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    The cellular growth of adipose tissue from Dorset Horn x Merino wethers was characterized by both hypertrophy and hyperplasia of adipose cells until the sheep were c. 11 months old, after which hypertrophy of existing adipose cells was solely responsible for increases in adipose tissue mass. Omental adipose tissue contained larger adipose cells than either the perirenal or subcutaneous sites. The weight of fat which had been deposited in the boneless carcass meat and the internal adipose tissue depots were linearly and positively correlated with the volume of the adipose cells. Decreases in the mass of adipose tissue, which accompanied nutritional restriction, were due to decreased adipose cell size, since no change was observed in the number of adipose cells per carcass after loss in weight. The cellularity characteristics of rehabilitated sheep were similar to those of sheep which had undergone continuous growth.
    3T3-L1
    Adipose tissue macrophages
    Citations (39)
    Adipose tissue can be found throughout the body but is primarily distributed in several subcutaneous and visceral locations or “depots” (1). The general distribution pattern and location of adipose tissue depots and the proportion of total body fat that each depot represents varies widely between species. In humans and swine the subcutaneous depot represents a much larger proportion of total adipose tissue than in rodents. In fact, several distinct layers of subcutaneous adipose tissue are present in humans and swine. Excessive adipose tissue accumulation in humans is often associated with the adverse health consequences of the metabolic syndrome (2). An adipose tissue distribution pattern favoring visceral adipose tissue often is implicated in this syndrome.
    Subcutaneous adipose tissue
    Subcutaneous fat
    GH has profound effects on the amount and distribution of adipose tissue. GHD in both children and adults is accompanied by an increased amount of adipose tissue and by the abdominal predominance of adipose tissue. In contrast, treatment with GH reduces adipose tissue, and redistributes adipose tissue from abdominal to peripheral depots.
    Abdominal fat
    Fat distribution
    Citations (43)
    Syngeneic adipose tissue grafts showed evidence of necrosis or lack of vascularization in intact, adult (BALB/c/Ki x Ce/Ki) F1 hybrid mice between 2 and 9 months after transplantation. One or more fat depots were surgically removed to produce a deficit in the total adipose tissue mass, and this resulted in an increased number of vascularized and viable adipose tissue grafts in similar genetic hosts during a comparable period. The anatomically dispersed fat depots appear to be under some form of self-regulation and integrated into a total adipose tissue mass.
    Citations (29)
    An improved method of extracting the total RNA from ventral subcutaneous adipose tissue,mesentery adipose tissue and tailhead adipose tissue of sheep was developed and the expression and tissue distribution of the fatty acid synthase(FAS)gene in sheep were investigated.The result showed that:total RNA extracted from adipose tissue by the improved method was pure,integral,high quality and without chromosome DNA contamination.The expression of FAS mRNA in the ventral subcutaneous adipose tissue were significantly different compared with tailhead adipose tissue and mesentery adipose tissue(P0.01),but there was no significant difference between tailhead adipose tissue and mesentery adipose tissue(P0.05).
    Subcutaneous adipose tissue
    Adipose tissue macrophages
    Citations (0)
    Functional differences in the different types of adipose tissue and the impact of their dysfunction on metabolism are associated with the regional distribution of adipose depots.Here we show a genome-wide comparison between the transcriptomes of one source of subcutaneous and two sources of visceral adipose tissue in the pig using an RNA-seq approach.We obtained ~32.3 million unique mapped reads which covered ~80.2% of the current annotated transcripts across these three sources of adipose tissue.We identified various genes differentially expressed between subcutaneous and visceral adipose tissue, which are potentially associated with the inflammatory features of visceral adipose tissue.These results are of benefit for understanding the phenotypic, metabolic and functional differences between different types of adipose tissue that are deposited in different body sites.
    RNA-Seq
    Citations (31)