Evaluation of Mineral Concentrations in Maternal Serum Before and After Birth and in Newborn Cord Blood Postpartum—Preliminary Study
Rafał KocyłowskiIwona LewickaMariusz GrzesiakZuzanna GajPrzemysław OszukowskiConstantin von KaisenbergJoanna Suliburska
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Cord blood
Umbilical artery
Umbilical cord blood is an important graft source in the treatment of many genetic, hematologic, and immunologic disorders by hematopoietic stem cell transplantation. Millions of cord blood units have been collected and stored for clinical use since the inception of cord blood banking in 1989. However, the use of cord blood in biomedical research has been limited by access to viable samples. Here, we present a cost-effective, self-sustaining model for the procurement of fresh umbilical cord blood components for research purposes within hospital-affiliated academic institutions.
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Umbilical artery
Base excess
Arterial blood
Arterial pH
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Background: As the new born is delivered and the umbilical cord divided, blood can be collected from the segment of cord, still attached to the placenta are known as “umbilical cord blood” stem cell. The blood collected in the umbilical cord is said to be a rich origin of stem. The blood contains stem cells which are also known as hematopoietic cells and these cells can convert into any types of organs in the body. These stem cells collected from the umbilical cord can heal genetic diseases related to blood and immune system like cancer, blood disorders and several life –threatening diseases. Objectives: Assess the level of knowledge regarding stem cells and cord blood banking among antenatal mothers. Find out the association between the level of knowledge regarding stem cells and cord blood banking among antenatal mothers with their selected demographic variables.
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Placenta cord banking
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To find out the influence of nuchal cord on neonatal acid-base balance we obtained 117 umbilical artery blood samples from full-term newborns immediately after cord clamping. In 29 cases umbilical cord entanglement was observed while delivery. For each case we matched control case with basic obstetrical factors comparable. In those two groups no significant difference between mean values of acid-base balance parameters was found.
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Acid–base homeostasis
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Single umbilical artery
Aplasia
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Objective To study the age-specific morphology of fetal umbilical vein and to provide evidence for clinical wide use. Methods Fresh umbilical veins obtained from 33 cases of 32-42 weeks old were sliced with paraffin section methods. The section preparations stained with HE and special methods was measured using light microscope and image analysis system. Results Umbilical vein wall, media membrane, and smooth muscle widened with age. The cellular nuclear numerical density(CNND)and the cellular nuclear area density(CNAD)are the greatest at the age of 37-40 weeks.Conclusion The smooth muscle, CNND and CNAD are increased with fetal age, suggesting that it is advisable for the choice of 37～40-week-old umbilical vein as grafts.
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The plasma levels of 20 free amino acids in the umbilical veins and umbilical arteries of 8 premature (29–36 weeks gestation) and 16 mature (38–42 weeks gestation) newborn infants were measured at delivery. In premature newborn infants, most of the 20 amino acids were significantly higher in the umbilical vein than in the umbilical artery. Only glutamic acid was significantly lower in the umbilical vein than in the umbilical artery. In mature newborn infants, 7 (Ala, Lys, Leu, Val, He, Phe and His) of the 20 plasma amino acids were significantly higher and 4 (Glu, Gly, Ser and Om) were significantly lower in the umbilical vein than in the umbilical artery. These results indicate that the relative contribution of individual amino acids to the placental supply of nitrogen to the human fetus discernibly changes with increasing fetal age during the last trimester of gestation.
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Effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelial cells
Objective: To observe the effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelia cells (HUVEC). Methods: Primary and passaged HUVEC were cultured in the medium with 20% mixed umbilical cord blood plasma and their purities were evaluated by Ⅷ:Ag and UEA Ⅰ.Proliferative activities of HUVEC were compared in the medium containing different amounts of mixed umbilical cord blood plasma by methyl thiazolyl tetrazolium(MTT) assay. Results: 20% mixed umbilical cord blood plasma can maintain cell viability in the medium without endothelial cell growth factor(ECGF). The purity of endothelial cells was 93.1%. Conclusion: 20 portion mixed umbilical cord blood plasma can be a substitute for ECGF and used successfully in the culture of HUVEC to obtain high purity and passagable endothelial cells.
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We have evaluated the effects of propofol and its relationship with K+ channels on human isolated umbilical vessels.Umbilical vessel rings were suspended in isolated organ baths containing Krebs-Ringer solution. In the first series of experiments the effect of propofol (10(-9)-10(-4) M) was examined in a concentration-dependent manner on umbilical vessels precontracted with KCl (60 mmol). In the second series, these effects were studied in the presence of tetraethylammonium.A mild contraction was produced by low dose propofol in both precontracted umbilical artery and umbilical vein segments. 10(-4) M propofol caused significant relaxation in both umbilical artery and umbilical vein. The relaxation response was significantly reduced by the addition of 10(-1) M tetraethylammonium.These results suggested that the responses of propofol on KCl-induced contractions of both umbilical artery and vein were dose dependent, and this effect involved Ca2+ activated K+ channels.
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