Associations of Osteocalcin, Osteoprotegerin, and Calcitonin with Inflammation Biomarkers in Atherosclerotic Plaques of Coronary Arteries
Yа. V. PolonskayaЕ. В. КаштановаИ. С. МурашовА. М. ВолковА. В. КургузовA. M. ChernyavskyYu. I. Ragino
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Osteopontin
Proinflammatory cytokine
Coronary arteries
Objective: Ectomesenchymal stem cells (EMSCs) are considered to be able to differentiate toward a cementoblast/osteoblast phenotype. This study is to investigate Osteopontin and Osteocalcin mRNA expression of Rat ectomesenchymal stem cells under inducing condition of EMPs EMD in vitro. Methods: Cultured EMSCs were exposed to the EMPs- or EMD- or (EMPs+EMD)- conditioned culture media. Osteoblast MC3T3-E1 served as positive control (P) and non-induced EMPs as negative control (N). Then Osteopontin and Osteocalcin mRNA expression were measured by RT-PCR technique. Result: 1) There was expression of Osteopontin mRNA in group EMPs, group EMD and group (EMPs+EMD) after induction for 3 weeks ; 2) There was expression of Osteocalcin mRNA only observed in group (EMPs+EMD). Conclusion: The cultured Rat EMSCs kept non-osteogenic differentiated state by itself; but may have osteogenic or cementogenic potential testified by expression of Osteopontin and Osteocalcin mRNA when induced by EMPs.
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Background and Purpose— The aim of this study was to compare the concentration of osteoprotegerin (OPG), receptor activator of nuclear factor κB ligand (RANKL), and osteopontin (OPN) in stable (asymptomatic) and unstable (symptomatic) carotid atherosclerosis. In addition, we were interested in the effect of angiotensin II blockade on the secretion of these proteins by unstable atherosclerosis. Methods— Endarterectomy samples removed from patients with recent (within 6 weeks) or no previous focal neurological symptoms were assessed by immunohistochemistry, Western analysis, and explant culture. Concentrations of OPG, RANKL, and OPN were measured by mean optical density (MOD), densitometry of protein bands, and enzyme-linked immunosorbent assay of supernatants from explant culture, and compared between symptomatic and asymptomatic patients. Results— The concentration of OPG and OPN within the proximal internal carotid (PIC) part of the endarterectomy specimen removed from symptomatic patients was elevated 2...
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Endarterectomy
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Abstract Objectives: Study of circulating and synovial levels of osteocalcin, osteoprotegerin and tumor necrosis factor-alpha (TNF-α) in patients with different stages of knee osteoarthritis and correlation analysis of these parameters with disease severity. Methods: We enrolled 20 patients with different stages of knee osteoarthritis. The IKDC score (International Knee Documentation Comittee, 2000) was determined for each patient. Based on these data patients were divided into two groups: group I (advanced osteoarthritis) and group II (early osteoarthritis). Serum and synovial fluid levels of osteocalcin, osteoprotegerin, TNF-α were determined. Results: For the entire group the level of osteocalcin in the serum showed higher values than in the synovial fluid. We found statistically significant differences in the serum levels of osteocalcin between the two groups (group I: 2.18 ± 0.54 ng/ml, group II: 6.07 ± 1.98 ng/ml, p = 0.019). Serum and synovial osteocalcin in the whole study lot could not be correlated with the disease score, however we observed a tendency towards significant negative correlation between the serum osteocalcin and IKDC score for group I and between synovial osteocalcin and IKDC score in group II. In the entire group, synovial osteoprotegerin concentration was six times higher than the serum osteoprotegerin level (p <0.0001) and TNF-α showed higher circulating levels than local concentrations. Conclusions: In the advanced osteoarthritis group the serum and synovial osteocalcin show lower values than in the early osteoarthritis group, which means that as the disease progresses, bone anabolism decreases. In the case of osteoprotegerin, no significant difference between the two groups was detected.
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Σκοπός της μελέτης: Μολονότι σε μελέτες συσχετίζονται τα βιομόρια οστεοποντίνη (osteopontin - OPN) και οστεοπροτεγερίνη (osteoprotegerin - OPG) με την ύπαρξη ΑΚΑ στον άνθρωπο, εντούτοις απουσιάζουν αναφορές που να διερευνούν τις μεταβολές στις συγκεντρώσεις τους σε σχέση με την θεραπευτική του αποκατάσταση. Στην παρούσα μελέτη, προσδιορίσθηκαν οι συγκεντρώσεις αυτών των μορίων (OPN, OPG) στον ορό ασθενών με ΑΚΑ, τόσο προεγχειρητικά όσο και μετεγχειρητικά (είτε με κλασσική χειρουργική επέμβαση είτε ενδαγγειακά). Επιπροσθέτως διερευνήθηκε η πιθανή συσχέτισή τους με την ανατομική έκταση του ΑΚΑ. Υλικό και Μέθοδος: Η προοπτική αυτή μελέτη περιλαμβάνει εβδομήντα τέσσερεις (n=74) ασθενείς με Ανεύρυσμα Κοιλιακής Αορτής (ΑΚΑ) υπονεφρικής μοίρας που κρίθηκαν κατάλληλοι και υπεβλήθησαν σε προγραμματισμένη επέμβαση αποκατάστασης (χειρουργική ή ενδαγγειακή). Όλα τα ανευρύσματα ταξινομήθηκαν, βάσει της ανατομικής τους έκτασης, σε πέντε τύπους (Α-Ε) βάσει EUROSTAR. Σε όλους τους ασθενείς, ελήφθησαν δείγματα φλεβικού αίματος, τόσο προεγχειρητικά όσο και μετεγχειρητικά (μια εβδομάδα μετά), ώστε να προσδιορισθούν, στον ορό, οι αντίστοιχες συγκεντρώσεις των μορίων OPN και OPG. Για τη συγκριτική μελέτη χρησιμοποιήθηκαν οι αντίστοιχες συγκεντρώσεις στον ορό των ίδιων μορίων μιας ομάδας ελέγχου (control group), είκοσι (n=20) ασθενών που είχαν προγραμματισθεί να υποβληθούν σε επέμβαση αποκατάστασης βουβωνοκήλης. Αποτελέσματα: Τα προεγχειρητικά επίπεδα της OPN των ασθενών ήταν υψηλότερα συγκριτικά με τα αντίστοιχα εκείνων της ομάδας ελέγχου, ανεξαρτήτως της ανατομικής έκτασης του ανευρύσματος, ενώ δεν παρατηρήθηκε στατιστικώς σημαντική μεταβολή των προεγχειρητικών επιπέδων της OPG. Μετεγχειρητικά, σε όλους τους ασθενείς με ΑΚΑ, παρατηρήθηκε στατιστικά σημαντική αύξηση των μετεγχειρητικών συγκεντρώσεων, τόσο της OPN (p=0,001) όσο και της OPG (p=0,01) συγκριτικά με τις αντίστοιχες προεγχειρητικές συγκεντρώσεις. Ειδικότερα όμως, ενώ τα μετεγχειρητικά επίπεδα της OPN ήταν αυξημένα είτε εφαρμόσθηκε η ανοικτή χειρουργική (p=0,025) είτε η ενδαγγειακή αποκατάσταση (p=0,017), εντούτοις τα μετεγχειρητικά επίπεδα της OPG σημείωσαν στατιστικώς σημαντική αύξηση μόνο σε όσους χειρουργήθηκαν «ανοικτά» (p=0,02). Από τη συγκριτική μελέτη των μετεγχειρητικών.
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Purpose: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-El osteoblastic cell line, particularly on the expression of osteocalcin and osteopontin. Materials and Methods: Cells were irradiated with a single dose of 0.5, 1,4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. After the specimens were harvested, RNA was extracted on the 3rd, 7th, 14th, and 21st day after irradiation. The RNA strands were reverse-transcribed and the resulting cDNAs were subjected to amplification by PCR. Results: The irradiated cells demonstrated a dose-dependent increase in osteocalcin and a dose-dependent decrease in osteopontin mRNA expression compared with the non-irradiated control group, The amount of osteocalcin mRNA expression decreased significantly at the 3rd day after irradiation of 0,5, 1,4, and 8 Gy, and also decreased significantly at the 3rd, 14th, and 21 st day after irradiation in the 8 Gy exposed group compared with the control group, The degree of osteopontin mRNA expression increased significantly at the 7th day after irradiation of 0,5, 1,4, and 8Gy, Conclusion: These results showed that each single dose of 0,5, 1, 4, and 8 Gy influenced the mRNA expression of osteocalcin and osteopontin associated with the calcification stage of osteoblastic cells, suggesting that each single dose affected bone formation at the cell level.
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Bone morphogenetic proteins (BMPs) are a group of structurally related proteins in the transforming growth factor‐beta (TGF‐beta) family which have been shown to stimulate bone formation in vivo. The hypothesis of this study was that osteoblast‐like cells (OBs) treated with BMP would have similar levels of secretion of osteopontin (OPN) and osteocalcin (OCN) to that of OBs treated with IGF‐1. Specifically, the aims of this study were to evaluate osteoblast‐like cells (MG‐63 cell line) for secretion of both OPN and OCN after treatment with DBM, OP‐1, or insulin‐like growth factor‐1 (IGF‐1) compared to control, at 24, 48, and 72 hours. After each incubation period, ELISA kits were used to determine the levels of osteopontin and osteocalcin production. The results clearly demonstrated a rise of OCN at 48 hours and a fall at 72 hours for all samples, including control groups. However, there was no significant difference between groups (p > 0.05). With the OPN assay, results showed no significant difference between groups until 72 hours (p = 0.022), where the IGF‐1 group was significantly higher than the Control and (p = 0.021, respectively). This information is important for understanding the signaling pathways that may be innervated in the osteoblast following stimulation with growth factors.
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