Homocysteine attenuates the expression of osteocalcin but enhances osteopontin in MC3T3-E1 preosteoblastic cells
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Objective: Ectomesenchymal stem cells (EMSCs) are considered to be able to differentiate toward a cementoblast/osteoblast phenotype. This study is to investigate Osteopontin and Osteocalcin mRNA expression of Rat ectomesenchymal stem cells under inducing condition of EMPs EMD in vitro. Methods: Cultured EMSCs were exposed to the EMPs- or EMD- or (EMPs+EMD)- conditioned culture media. Osteoblast MC3T3-E1 served as positive control (P) and non-induced EMPs as negative control (N). Then Osteopontin and Osteocalcin mRNA expression were measured by RT-PCR technique. Result: 1) There was expression of Osteopontin mRNA in group EMPs, group EMD and group (EMPs+EMD) after induction for 3 weeks ; 2) There was expression of Osteocalcin mRNA only observed in group (EMPs+EMD). Conclusion: The cultured Rat EMSCs kept non-osteogenic differentiated state by itself; but may have osteogenic or cementogenic potential testified by expression of Osteopontin and Osteocalcin mRNA when induced by EMPs.
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To study the role of osteopontin, we did cell adhesion and ALP assays of rat bone marrow osteoblastic cells (RBMO) on collagen Type I and osteopontin surfaces. The RBMO proved to adhere much more strongly to the osteopontin and to have higher ALP activity on the osteopontin, which suggests that pre-osteoblasts differentiate into osteoblasts that form bone by recognizing osteopontins.
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Bone sialoprotein
Osteopontin
Calvaria
Type I collagen
Osteoid
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Osteopontin
Phosphoprotein
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Osteopontin
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Purpose: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-El osteoblastic cell line, particularly on the expression of osteocalcin and osteopontin. Materials and Methods: Cells were irradiated with a single dose of 0.5, 1,4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. After the specimens were harvested, RNA was extracted on the 3rd, 7th, 14th, and 21st day after irradiation. The RNA strands were reverse-transcribed and the resulting cDNAs were subjected to amplification by PCR. Results: The irradiated cells demonstrated a dose-dependent increase in osteocalcin and a dose-dependent decrease in osteopontin mRNA expression compared with the non-irradiated control group, The amount of osteocalcin mRNA expression decreased significantly at the 3rd day after irradiation of 0,5, 1,4, and 8 Gy, and also decreased significantly at the 3rd, 14th, and 21 st day after irradiation in the 8 Gy exposed group compared with the control group, The degree of osteopontin mRNA expression increased significantly at the 7th day after irradiation of 0,5, 1,4, and 8Gy, Conclusion: These results showed that each single dose of 0,5, 1, 4, and 8 Gy influenced the mRNA expression of osteocalcin and osteopontin associated with the calcification stage of osteoblastic cells, suggesting that each single dose affected bone formation at the cell level.
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Objective To explore the effect of osteopontin(OPN) as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence.Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer(observation group) and 22 cases of normal subjects(control group);semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues.The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed.Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group(P0.01).The OPN mRNA expression and the serum level in patients with recurrence one year after operation were higher than those without recurrence(P0.01).Conclusion Osteopontin is one of the tumor markers and it could be used in diagnosis and prognosis evaluation of colon cancer.
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Bone morphogenetic proteins (BMPs) are a group of structurally related proteins in the transforming growth factor‐beta (TGF‐beta) family which have been shown to stimulate bone formation in vivo. The hypothesis of this study was that osteoblast‐like cells (OBs) treated with BMP would have similar levels of secretion of osteopontin (OPN) and osteocalcin (OCN) to that of OBs treated with IGF‐1. Specifically, the aims of this study were to evaluate osteoblast‐like cells (MG‐63 cell line) for secretion of both OPN and OCN after treatment with DBM, OP‐1, or insulin‐like growth factor‐1 (IGF‐1) compared to control, at 24, 48, and 72 hours. After each incubation period, ELISA kits were used to determine the levels of osteopontin and osteocalcin production. The results clearly demonstrated a rise of OCN at 48 hours and a fall at 72 hours for all samples, including control groups. However, there was no significant difference between groups (p > 0.05). With the OPN assay, results showed no significant difference between groups until 72 hours (p = 0.022), where the IGF‐1 group was significantly higher than the Control and (p = 0.021, respectively). This information is important for understanding the signaling pathways that may be innervated in the osteoblast following stimulation with growth factors.
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Bone remodeling
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Osteopontin
Bone matrix
Matrix (chemical analysis)
matrix Gla protein
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