[A clinical analysis of children with invasive pulmonary fungal infections after biliary atresia surgery].
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Abstract:
To investigate the clinical features of invasive pulmonary fungal infections (IPFIs) after biliary atresia (BA) surgery and related risk factors.A retrospective analysis was performed for the clinical data of 49 children with IPFIs after BA surgery, including clinical features, lung imaging findings, and pathogenic features. The risk factors for IPFIs after BA surgery were also analyzed.The most common pathogens of IPFIs after BA surgery was Candida albicans (17 strains, 45%), followed by Candida tropicalis (7 strains, 18%), Aspergillus (6 strains, 16%), Candida krusei (3 strains, 8%), Candida glabrata (3 strains, 8%), and Candida parapsilosis (2 strains, 5%). Major clinical manifestations included pyrexia, cough, and shortness of breath, as well as dyspnea in severe cases; the incidence rate of shortness of breath reached 78%, and 35% of all children had no obvious rale. The multivariate logistic regression analysis showed that age at the time of surgery, time of glucocorticoid application, cumulative time of the application of broad-spectrum antibiotics, and recurrent cholangitis were major risk factors for IPFIs after BA surgery.The three most common pathogens of IPFIs after BA surgery are Candida albicans, Candida tropicalis, and Aspergillus. It is important to perform surgery as early as possible, avoid recurrent cholangitis, and shorten the course of the treatment with broad-spectrum antibiotics and glucocorticoids for decreasing the risk of IPFIs.Keywords:
Candida parapsilosis
Candida krusei
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Non-albicans Candida (NAC) species are emerging as important opportunistic pathogens. This has significant clinical impact as, NAC species have decreased susceptibility to commonly used antifungal agents. Hence species identification in the Clinical Microbiology laboratory is essential. This study was conducted to assess efficacy of HiCrome agar to reliably identify Candida to the species level. Altogether 48 isolates of Candida were isolated during a period of one year constituting 24 isolates of Candida albicans, 13 of Candida tropicalis, 4 of Candida krusei, 4 of Candida glabrata, 2 of Candida parapsilosis and 1 isolate of Candida guillermondii. Identification was done based on microscopic morphology, germ tube test, growth at 45°C, morphology on corn meal agar and colony colour on HiCrome agar. HiCrome agar accurately identified all species of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata. Two isolates of Candida parapsilosis and one isolate of Candida guillermondii was misidentified as Candida glabrata. HiCrome agar can be used as a fairly reliable and time saving alternative to conventional methods with good sensitivity and specificity.
Candida krusei
Candida parapsilosis
Candida glabrata
Germ tube
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The molecular detection of Candida plays an important role in the diagnosis of candidaemia, a major cause of morbidity and mortality. The sensitivity of this diagnosis is partly related to the efficiency of yeast DNA extraction. In this monocentric study, we investigated the suitability of 11 recent automated procedures for the extraction of low and high amounts of Candida DNA from spiked blood. The efficacy of the DNA extraction procedures to detect Candida spp. in blood samples ranged from 31.4% to 80.6%. The NucliSENSTM easyMAGTM procedure was the most efficient, for each species and each inoculum. It significantly outperformed the other procedures at the lower Candida inocula mimicking the clinical setting. This study highlighted a heterogeneity in DNA extraction efficacy between the five main Candida species (Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis and Candida krusei). Up to five automated procedures were appropriate for C. krusei DNA extraction, whereas only one method yielded an appropriate detection of low amount of C. tropicalis. In the era of the syndromic approach to bloodstream infection diagnosis, this evaluation of 11 automated DNA extraction methods for the PCR diagnosis of candidaemia, puts the choice of an appropriate method in routine diagnosis within the reach of laboratories.
Candida krusei
Candida parapsilosis
Candida glabrata
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The specie Cissus verticillata (L.) Nicolson & C. E. Jarvis subsp. verticillata is known as Vegetable insulin and is used in popular medicine for the treatment of diabetes mellitus. The diabetic patient has a greater risk of urinary infections, and Candida spp. is the main gender involved. Was evaluated, the antifungal activity of the hydroalcoholic extract and its fractions against Candida albicans ATCC 18804, Candida krusei ATCC 6258, Candida parapsilosis ATCC 22019 and Candida tropicalis ATCC 750. The results indicated that leaves of C. verticillata verticillata has promising potential antifungal in fractions diclormetano and chloroform, with minimum inhibitory concentration of 125 µg/mL in C. krusei and C. tropicalis respectively. We need new tests with chemical constituents of these fractions isolated, seeking higher activity in inhibiting the growth of Candida spp. Resumo: A especie Cissus verticillata (L.) Nicolson & C. E. Jarvis subsp. verticillata e conhecida como Insulina vegetal e utilizada na medicina popular no tratamento da diabetes mellitus. O paciente diabetico tem maior risco de infeccoes urinarias e Candida spp. e o principal genero envolvido. Avaliou-se a atividade antifungica do extrato hidroalcoolico e de suas fracoes frente a Candida albicans ATCC 18804, Candida krusei ATCC 6258, Candida parapsilosis ATCC 22019 e Candida tropicalis ATCC 750. Os resultados obtidos indicaram que folhas de C. verticillata verticillata tem potencial antifungico promissor nas fracoes diclorometano e cloroformio, com concentracao inibitoria minima de 125 µg/mL em C. krusei e em C. tropicalis respectivamente. Sao necessarios novos testes com constituintes quimicos isolados dessas fracoes, buscando atividade superior na inibicao do crescimento de Candida spp. Palavras-chave: Candida spp; Cissus verticillata; Atividade antifungica
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Candida parapsilosis
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Background: The incidence of systemic fungal infections caused by non-albicans species is increasing. Colonization with Candida has been identified as an independent risk factor for invasive Candidiasis. Methods: In a prospective study for 30 month, we analysed samples weekly over a period of four weeks from an initial count of 411 patients (mean APACHE-II-Score 20.8) admitted to our ICU. Swabs from nostril, throat and anus and specimens of tracheal secretions and urine were taken and cultured on CHROM- or CandID- Agar at 36 °C. Results: 41 of the patients in the study stayed in the ICU for at least 4 weeks. Of these, 24 received systemic antimycotics (mean duration 15.9 days) for proven or probable fungal infection. In the untreated group, Candida species were cultured from 29% of specimens at baseline (71% Candida albicans, 13% Candida glabrata, 17% Candida tropicalis, 4% Candida parapsilosis, 0% Candida krusei) and in 42% after 4 weeks (66% Candida albicans, 16% Candida glabrata, 0% Candida tropicalis, 16% Candida parapsilosis, 0% Candida krusei). In the group with systemic antimycotic therapy, Candida species were cultured from 66% of specimens at baseline (59% Candida albicans, 46% Candida glabrata, 6% Candida tropicalis, 3% Candida parapsilosis, 11% Candida krusei) and in 39% after 4 weeks (29% Candida albicans, 60% Candida glabrata, 0% Candida tropicalis, 13% Candida parapsilosis, 7% Candida krusei). Conclusions: Antimycotic therapy results in a reduction in Candida colonization. This is due to a decrease in the fraction of Candida albicans whereas Candida glabrata is left unchanged. In the absence of antimycotics, Candida colonization increase and the fractions of Candida albicans and Candida glabrata remain unchanged.
Candida krusei
Candida parapsilosis
Candida glabrata
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Ojbective: To investigate the etiological characteristics of deep infection caused by candida.Methods: The routine methods use for identifying Candida included: inoculate on CHROMagar and Corn-Tween 80 agar、 gemma-form test、 commercial kit included API 20C AUX and VITEK YBC card、 blood culture using VITAL.Results: From May 2004 to August 2007,we separated 749 strains of Candida from deep infected specimens.The ratio of different Candida were: Candida albicans 41.79%、 Candida tropicalis 24.17%、 Candida glabrata 12.02%、 Candida parapsilosis 12.95%、 Candida krusei 2%、 other Candida 7.07%.Conclusion: Candida albicans is the most common type,but its ratio is decreasing compared with the past.The ratios of Candida glabrata and Candida parapsilosis are increasing significantly.
Candida krusei
Candida parapsilosis
Candida glabrata
Candida infections
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Candida stain is a conditional pathomycete that could be isolated from the bovine with the disease of endometritis.The 183 Candida strains were isolated from the uterus mucus of bovine with disease endometritis were used to identified at the level of generic by API20CAUX.The most commonly could be isolated were Candida krusei which accounted for 33.9%,followed by Candida rugosa were 17.5%,and the Candida kefyr,Candida albicans,Candida tropicalis were 13.1%,11.5%,8.7%.The seldom could be isolated were Candida zeylanoides,Candida parapsilosis,Candida guilliermondi,Candida fanata,Candida glabrata were 5.5%,4.4%,3.3%,1.1%,1.1%.In addition,we had also tested the active of hemolyzation in vitro of the Candida stain.Candida krusei,Candida kefyr,Candida albicans,Candida tropicalis,Candida zeylanoides and Candida glabrata display α and β haemolysis post inoculation 48 h.Candida rugosa,Candida guilliermondi,Candida fanata only display α haemolysis,Candida parapsilosis didn't display any haemolysis after incubation 72 h.Candida albicans and Candida kefyr had a higher haemolysis than others.This study lays the foundation for future study of clinical prevention and treatment of fungal endometritis and pathogenesis.
Candida krusei
Candida parapsilosis
Candida glabrata
Candida dubliniensis
Candida rugosa
Haemolysis
Trichosporon
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Due to the limitations of classical methods for the detection of systemic fungal infections and the high mortality rates associated with these infections, it has become essential to develop a quick, sensitive and specific detection assay. By using the Idaho Technologies Light-Cycler system, a qualitative real-time PCR system has been developed for the detection of the leading causes of systemic infection within the genus Candida. The sensitivity of the assay was comparable to previously described PCR methods (1-5 c.f.u. ml(-1)) and, by the use of a single Candida probe, it was able to detect, but not differentiate between, seven species of Candida (Candida albicans, Candida dubliniensis, Candida glabrata, Candida kefyr, Candida krusei, Candida parapsilosis and Candida tropicalis). Single-round amplification on the Light-Cycler allowed rapid turn-around of clinical samples (within one working day) and it was shown to be more sensitive than classical procedures, exposing 39 possible systemic infections that were not detected by blood culture.
Candida krusei
Candida dubliniensis
Candida parapsilosis
Candida glabrata
Candida infections
Systemic candidiasis
Fungemia
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The specie Cissus verticillata (L.) Nicolson & C. E. Jarvis subsp. verticillata is known as Vegetable insulin and is used
in popular medicine for the treatment of diabetes mellitus. The diabetic patient has a greater risk of urinary infections, and Candida spp. is the main gender involved. Was evaluated, the antifungal activity of the hydroalcoholic extract and its fractions against Candida albicans ATCC 18804, Candida krusei ATCC 6258, Candida parapsilosis ATCC 22019 and Candida tropicalis ATCC 750. The results indicated that leaves of C. verticillata verticillata has promising potential antifungal in fractions diclormetano and chloroform, with minimum inhibitory concentration of 125 μg/mL in C. krusei and C. tropicalis respectively. We need new tests with chemical constituents of these fractions isolated, seeking higher activity in inhibiting the growth of Candida spp.
Candida krusei
Candida parapsilosis
Candida glabrata
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Candida parapsilosis
Candida glabrata
Candida krusei
Candida dubliniensis
Candida infections
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Candida krusei
Candida parapsilosis
Candida glabrata
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Citations (56)