Closed Genome Sequences and Antibiograms of 16 Pasteurella multocida Isolates from Bovine Respiratory Disease Complex Cases and Apparently Healthy Controls
Gregory P. HarhayDayna M. HarhayJames L. BonoTimothy P. L. SmithSarah F. CapikKeith D. DeDonderMichael D. ApleyBrian V. LubbersBrad J. WhiteRobert L. Larson
2
Citation
8
Reference
10
Related Paper
Citation Trend
Abstract:
Pasteurella multocida is an animal-associated Gram-negative member of the Pasteurellaceae family. It is an opportunistic pathogen and is one of the principal bacterial species contributing to bovine respiratory disease complex (BRDC) in feedlot cattle.Keywords:
Bovine Respiratory Disease
Pasteurella
Bovine respiratory disease is the leading cause of loss to the North American cattle industry. The disease is characterized by a severe fibrinous pneumonia or a suppurative bronchitis and bronchopneumonia. The bacteria most commonly associated with these cases of pneumonia and deaths are Pasteurella haemolytica (PH) and Pasteurella multocida (PM). Attempts to immunize calves with killed bacterins or extracts of these bacteria have been ineffective. Recently several investigators have used live cultures of PH and PM to immunize calves against respiratory disease. The purpose of these investigations was to develop vaccines that effectively immunize calves against pneumonic pasteurellosis. Two vaccines have been developed that contain viable Pasteurella haemolytica (PRECON-PH) or Pasteurella multocida (BOVICON-PM) (A.H. Robins Corporation, Richmond, Va.).
Pasteurella
Pasteurellosis
Bovine Respiratory Disease
Cite
Citations (0)
Pasteurella multocida and Haemophilus parasuis are the main pathogens of porcine respiratory disease syndrome, and often are the common pathogens of porcine pulmonary disease in collectivized pig farms. It has brought great influence on the pig industry and seriously hampered the healthy development of pigs. Therefore, the rapid detection method for Pasteurella multocida and Haemophilus parasuis is the key to its successful prevention and control. In the process of diagnosis, we need to conduct laboratory tests for confirming of preliminary diagnosis, that can be made based on the history, clinical symptoms, pathological changes of necropsy and epidemiological characteristics of the disease. Traditional diagnostic techniques, such as bacterial isolation and immunological test, are time-consuming and laborious, and are not suitable for rapid clinical diagnosis, nor for large-scale epidemiological investigation. Therefore, it is necessary to establish an accurate and rapid method to identify the two common respiratory pathogens in pigs.
In this paper, Pasteurella multocida and Haemophilus parasuis were used as research objects to establish a dual PCR detection method. The main research contents are as follows: A dual PCR assay was established to detect Pasteurella multocida and Haemophilus parasuis simultaneously. By using Pasteurella multocida and Haemophilus parasuis specific primers, and adjusting primer concentration and annealing temperature, a dual PCR method for detecting Pasteurella multocida and Haemophilus parasuis was established.
The rapid detection method for Pasteurella multocida and Haemophilus parasuis established in this study has high specificity and sensitivity, and can realize rapid and accurate identification of pathogenic bacteria in a relatively short period of time, providing a new technical means for rapid detection in clinical and grassroots laboratories
Pasteurella
Isolation
Cite
Citations (0)
Pasteurella multocida is an animal-associated Gram-negative member of the Pasteurellaceae family. It is an opportunistic pathogen and is one of the principal bacterial species contributing to bovine respiratory disease complex (BRDC) in feedlot cattle.
Bovine Respiratory Disease
Pasteurella
Cite
Citations (2)
С помощью разработанной ПЦР изучена частота выявления серогрупп А и D бактерии Pasteurella multocida из легких телят, больных респираторными заболеваниями. Результаты могут быть использованы при планировании противоэпизоотических мероприятий в хозяйствах по производству молока, а также в научных целях при изучении молекулярной эпизоотологии болезней крупного рогатого скота, вызванных бактериями семейства Pasteurellaceae.
Pasteurella
Pasteurellosis
Cite
Citations (0)
Haemophilus somnus, Pasteurella haemolytica, Pasteurella multocida, and Actinobacillus pleuropneumoniae from cattle and pigs with respiratory disease were used to evaluate the RapID NH system (Innovative Diagnostics, Atlanta, Ga.). Minor modifications of the RapID NH system to include animal source and growth requirements would permit the identification of all isolates tested.
Actinobacillus pleuropneumoniae
Pasteurella
Actinobacillus
Pasteurellosis
Pleuropneumonia
Cite
Citations (7)
Abstract The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37°C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida , the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes , Actinobacillus pleuropneumoniae and Haemophilus parasuis , respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.
Pasteurella
Actinobacillus
Actinobacillus pleuropneumoniae
Cite
Citations (1)
Bovine Respiratory Disease
Pasteurella
Broth microdilution
Cite
Citations (19)
Mannheimia haemolytica, formerly known as Pasteurella haemolytica, is a bacterium belonging to the family Pasteurellaceae. It is commonly found in the respiratory tracts of cattle and other ruminant animals, and is a significant pathogen in the cattle industry. This bacterium is known to cause bovine respiratory disease (BRD), which can lead to severe economic losses in the livestock industry.
Bovine Respiratory Disease
Pasteurella
Pasteurellosis
Cite
Citations (0)
Deoxyribonucleic acid (DNA)-DNA hybridization was used to determine the genetic relationships among a variety of previously established or proposed species of Pasteurella and their positions within the family Pasteurellaceae Pohl 1981. Our results indicated that the genus Pasteurella sensu stricto, which can be separated from the Actinobacillus group, consists of at least the following 11 species: Pasteurella multocida, with three subspecies (P. multocida subsp. multocida, P. multocida subsp. septica, and P. multocida subsp. gallicida); Pasteurella dagmatis sp. nov., containing organisms previously labeled Pasteurella "gas," Pasteurella new species 1, or Pasteurella pneumotropica type Henriksen; Pasteurella gallinarum; Pasteurella canis sp. nov., previously labeled P. multocida biotype 6 or "dog type" strains; Pasteurella stomatis sp. nov., which contains Pasteurella strains isolated from dogs and cats; Pasteurella avium (Hinz and Kunjara) Mutters et al. 1985; Pasteurella volantium Mutters et al. 1985, a new species consisting of V-factor-requiring strains that occur in humans and birds; Pasteurella anatis sp. nov.; Pasteurella langaa sp. nov., containing strains previously designated taxa 1 and 4 of Bisgaard; and two new species, which were provisionally designated Pasteurella species A and Pasteurella species B. The previously recognized taxa Pasteurella ureae, Pasteurella haemolytica biotypes A and T, Pasteurella testudinis, and P. pneumotropica biotypes Jawetz and Heyl do not belong to the genus Pasteurella but are more closely related to the Actinobacillus group. The exact taxonomic positions of Pasteurella aerogenes, P. multocida biotype 1, group HB-5, "Pasteurella piscicida," the SP group, and the bovine lymphangitis group are still unknown, but these organisms do not belong to the genus Pasteurella.
Pasteurella
Pasteurellosis
Actinobacillus
Cite
Citations (348)
С помощью разработанной ПЦР изучена частота выявления серогрупп А и D бактерии Pasteurella multocida из легких телят, больных респираторными заболеваниями. Результаты могут быть использованы при планировании противоэпизоотических мероприятий в хозяйствах по производству молока, а также в научных целях при изучении молекулярной эпизоотологии болезней крупного рогатого скота, вызванных бактериями семейства Pasteurellaceae.
Pasteurella
Pasteurellosis
Cite
Citations (0)