DAYA TOLAK INFUSA DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb.) TERHADAP PELETAKAN TELUR NYAMUK Aedes spp
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Aedes spp larval resistance against chemical insecticide has been reported in many country. The new methods that can be used to control the mosquito populations as a dengue fever vector and alternative compound which is safe for environtment and can’t cause the resistance need to be found. Pandan leaves have aromatic fragrant that can be expected as a oviposition deterrent effect against Aeses spp mosquito. To know oviposition deterrent effects and optimum consentration of pandan leaves (Pandanus amaryllifolius Roxb.) infusa against Aedes spp. This research was an experimental research using six groups of concentration. There was 0 mL/L (control), 3 mL/L, 3,5 mL/L, 4 mL/L, 4,5 mL/L and 5 mL/L. Every groups would be repeated by fourth. Ovitraps with different concentration of pandan leaves infusa placed at research location. The mosquito eggs that caught at the ovitrap would be counting and identifying in the laboratorium. Data was analyzed using One Way Anova test via computer program SPSS 19.0 and then continued by LSD test with 95% confidence level. The repellency percentage at 3 mL/L concentration is 30.35%; 3,5 mL/L is 55.35%; 4 mL/L is 65.17%; 4,5 mL/L is 82.14% and 5 mL/L is 100% with significancy value is 0,000 (p<0,05).Cite
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Abstract Aedes aegypti (L.) (Diptera: Culicidae) is a diurnal feeder that lives in close association with human populations. It is the principal vector of yellow fever, dengue fever and the Zika Virus. Issues of arboviral diseases have been on the ascendency in most countries including Ghana where Aedes mosquito is the main vector of yellow fever. A comparative study of the biting behavior of Ae. aegypti and the identification of subspecies were undertaken using molecular technique. Standard human landing technique was used to collect both indoor and outdoor biting mosquitoes at three zones located in the Upper East (Bolgatanga), Upper West (Nadowli), and Northern (Damongo) Regions of Ghana during the dry and rainy seasons between 0600 and 1800 Greenwich Mean Time (GMT). All collected mosquitoes were identified morphologically using taxonomic keys. random amplified polymorphic DNA polymerase chain reaction was used to categorize Ae. aegypti into subspecies. Adult female Aedes mosquitoes identified formed 62% (n = 1,206) of all female mosquitoes collected. Aedes aegypti 98% and Aedes vittatus 2% were the only Aedes species identified. Bolgatanga recorded the largest number of Ae. aegypti 42%, whereas Nadowli 22% recorded the least. Aedes vittatus was observed in Nadowli. Aedes aegypti exhibited a bimodal biting behavior peaking at 0600–0800 GMT and 1500–1600 h GMT. Molecular findings revealed 69% Ae. aegypti aegypti and 31% Ae. aegypti formosus as the two subspecies (n = 110). This information is important for implementing effective vector control programs in the three regions of the northern Ghana.
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Aedes aegypti mosquitoes are the primary transmitters of dengue fever, urban yellow fever and chikungunya viruses. This mosquito has developed resistance to the insecticides currently used to control their populations. These chemical insecticides are harmful to the environment and can have negative effects on human health. Rhamnolipids are environmentally compatible biological surfactants, but their insecticidal activity has not been extensively studied. The present study evaluated the potential larvicidal, insecticidal and repellent activities of rhamnolipids against Aedes aegypti. At concentrations of 800, 900 and 1000 mg/L, rhamnolipids eliminated all mosquito larvae in 18 hours and killed 100% of adults at 1000 mg/L. According to the results it may be conclude that rhamnolipids should be applied to control larvae and mosquitos besides present the repellency activity against Aedes aegypti.
Mosquito control
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Dengue fever is one of the dangerous diseases that can lead to a death in a short time and often lead to outbreaks. It is one of the major public health problems in Indonesia. Dengue is transmitted to humans by the bite of the female Aedes mosquito infected with dengue virus. The main transmission vector was Aedes aegypti mosquito that is able to proliferate well in many places water shelter. This study aims to determine the relationship and create an environmental mapping with the presence of the Aedes aegypti larvae in endemic regions of Makassar. The type of research is analytic survey with a cross-sectional design that conducted by observing the container as a breeding Aedes aegypti site in 200 homes of 5 endemic sub regencies. Sampling technique is done by proportional random sampling. The results showed that there was a relationship between the presence of humidity by the larvae of Aedes aegypti (p value: 0.027). Whereas no relationship between the temperature and pH to the existence of Aedes aegypti larvae (p value; 0, 345 and .514). To sum up, based on the mapping, it was found that the highest level of larvae was in Tamalanrea sub district. It is suggested to the community to reduce the potential breeding sites mainly water containers that used daily
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Abstract The cosmopolitan mosquito Aedes aegypti is a vector of harmful arboviruses. Pyrethroid insecticides are used to reduce adult populations and prevent the spread of disease. Pyrethroids target the insect voltage-gated sodium channel (VGSC). Collectively, mutations in Vgsc that confer resistance are referred to as knock-down resistance or kdr. There are numerous kdr mutations found in A. aegypti Vgsc, and there is co-occurrence of some mutations. Full-length cDNA sequences have identified nine known kdr (e.g., 1534C) alleles. The 1534C allele is among the most common kdr alleles, but allele frequencies between populations vary considerably. We used the 1534C:RK strain, which has the 1534C (kdr) allele in the genetic background of the insecticide susceptible Rockefeller (ROCK) strain, and conducted population cage experiments to assess the potential intrinsic fitness cost of the 1534C allele relative to the susceptible allele (F1534) in the ROCK strain. Individuals were genotyped across generations using allele specific PCR. A fitness cost of the 1534C allele was detected across seven generations of mosquitos reared in the absence of insecticide selection pressure. The decrease in allele frequency was not due to drift. Comparison of our results to previous studies suggests that the magnitude of the fitness cost of kdr alleles in the absence of insecticide is disconnected from the level of resistance they confer, and that the fitness costs of different kdr alleles can be variable.
Insecticide Resistance
Pesticide resistance
Mosquito control
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Abstract Chikungunya and Zika are arboviruses transmitted by the mosquito Aedes aegypti . Mosquito fecundity and egg viability are important parameters of vectorial capacity. Here we aim to understand, comparatively, the effects of Chikungunya virus (CHIKV) and Zika virus (ZIKV) infections on the fecundity and fertility of young and old Aedes aegypti females. Using artificial infection blood feeding experiments we observed that both CHIKV and ZIKV do not alter the number of eggs laid when compared to uninfected females, although the egg fertility significantly decreases in both young and old CHIKV-infected females. There is an upward trend of null females (infertile females) from 2.1% in young to 6.8% in old ZIKV-infected females. Together, our data revealed that CHIKV and ZIKV affects differently Ae. aegypti physiology, that may be related to different viral spread in nature.
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Dengue Hemmoragic Fever (DHF) is one of the public health problems in Indonesia. The disease is caused by dengue virus that is transmitted to humans through the bite of Aedes aegypti mosquito. Aedes aegypti mosquito eggs usually live in clean water puddles. The factors that affect the viability of Aedes aegypti mosquito eggs are water pH and egg structure. Alum can decrease the pH of water and chlorine can damage the egg protein structure. Both chemicals are used as water purifiers. The purpose of this research is to analyze the difference between 100 ppm alum water concentration and 10 ppm chlorine concentration to Aedes aegypti mosquito egg hatchability. The research was carried out by experimental method. The research was done with 3 treatments, those were 100 ppm alum water concentration, chlorine 10 ppm concentration, and 1 control. At each concentration were given 25 Aedes aegypti mosquito eggs. The reseach was conducted for 3 days and done throught 3 studies in parallel. The result showed that the chlorine water concentration 10 ppm concentration was more effective in inhibiting the hatching of Aedes aegypti mosquito eggs compared with 100 ppm alum water concentration. The data of the research were analyzed using Kruskal-Wallis test which showed the value of p <0,05 which stated that there is difference of the hatchability of Aedes aegypti mosquito egg. Based on this research it can be concluded that 10 ppm chlorine water concentration was more effective in inhibiting the hatchability of Aedes aegypi mosquito eggs, so chlorine can be used to decrease the density of Aedes aegypti mosquito.
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The Aedes aegypti mosquito is the main vector of dengue fever in Indonesia. Control using synthetic chemical insecticides has a bad impact. Vegetable insecticides from salam leaf extract are a solution to reduce the negative impact of synthetic chemical insecticides. the aim of this study is To determine the efficacy of salam leaf extracts against the death of the Aedes aegypti mosquito. This research was carried out experimentally with the Post-test design with a control group (Post Test Only Control Group), namely, the experimental group receive treatment or intervention (X) followed by a second measurement or observation (O2), with the study population being all Aedes aegypti mosquitoes which were bred in the Laboratory and Workshop of the Environmental Health Department of the Health Polytechnic of the Ministry of Health of Aceh. The results of the efficacy test of salam leaf ethanol extract by spraying obtained LC 50 = 69.103% and LC 90 = 299.972%, by fogging LC 50 = 43.937% and LC 90 = 311.879%. Salam leaves are very effective, the efficacy of Aedes aegypti mosquito mortality is carried out by spray or fogging at a concentration of 90 LC. It is hoped that the results of this study can be applied in the community for efforts to eradicate Aedes aegypti mosquitoes by using vegetable insecticides that can reduce the effects of synthetic chemicals.
Mosquito control
Christian ministry
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Larvicide
Mosquito control
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