Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)
Ellen DecatEls Van MechelenBart SaerensStefan VermeulenTeun BoekhoutSteven De BlaiserMario VaneechouttePieter Deschaght
27
Citation
29
Reference
10
Related Paper
Citation Trend
Keywords:
Candida parapsilosis
Candida glabrata
Candida krusei
Candida dubliniensis
Candida infections
Candida yeasts are normal commensal of human microbiota, which can cause infection in patients with predisposing factors. Photodynamic antimicrobial therapy has been employed against several microbial infections. Light, when associated with dyes, promotes microbial death in a less invasive way, reducing the treatment time. The aim of the present study was to investigated the effect of Photodynamic Therapy (PDT) with MetyleneBlue (MB) and a light emitting diode (LED), on Candida species.Cultures of C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. krusei and C. dubliniensis weresubmmited to photodynamic therapy employing LED and Methylen blue. The number of CFU/mL was reduced in 100% for Candida glabrata , 99.57% for Candida albicans , 99.41% for Candida krusei , 97.53% for Candida dubliniensis , 97.29% for Candida tropicalis , and 96.88% for Candida parapsilosis when compared with the control group. The results showed that the PDT significantly reduced the CFU of Candida . The result of this study allows us to conclude that all Candida species tested were susceptible to the PDT treatment.
Candida dubliniensis
Candida krusei
Candida glabrata
Candida parapsilosis
Cite
Citations (0)
Objective:To investigate the etiological features and relevant risk factors of Candida infections in hospital.Methods:1170 patients with Candida infections admitted in the Second Hospital of Shanxi Medical University during April 2006 to March 2008 were enrolled in this investigation,a prospective case-control study during 1170 cases of Candida infections and 1220 cases of non-Candida infections.The incidence and risk factors of Candida infections were analyzed by method of SPSS13.0 model.Results:(1) 1258 cases were isolated from different samples,and Candida albicans 895(71.14%) was the largest,followed by Candida glabrata 10.73%,Candida krusei 10.26%,Candida tropicalis 3.42%,Candida parapsilosis 2.78%,Candida lusitaniae 1.67%.(2) Univariate analysis and multivariate logistic regression analysis showed that sex,age,pathogen detection time,underlying disease,albumin,prophylaxisly antifungal drugs using,immunosuppressants using,invasive examination and treatment(such as total parenteral nutrition(TPN),invasive procedures,central venous catheters,hemodialysis and mechanical ventilation,et al.) were the independent risk factors for Candida infections.Conclusion:Candida albicans is the main pathogens of Candida infections in patients.To efficiently control the risk factors should be emphasized,including early diagnosis and treatment underlying diseases and comorbidities,appropriate use tools of examine and prudent use methods of treatment.
Candida krusei
Candida parapsilosis
Candida glabrata
Candida infections
Univariate analysis
Cite
Citations (0)
Novel highly functionalized bis cyclohexenone ethyl carboxylates 7-12 were designed, synthesized and their structures were elucidated by their elemental analysis, MS, FT-IR, one-dimensional 1H, and 13C NMR spectroscopic data.All the synthesized compounds 7-12 were tested for their in vitro antifungal activities against Candida sp. namely Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis, Candida dubliniensis and Candida krusei.A close inspection of the in vitro anticandidal activity profile in differently electron withdrawing (-F, -Cl, and -Br) functional group and electron donating (CH3 and OCH3) substituted phenyl rings of novel highly functionalized bis cyclohexenone ethyl carboxylates 7-12 exerted strong anticandidal activity against all the tested Candida species. All the synthesized compounds 7-12 exhibited MIC value in the range of 6.25-200 μg/mL against all the tested Candida (C.) species.Compound 8 against C. albicans, 9,11 against C. glabrata, 8,10 against C. parapsilosis, 7,9 against C. dubliniensis, 8,10 against C. krusei exhibited excellent anticandidal activity at a MIC value of 6.25 μg/mL. Likewise compound 7, 9-11 against C. albicans, 8, 9, 11 against C. tropicalis, 8 against C. glabrata, 9 against C. parapsilosis, 10 against C. dubliniensis, 9 against C. krusei revealed superior activity at a MIC value of 12.5 μg/mL.
Candida krusei
Candida parapsilosis
Candida dubliniensis
Candida glabrata
Cite
Citations (2)
Candida species are common causes of disease ranging from superficial cutaneous and mucocutaneous infections to invasive infections such as candidemia and disseminated candidiasis. There are more than 150 species of Candida, but only 9 are frequent human pathogens. The most common isolate is Candida albicans (Figure 170.1); other encountered pathogens include Candida tropicalis (Figure 170.2), Candida parapsilosis, Candida glabrata, Candida krusei (Figure 170.3), Candida kefyr, Candida lusitaniae, Candida dubliniensis, and Candida gulliermondii. Less commonly isolated species with medical significance include Candida lipolytica, Candida famata, Candida rugosa, Candida viswanathii, Candida haemulonii, Candida norvegensis, Candida catenulate, Candida ciferri, Candida intermedia, Candida utilis, Candida lambica, Candida pulcherrima, and Candida zeylanoides. Most species are commensal organisms, colonizing the skin, gastrointestinal tract, and vagina, and they become opportunistic pathogens only when the host has compromised immunologic or mechanical defenses or when there are changes in the host's normal flora, such as those triggered by broadspectrum antibiotic use.
Candida parapsilosis
Candida krusei
Candida dubliniensis
Candida glabrata
Candida infections
Candida rugosa
Cite
Citations (1)
Candida stain is a conditional pathomycete that could be isolated from the bovine with the disease of endometritis.The 183 Candida strains were isolated from the uterus mucus of bovine with disease endometritis were used to identified at the level of generic by API20CAUX.The most commonly could be isolated were Candida krusei which accounted for 33.9%,followed by Candida rugosa were 17.5%,and the Candida kefyr,Candida albicans,Candida tropicalis were 13.1%,11.5%,8.7%.The seldom could be isolated were Candida zeylanoides,Candida parapsilosis,Candida guilliermondi,Candida fanata,Candida glabrata were 5.5%,4.4%,3.3%,1.1%,1.1%.In addition,we had also tested the active of hemolyzation in vitro of the Candida stain.Candida krusei,Candida kefyr,Candida albicans,Candida tropicalis,Candida zeylanoides and Candida glabrata display α and β haemolysis post inoculation 48 h.Candida rugosa,Candida guilliermondi,Candida fanata only display α haemolysis,Candida parapsilosis didn't display any haemolysis after incubation 72 h.Candida albicans and Candida kefyr had a higher haemolysis than others.This study lays the foundation for future study of clinical prevention and treatment of fungal endometritis and pathogenesis.
Candida krusei
Candida parapsilosis
Candida glabrata
Candida dubliniensis
Candida rugosa
Haemolysis
Trichosporon
Cite
Citations (0)
A total of 50 HIV seropositive patients with oral lesions/lesions suggestive of oral candidiasis were studied. Their HIV status was determined by 1 ELISA and 2 ELISA/RAPID/SIMPLE Tests in Department of Microbiology, Govt. Medical College, Amritsar. Oral swabs were collected from these patients. Candida species were isolated from 32 patients. The isolates were speciated by standard techniques. Of these, 20 isolates were identified as Candida albicans, 3 as Candida dubliniensis. 3 were Candida krusei and one each was Candida glabrata. Candida tropicalis. Candida kefyr, Candida parapsilosis, Candida albicans var stellatoidea and Candida guilliermondii.
Candida dubliniensis
Candida krusei
Candida parapsilosis
Candida glabrata
Cite
Citations (1)
Due to the limitations of classical methods for the detection of systemic fungal infections and the high mortality rates associated with these infections, it has become essential to develop a quick, sensitive and specific detection assay. By using the Idaho Technologies Light-Cycler system, a qualitative real-time PCR system has been developed for the detection of the leading causes of systemic infection within the genus Candida. The sensitivity of the assay was comparable to previously described PCR methods (1-5 c.f.u. ml(-1)) and, by the use of a single Candida probe, it was able to detect, but not differentiate between, seven species of Candida (Candida albicans, Candida dubliniensis, Candida glabrata, Candida kefyr, Candida krusei, Candida parapsilosis and Candida tropicalis). Single-round amplification on the Light-Cycler allowed rapid turn-around of clinical samples (within one working day) and it was shown to be more sensitive than classical procedures, exposing 39 possible systemic infections that were not detected by blood culture.
Candida krusei
Candida dubliniensis
Candida parapsilosis
Candida glabrata
Candida infections
Systemic candidiasis
Fungemia
Cite
Citations (109)
Candida parapsilosis
Candida krusei
Candida glabrata
Candida infections
Cite
Citations (103)
Candida parapsilosis
Candida glabrata
Candida krusei
Candida dubliniensis
Candida infections
Cite
Citations (27)
A novel triterpenoidal compound named 'amnomopin' (3β-diglucoside-5,12-28-oic acid), which is named IUPAC as 3-O-(2' ➔ 1″diglucoside)1,2,3,4,4a,5,6,6a,6b,7,9,10,11,12,12a,12b,13,14b-octadecahydro-10-hydroxy-2,2,6a,6b,9,9,12a-heptamethylpicene-4a-carboxylic acid, was isolated from the extract Petriella setifera. The total alcoholic extract of P. setifera showed a great activity against clinically isolated Candida species, including Candida albicans, Candida dubliniensis, Candida famata, Candida glabrata, Candida inconspicua, Candida kefyr, Candida krusei, Candida norvegensis, Candida parapsilosis and Candida tropicalis. Also, the new compound amnomopin was active against all the investigated Candida species. The highest anticandidal activity of P. setifera extract was obtained against C. kefyr (22.6 ± 1.5 mm), C. albicans and C. norvegensis (21.3 ± 0.63 mm) and C. krusei (20.6 ± 1.5 mm). Moreover, the minimum inhibitory concentrations of both the total extract and the isolated compound were low. The minimum inhibitory concentration of the compound isolated from P. setifera was 0.49 μg/mL against C. kefyr, 0.98 μg/mL against C. albicans and C. norvegensis and 1.95 μg/mL against C. krusei. The oral dosing of the extract and the isolated compound did not show any significant effect on the activity of alanine aminotransferase, aspirate aminotransferase and the levels of blood urea and serum creatinine. Copyright © 2017 John Wiley & Sons, Ltd.
Candida krusei
Candida parapsilosis
Candida dubliniensis
Candida glabrata
Cite
Citations (1)