the most categories of microbes, including bacteria, yeast, and viruses. Objective of this study was to evaluate antimicrobial activ- ity of chlorhexidine containing poloxamer gel to Gram-positive and Gram-negative bacteria in vitro. Chlorhexidine gels and chlorhexidine aqueous solutions have different antibacterial activity to S. amis, E.faecalis, E. coli and P. aemginosa strains in vitro. It depends on concentration and dosage form of antiseptic. Study results confirmed that antimicrobial activity of gel depends on active ingredient concentration in antiseptic. The best inhibition effect for both of reference and wild-type bacteria was obtained for 1% chlorhexidine gel. Summarizing the results and assessing the characteristics of the gel ingredients, it can be suggested using chlorhexidine gels in veterinary medicine.Keywords:
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Many researchers have advocated in recent times that antiseptic use in healing wounds should be discouraged. Antiseptics have been found to retard healing of wounds. Poloxamer 407 shows thermoreversible properties, which are of the utmost interest in optimizing drug formulation (fluid state at room temperature facilitating administration and gel state above sol-gel transition temperature, at body temperature, promoting prolonged release of pharmacological agents). Chlorhexidine, a commonly used antiseptic, is known to be less toxic on granulation cells. Acting as an antiseptic, it is an effective bactericidal agent against the most categories of microbes, including bacteria, yeast, and viruses. Objective of this study was to evaluate antimicrobial activ- ity of chlorhexidine containing poloxamer gel to Gram-positive and Gram-negative bacteria in vitro. Chlorhexidine gels and chlorhexidine aqueous solutions have different antibacterial activity to S. amis, E.faecalis, E. coli and P. aemginosa strains in vitro. It depends on concentration and dosage form of antiseptic. Study results confirmed that antimicrobial activity of gel depends on active ingredient concentration in antiseptic. The best inhibition effect for both of reference and wild-type bacteria was obtained for 1% chlorhexidine gel. Summarizing the results and assessing the characteristics of the gel ingredients, it can be suggested using chlorhexidine gels in veterinary medicine.
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Aim: This study was conducted to evaluate the antimicrobial effect of propolis extract with different dilutions when applied as mouth wash in vivo against a known antiseptic agent (0.2%Chlorhexidine) . Material and Methods: Ninety children were selected for this study. All children were examined clinically for dental caries and used to evaluate the antimicrobial effect of studied materials. The childern were divided equally into two groups (fourty five in every group). In the first group childern were instructed to rinse with 3 ml of propolis extract for specific time and divided into three sub groubs while in the other group childern were instructed to rinse with 3 ml of Chlorhexidine. Result: The results of this study revealed that the use of propolis mouth washby childern achieved mean of reduction of total bacterial count. The effect of 0.2% Chlorhexidine was higher than the effect of propolis in total bacterial count reduction .Conclusion: This study concluded that Propolis is an antimicrobial agent as it significantly reduced the total bacterial count in the saliva of childern when compared by apotent antiseptic like 0.2% chlorhexidine.
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Bacteria grown in biofilms are less susceptible to antimicrobial agents than planktonic bacteria. One of the most common biofilms in humans is dental plaque. To investigate the antimicrobial activity against dental bacteria grown in biofilms, biofilms were generated with Streptococcus sanguis on hydroxyapatite disks to mimic the tooth surface. After 48 h of aerobic incubation at 37 degrees C in a continuous flow culture, a biofilm consisting of Streptococcus sanguis became visible on the surface of the disks. The disks were removed from the growth chamber and placed in different vessels containing either chlorhexidine digluconate (CAS 55-56-1, 0.1% or 1.0%), polyvinylpyrrolidone iodine (1.5% or 7.5%), or octenidine dihydrochloride (CAS 70775-75-6, 0.05% or 0.1%) for 5 or 30 min. In addition, the antiseptics were applied to the bacterial suspension in the growth chamber. A significant difference was observed in the antimicrobial activity against bacteria in the suspension liquid compared to the bacteria grown in biofilms. The best reduction factors were obtained with chlorhexidine (1.0%, 30 min) for either sessile (3.97 Ig) or planktonic bacteria (> or = 5.58 Ig). Clear relationships between the doses and times of action were found for the assessed agents. Therefore, the authors conclude that the present in vitro assay is a quick and cost-effective model to screen the activity of antimicrobial agents against bacteria grown in biofilms.
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Abstract: Skin and mucosal infections constitute recurrent pathologies resulting from either inappropriate antiseptic procedures or a lack of efficacy of antimicrobial products. In this field, nanomaterials offer interesting antimicrobial properties (eg, long-lasting activity; intracellular and tissular penetration) as compared to conventional products. The aim of this work was to produce, by a new solvent-free process, a stable and easily freeze-dryable chlorhexidine-loaded polymeric nanocapsule (CHX-NC) suspension, and then to assess the antimicrobial properties of nanomaterials. The relevance of the process and the physicochemical properties of the CHX-NCs were examined by the assessment of encapsulation efficiency, stability of the nanomaterial suspension after 1 month of storage, and by analysis of granulometry and surface electric charge of nanocapsules. In vitro antimicrobial activities of the CHX-NCs and chlorhexidine digluconate solution were compared by measuring the inhibition diameters of two bacterial strains ( Escherichia coli and Staphylococcus aureus ) and one fungal strain ( Candida albicans ) cultured onto appropriate media. Based on the findings of this study, we report a new solvent-free process for the production of nanomaterials exhibiting antimicrobial activity, suitable stability, and easily incorporable as a new ingredient in various pharmaceutical products. Keywords: nanomaterial, nanocapsules, antiseptic, chlorhexidine, solvent-free process
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The recent increase in infections mediated by drug-resistant bacterial and fungal pathogens underlines the urgent need for novel antimicrobial compounds. In this study, the antimicrobial activity (inhibitory and cidal) of HybenX®, a novel dessicating agent, in comparison with commonly used sodium hypochlorite and chlorhexidine, against a collection of bacterial and yeast strains representative of the most common human pathogenic species was evaluated. The minimal inhibitory, bactericidal, and fungicidal concentrations (MIC, MBC, and MFC, respectively) of the three different antimicrobial agents were evaluated by broth microdilution assays, followed by subculturing of suitable dilutions. HybenX® was active against 26 reference strains representative of staphylococci, enterococci, Enterobacterales, Gram-negative nonfermenters, and yeasts, although at higher concentrations than sodium hypochlorite and chlorhexidine. HybenX® MICs were 0.39% for bacteria (with MBCs ranging between 0.39% and 0.78%), and 0.1-0.78% for yeasts (with MFCs ranging between 0.78% and 1.6%). HybenX® exhibited potent inhibitory and cidal activity at low concentrations against several bacterial and yeast pathogens. These findings suggest that HybenX® could be of interest for the treatment of parodontal and endodontic infections and also for bacterial and fungal infections of other mucous membranes and skin as an alternative to sodium hypochlorite and chlorhexidine.
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During an outbreak of Serratia marcescens infections at our hospital, we discovered widespread contamination of the 2% chlorhexidine hand-washing solution by S. marcescens. Examination by electron microscopy of the sides of bottles in which this solution was stored revealed that microorganisms were embedded in a fibrous matrix. Bacteria, free in the liquid, were morphologically abnormal, showing cell wall disruption or cytoplasmic changes. Furthermore, bacteria adherent to the walls of the storage jugs and embedded in this fibrous matrix also had morphologically abnormal cytoplasm. Despite these changes, viable S. marcescens organisms were recovered from the fluid during a storage period of 27 months. The concentration of chlorhexidine required to inhibit these strains of Serratia was 1,024 microgram/ml; however, the organism could survive in concentrations of up to 20,000 micrograms/ml. Additional studies are needed to define the mechanism(s) that allows such bacteria to contaminate and survive in disinfectants.
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Objectives: The study aimed to evaluate the action, in vitro, of hydrogen peroxide in the concentrations of 1%, 1.5% and 3%, of cetylpyridinium chloride, of benzalkonium chloride of 1.3% and of chlorhexidine 0.12% associated with 1.5% hydrogen peroxide over standardized strains of microorganisms Enterococcus faecalis; Staphylococcus aureus; Candida albicans and Escherichia coli, aspiring to determine which is the best product. Chlorhexidine 0.12% was used as a positive control and sterile distilled water as a negative control. Methodology: Standardized suspensions of the microorganisms were prepared, poured and spread over the culture media. Sterile 4 mm paper discs were placed on the seeded agar and soaked with 15 microliters (uL) of each of the antiseptics. The plates were incubated in a bacteriological oven at 37º Celsius for 48 hours. The results were obtained through the growth inhibition halo. Results: The antiseptic that obtained the greatest antimicrobial action was the association of hydrogen peroxide at 1.5% with chlorhexidine at 0.12%, followed by benzalkonium chloride 1.3%. Hydrogen peroxide at 1% did not show any antimicrobial activity. Conclusions: The 0.12% chlorhexidine associated with 1.5% hydrogen peroxide demonstrated the best antimicrobial action, indicating a possible synergistic action between the products, increasing the efficiency when compared to the isolated use of each active ingredient. Benzalkonium chloride 1.3%, also showed an effective antimicrobial action, indicating that these products or combinations have a great potential to be used as an active ingredient in mouthwashes.
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