Topical effect of allogenous serum rich in growth factors (SRGF) on diabetic skin wound in rat
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The study of diabetic nephropathy in experimental animals generally relies on the chemical induction of the diabetic state. Streptozotocin is commonly employed to that end; however, streptozotocin has an inherent nephrotoxic potential. We studied the effects of both streptozotocin, and diabetes on the kidneys of rats given streptozotocin 60 mg/kg, which was sufficient to induce severe diabetes. Our studies, which utilized both transmission and scanning electron microscopy, considered the pharmacokinetics of streptozotocin in renal tissue, as well as the effect of insulin treatment. Renal tissue kinetics were altered by occluding the renal hilum of one kidney during, and for 5 min after, the administration of streptozotocin. We found that, in contrast to alloxan, streptozotocin caused no detectable renal injury at the dose employed. Previously described renal epithelial papillomas were identified, and were not influenced by altering the renal tissue kinetics of the drug. We conclude that no ‘protection’ procedure is necessary for the kidney when the streptozotocin model of diabetes is employed. In this regard streptozotocin may have greater utility than alloxan.
Nephrotoxicity
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SummaryThe renal oncogenic activity of streptozotocin in male Holtzman rats was significantly decreased by nicotinamide. Adenomas of the kidney were noted in 77% (21/28) of the animals treated with single iv dose of the streptozotocin, 50 mg/kg, while only 18% (5/28) of animals given nicotinamide ip, 350 mg/kg, 10 min before and 180 min after the same dose of streptozotocin had demonstrable renal tumors. Moreover, the renal adenomas induced by streptozotocin alone occurred sooner and were generally larger when compared with those in the animals treated with the nicotinamide-streptozotocin combination.The 50 mg/kg dose of streptozotocin was diabetogenic in all rats, but the diabetic state was not permanent. Spontaneous recovery from the diabetes was first noted after 8 and 10 months of followup, and after 16 months none of the surviving rats were diabetic.The authors gratefully acknowledge the technical assistance of Marie Gottlieb, Elizabeth Perry, and Eleanor M. Ghigna.
Streptozocin
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Changes of plasma glucose levels have been investigated in rats of 50, 70, 90, 110 and 150 g BVV after the intravenous injection of 0, 40, 60, 80 or 100 mg/kg BW of streptozotocin. The effect of streptozotocin was dependent on the dose used and on the size of the animals. With all animal sizes studied, a transient acute hyperglycemic response to streptozotocin was observed at doses lower than those required to produce persistent hyperglycemia. Smaller animals required higher doses of streptozotocin in order to produce changes comparable to those observed in larger animals. (Endocrinology96: 787, 1975)
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2 Abstract: The present study was designed to investigate the characteristics of diabetes mellitus induced by different doses of Streptozotocin in mice as assessed by blood glucose concentration. Swiss albino mice of either sex were intraperitoneally administered single dose of Streptozotocin 180 mg/kg, or 100 mg/kg and multiple low dose of Streptozotocin 40 mg/kg/day. A single injection of Streptozotocin 180 mg/kg was found to produce Type 1 (insulin-dependent diabetes mellitus) and multiple low dose Streptozotocin 40 mg/kg Type 2 (non-insulin-dependent diabetes mellitus). However, Streptozotocin 100 mg/kg failed to produce diabetes mellitus except a sustained hyperglycemia. Blood glucose concentrations were measured at every week after Streptozotocin injection. Multiple low doses of STZ 40 mg/kg, i.p. to mice for five consecutive days have been found suitable model to study long term complications of diabetes.
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Abstract: Aim: This study aimed to establish the dose of streptozotocin required to induce Type -1 diabetes in Wistar rats. Background: Streptozotocin is currently employed worldwide to induce insulin-dependent diabetes mellitus also known as Type 1 diabetes mellitus in experimental animals. Though many reports on the use of streptozotocin induction of diabetes are reported in the literature, they were not reproducible. Moreover, there was no mention of the mortality associated with the same as well as the conditions followed during the study. Materials and Methods: In the present study, the dose, route, and solvent used for streptozotocin were investigated. Various doses of streptozotocin 55, 50, 40, and 35mg/kg dissolved in either freshly prepared cold 10mM sodium citrate buffer (pH 4.5) or normal saline solution was administered intraperitoneally as well as intravenously. For intraperitoneal administration, the dose-volume was 10mL/kg whereas for intravenous administration the dose-volume was 2mL/Kg. Different routes were employed to ascertain the cause of mortality for which an autopsy was performed. Besides mortality rate was also determined. Results and Discussion: The findings of the study revealed that a 35-40mg/kg dose of streptozotocin showed less percentage mortality and successful induction of diabetes. Conclusion: Mortality (10-20%) was observed at a dose of 35-40mg/kg streptozotocin. Therefore, streptozotocin (35-40mg/kg) was confirmed to be safe and effective for the induction of diabetes in Wistar rats. This paper highlights subtle intricacies observed during induction of diabetes in Wistar rats using streptozotocin Keywords: Male Wistar rats, Streptozotocin, Type-1 diabetes, 5% dextrose, Mortality, Normal saline solution.
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Objective To study the experimental diabetes animal model in rats induced by streptozotocin and explore the difference in different ways. Methods The diabetic model of Wistar rats was duplicated with streptozotocin once introperitoneally or intratailvenously. The blood glucose and plasma insulin levels were detected. Results Blood glucose levels in streptozotocin groups were increased significantly as compared with control group (P 0. 01). Plasma insulin levels in streptozotocin groups were decreased significantly as compared with control group (P0. 01), while there was no significant difference between different ways(P0. 05). Conclusion The diabetic model of Wistar rats could be duplicated with streptozotocin once intrapertoneally or intratailvenously,while there was no significant difference between two ways.
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